{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T15:10:38Z","timestamp":1772809838859,"version":"3.50.1"},"reference-count":51,"publisher":"Rockefeller University Press","issue":"6","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2003,3,17]]},"abstract":"<jats:p>We report the development and characterization of an in vitro system for the formation of filopodia-like bundles. Beads coated with actin-related protein 2\/3 (Arp2\/3)\u2013activating proteins can induce two distinct types of actin organization in cytoplasmic extracts: (1) comet tails or clouds displaying a dendritic array of actin filaments and (2) stars with filament bundles radiating from the bead. Actin filaments in these bundles, like those in filopodia, are long, unbranched, aligned, uniformly polar, and grow at the barbed end. Like filopodia, star bundles are enriched in fascin and lack Arp2\/3 complex and capping protein. Transition from dendritic to bundled organization was induced by depletion of capping protein, and add-back of this protein restored the dendritic mode. Depletion experiments demonstrated that star formation is dependent on Arp2\/3 complex. This poses the paradox of how Arp2\/3 complex can be involved in the formation of both branched (lamellipodia-like) and unbranched (filopodia-like) actin structures. Using purified proteins, we showed that a small number of components are sufficient for the assembly of filopodia-like bundles: Wiskott-Aldrich syndrome protein (WASP)\u2013coated beads, actin, Arp2\/3 complex, and fascin. We propose a model for filopodial formation in which actin filaments of a preexisting dendritic network are elongated by inhibition of capping and subsequently cross-linked into bundles by fascin.<\/jats:p>","DOI":"10.1083\/jcb.200208059","type":"journal-article","created":{"date-parts":[[2003,3,17]],"date-time":"2003-03-17T14:06:14Z","timestamp":1047909974000},"page":"951-962","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":219,"title":["Formation of filopodia-like bundles in vitro from a dendritic network"],"prefix":"10.1083","volume":"160","author":[{"given":"Danijela","family":"Vignjevic","sequence":"first","affiliation":[{"name":"1Department of Cell and Molecular Biology, Northwestern University Medical School, Chicago, IL 60611"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Defne","family":"Yarar","sequence":"additional","affiliation":[{"name":"2Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew D.","family":"Welch","sequence":"additional","affiliation":[{"name":"2Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Peloquin","sequence":"additional","affiliation":[{"name":"1Department of Cell and Molecular Biology, Northwestern University Medical School, Chicago, IL 60611"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatyana","family":"Svitkina","sequence":"additional","affiliation":[{"name":"1Department of Cell and Molecular Biology, Northwestern University Medical School, Chicago, IL 60611"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gary G.","family":"Borisy","sequence":"additional","affiliation":[{"name":"1Department of Cell and Molecular Biology, Northwestern University Medical School, Chicago, IL 60611"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[2003,3,17]]},"reference":[{"key":"2023072213584181700_BIB1","doi-asserted-by":"crossref","first-page":"4177","DOI":"10.1091\/mbc.10.12.4177","volume":"10","year":"1999","journal-title":"Mol. Biol. Cell."},{"key":"2023072213584181700_BIB2","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1016\/S0092-8674(02)00731-6","volume":"109","year":"2002","journal-title":"Cell."},{"key":"2023072213584181700_BIB3","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/S0955-0674(99)00063-0","volume":"12","year":"2000","journal-title":"Curr. Opin. Cell Biol."},{"key":"2023072213584181700_BIB4","doi-asserted-by":"crossref","first-page":"4908","DOI":"10.1073\/pnas.96.9.4908","volume":"96","year":"1999","journal-title":"Proc. Natl. Acad. Sci. USA."},{"key":"2023072213584181700_BIB5","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1038\/35040061","volume":"1","year":"2000","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"2023072213584181700_BIB6","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/S0960-9822(01)00022-7","volume":"11","year":"2001","journal-title":"Curr. Biol."