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We show that in the absence of TgNHE1, Toxoplasma is resistant to ionophore-induced egress and extracellular death and amiloride-induced proton efflux inhibition. In addition, the mutant has increased levels of intracellular Ca2+, which explains its decreased sensitivity to A23187. These results provide direct genetic evidence of a role for NHE1 in Ca2+ homeostasis and important insight into how this ubiquitous pathogen senses and responds to changes in its environment.<\/jats:p>","DOI":"10.1083\/jcb.200309097","type":"journal-article","created":{"date-parts":[[2004,6,7]],"date-time":"2004-06-07T19:46:20Z","timestamp":1086637580000},"page":"653-662","update-policy":"http:\/\/dx.doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":71,"title":["Ionophore-resistant mutant of <i>Toxoplasma gondii<\/i> reveals involvement of a sodium\/hydrogen exchanger in calcium regulation"],"prefix":"10.1083","volume":"165","author":[{"given":"Gustavo","family":"Arrizabalaga","sequence":"first","affiliation":[{"name":"1Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305"}]},{"given":"Felix","family":"Ruiz","sequence":"additional","affiliation":[{"name":"2Laboratory of Molecular Parasitology and Center for Zoonoses Research, Department of Pathobiology, University of Illinois at Urbana-Champaign, Urbana, IL 61802"}]},{"given":"Silvia","family":"Moreno","sequence":"additional","affiliation":[{"name":"2Laboratory of Molecular Parasitology and Center for Zoonoses Research, Department of Pathobiology, University of Illinois at Urbana-Champaign, Urbana, IL 61802"}]},{"given":"John C.","family":"Boothroyd","sequence":"additional","affiliation":[{"name":"1Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305"}]}],"member":"291","published-online":{"date-parts":[[2004,6,1]]},"reference":[{"key":"2023072215072806800_BIB1","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1016\/0898-6568(91)90075-6","volume":"3","year":"1991","journal-title":"Cell. 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