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Here, we show that membrane insertion of PA already occurs in early endosomes, possibly only in the multivesicular regions, but that subsequent delivery of LF to the cytoplasm occurs preferentially later in the endocytic pathway and relies on the dynamics of internal vesicles of multivesicular late endosomes.<\/jats:p>","DOI":"10.1083\/jcb.200312072","type":"journal-article","created":{"date-parts":[[2004,8,30]],"date-time":"2004-08-30T14:33:10Z","timestamp":1093876390000},"page":"645-651","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":179,"title":["Membrane insertion of anthrax protective antigen and cytoplasmic delivery of lethal factor occur at different stages of the endocytic pathway"],"prefix":"10.1083","volume":"166","author":[{"given":"Laurence","family":"Abrami","sequence":"first","affiliation":[{"name":"1Department of Microbiology and Molecular Medicine, University of Geneva, Geneva, Switzerland 1211"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margaret","family":"Lindsay","sequence":"additional","affiliation":[{"name":"2Institute for Molecular Bioscience, Centre for Microscopy and Microanalysis, and Department of Physiology and Pharmacology, University of Queensland, Brisbane, Australia 4072"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert G.","family":"Parton","sequence":"additional","affiliation":[{"name":"2Institute for Molecular Bioscience, Centre for Microscopy and Microanalysis, and Department of Physiology and Pharmacology, University of Queensland, Brisbane, Australia 4072"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen H.","family":"Leppla","sequence":"additional","affiliation":[{"name":"3Microbial Pathogenesis Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F. 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