},{"key":"2023072213584181700_BIB7","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1002\/1097-0169(200102)48:2<109::AID-CM1002>3.0.CO;2-G","volume":"48","year":"2001","journal-title":"Cell Motil. Cytoskeleton."},{"key":"2023072213584181700_BIB8","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/S0955-0674(99)00062-9","volume":"12","year":"2000","journal-title":"Curr. Opin. Cell Biol."},{"key":"2023072213584181700_BIB9","doi-asserted-by":"crossref","first-page":"1659","DOI":"10.1091\/mbc.6.12.1659","volume":"6","year":"1995","journal-title":"Mol. Biol. Cell."},{"key":"2023072213584181700_BIB10","doi-asserted-by":"crossref","first-page":"1319","DOI":"10.1083\/jcb.146.6.1319","volume":"146","year":"1999","journal-title":"J. Cell Biol."},{"key":"2023072213584181700_BIB11","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1038\/ncb718","volume":"4","year":"2002","journal-title":"Nat. Cell Biol."},{"key":"2023072213584181700_BIB12","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1128\/MMBR.65.4.595-626.2001","volume":"65","year":"2001","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"2023072213584181700_BIB13","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1146\/annurev.biochem.70.1.649","volume":"70","year":"2001","journal-title":"Annu. Rev. Biochem."},{"key":"2023072213584181700_BIB14","doi-asserted-by":"crossref","first-page":"979","DOI":"10.1016\/S0960-9822(99)80428-X","volume":"9","year":"1999","journal-title":"Curr. Biol."},{"key":"2023072213584181700_BIB15","doi-asserted-by":"crossref","first-page":"11437","DOI":"10.1074\/jbc.270.19.11437","volume":"270","year":"1995","journal-title":"J. Biol. Chem."},{"key":"2023072213584181700_BIB16","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1101\/SQB.1982.046.01.057","volume":"46","year":"1982","journal-title":"Cold Spring Harb. Symp. Quant. Biol."},{"key":"2023072213584181700_BIB17","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1002\/bies.10070","volume":"24","year":"2002","journal-title":"Bioessays."},{"key":"2023072213584181700_BIB18","doi-asserted-by":"crossref","first-page":"1324","DOI":"10.1083\/jcb.99.4.1324","volume":"99","year":"1984","journal-title":"J. Cell Biol."},{"key":"2023072213584181700_BIB19","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/S0896-6273(00)81092-2","volume":"22","year":"1999","journal-title":"Neuron."},{"key":"2023072213584181700_BIB20","doi-asserted-by":"crossref","first-page":"1245","DOI":"10.1083\/jcb.144.6.1245","volume":"144","year":"1999","journal-title":"J. Cell Biol."},{"key":"2023072213584181700_BIB21","doi-asserted-by":"crossref","first-page":"1219","DOI":"10.1083\/jcb.119.5.1219","volume":"119","year":"1992","journal-title":"J. Cell Biol."},{"key":"2023072213584181700_BIB22","doi-asserted-by":"crossref","first-page":"4466","DOI":"10.1182\/blood.V99.12.4466","volume":"99","year":"2002","journal-title":"Blood."},{"key":"2023072213584181700_BIB23","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1038\/44183","volume":"401","year":"1999","journal-title":"Nature."},{"key":"2023072213584181700_BIB24","doi-asserted-by":"crossref","first-page":"1347","DOI":"10.1016\/S0960-9822(98)00015-3","volume":"8","year":"1998","journal-title":"Curr. Biol."},{"key":"2023072213584181700_BIB25","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1002\/cm.10087","volume":"54","year":"2003","journal-title":"Cell Motil. Cytoskeleton."},{"key":"2023072213584181700_BIB26","doi-asserted-by":"crossref","first-page":"1097","DOI":"10.1083\/jcb.146.5.1097","volume":"146","year":"1999","journal-title":"J. Cell Biol."},{"key":"2023072213584181700_BIB27","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1038\/34208","volume":"391","year":"1998","journal-title":"Nature."},{"key":"2023072213584181700_BIB28","doi-asserted-by":"crossref","first-page":"6181","DOI":"10.1073\/pnas.95.11.6181","volume":"95","year":"1998","journal-title":"Proc. Natl. Acad. Sci. USA."},{"key":"2023072213584181700_BIB29","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1016\/S0091-679X(08)60298-8","volume":"36","year":"1991","journal-title":"Methods Cell Biol."},{"key":"2023072213584181700_BIB30","doi-asserted-by":"crossref","first-page":"2527","DOI":"10.1074\/jbc.272.4.2527","volume":"272","year":"1997","journal-title":"J. Biol. Chem."},{"key":"2023072213584181700_BIB31","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1083\/jcb.200106157","volume":"155","year":"2001","journal-title":"J. Cell Biol."},{"key":"2023072213584181700_BIB32","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1038\/35017011","volume":"2","year":"2000","journal-title":"Nat. Cell Biol."},{"key":"2023072213584181700_BIB33","doi-asserted-by":"crossref","first-page":"612","DOI":"10.1126\/science.1072309","volume":"297","year":"2002","journal-title":"Science."},{"key":"2023072213584181700_BIB34","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1038\/13040","volume":"1","year":"1999","journal-title":"Nat. Cell Biol."},{"key":"2023072213584181700_BIB35","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1038\/ncb719","volume":"4","year":"2002","journal-title":"Nat. Cell Biol."},{"key":"2023072213584181700_BIB36","doi-asserted-by":"crossref","first-page":"626","DOI":"10.1038\/ncb834","volume":"4","year":"2002","journal-title":"Nat. Cell Biol."},{"key":"2023072213584181700_BIB37","doi-asserted-by":"crossref","first-page":"1519","DOI":"10.1083\/jcb.142.6.1519","volume":"142","year":"1998","journal-title":"J. Cell Biol."},{"key":"2023072213584181700_BIB38","doi-asserted-by":"crossref","first-page":"638","DOI":"10.1038\/272638a0","volume":"272","year":"1978","journal-title":"Nature."},{"key":"2023072213584181700_BIB39","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1006\/jsbi.1994.1043","volume":"113","year":"1994","journal-title":"J. Struct. Biol."},{"key":"2023072213584181700_BIB40","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1016\/S0962-8924(01)02237-1","volume":"12","year":"2002","journal-title":"Trends Cell Biol."},{"key":"2023072213584181700_BIB41","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1023\/A:1005329114263","volume":"19","year":"1998","journal-title":"J. Muscle Res. Cell Motil"},{"key":"2023072213584181700_BIB42","doi-asserted-by":"crossref","first-page":"4866","DOI":"10.1016\/S0021-9258(18)62016-2","volume":"246","year":"1971","journal-title":"J. Biol. Chem."},{"key":"2023072213584181700_BIB43","doi-asserted-by":"crossref","first-page":"570","DOI":"10.1016\/S0076-6879(98)98045-4","volume":"298","year":"1998","journal-title":"Methods Enzymol."},{"key":"2023072213584181700_BIB44","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.1083\/jcb.145.5.1009","volume":"145","year":"1999","journal-title":"J. Cell Biol."},{"key":"2023072213584181700_BIB45","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1083\/jcb.139.2.397","volume":"139","year":"1997","journal-title":"J. Cell Biol."},{"key":"2023072213584181700_BIB46","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1083\/jcb.200210174","volume":"160","year":"2003","journal-title":"J. Cell Biol."},{"key":"2023072213584181700_BIB47","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1016\/0092-8674(94)90114-7","volume":"76","year":"1994","journal-title":"Cell."},{"key":"2023072213584181700_BIB48","doi-asserted-by":"crossref","first-page":"993","DOI":"10.1091\/mbc.9.5.993","volume":"9","year":"1998","journal-title":"Mol. Biol. Cell."},{"key":"2023072213584181700_BIB49","doi-asserted-by":"crossref","first-page":"5087","DOI":"10.1016\/S0021-9258(18)89183-9","volume":"260","year":"1985","journal-title":"J. Biol. Chem."},{"key":"2023072213584181700_BIB50","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1016\/S0960-9822(99)80243-7","volume":"9","year":"1999","journal-title":"Curr. Biol."},{"key":"2023072213584181700_BIB51","doi-asserted-by":"crossref","first-page":"1903","DOI":"10.1016\/S0960-9822(01)00603-0","volume":"11","year":"2001","journal-title":"Curr. Biol."}],"container-title":["The Journal of Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/160\/6\/951\/1520991\/jcb1606951.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/160\/6\/951\/1520991\/jcb1606951.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,22]],"date-time":"2023-07-22T09:59:17Z","timestamp":1690019957000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jcb\/article\/160\/6\/951\/33021\/Formation-of-filopodia-like-bundles-in-vitro-from"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,3,17]]},"references-count":51,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2003,3,17]]}},"URL":"https:\/\/doi.org\/10.1083\/jcb.200208059","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.718518499.793509853","asserted-by":"object"}]},"ISSN":["1540-8140","0021-9525"],"issn-type":[{"value":"1540-8140","type":"electronic"},{"value":"0021-9525","type":"print"}],"subject":[],"published":{"date-parts":[[2003,3,17]]}}}