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A combination of time-lapse microscopy and photobleaching techniques reveal that in the absence of palmitoylation, GFP-tagged HRas and NRas undergo rapid exchange between the cytosol and ER\/Golgi membranes, and that wild-type GFP-HRas and GFP-NRas are recycled to the Golgi complex by a nonvesicular mechanism. Our findings support a model where palmitoylation kinetically traps Ras on membranes, enabling the protein to undergo vesicular transport. We propose that a cycle of depalmitoylation and repalmitoylation regulates the time course and sites of Ras signaling by allowing the protein to be released from the cell surface and rapidly redistributed to intracellular membranes.<\/jats:p>","DOI":"10.1083\/jcb.200502063","type":"journal-article","created":{"date-parts":[[2005,7,18]],"date-time":"2005-07-18T18:38:15Z","timestamp":1121711895000},"page":"261-272","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":257,"title":["Depalmitoylated Ras traffics to and from the Golgi complex via a nonvesicular pathway"],"prefix":"10.1083","volume":"170","author":[{"given":"J. Shawn","family":"Goodwin","sequence":"first","affiliation":[{"name":"1Department of Molecular Physiology and Biophysics and Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN 37232"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kimberly R.","family":"Drake","sequence":"additional","affiliation":[{"name":"1Department of Molecular Physiology and Biophysics and Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN 37232"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carl","family":"Rogers","sequence":"additional","affiliation":[{"name":"1Department of Molecular Physiology and Biophysics and Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN 37232"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Latasha","family":"Wright","sequence":"additional","affiliation":[{"name":"3Department of Cell Biology, New York University School of Medicine, New York, NY 10016"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer","family":"Lippincott-Schwartz","sequence":"additional","affiliation":[{"name":"2Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 21218"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark R.","family":"Philips","sequence":"additional","affiliation":[{"name":"3Department of Cell Biology, New York University School of Medicine, New York, NY 10016"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne K.","family":"Kenworthy","sequence":"additional","affiliation":[{"name":"1Department of Molecular Physiology and Biophysics and Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN 37232"},{"name":"2Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 21218"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[2005,7,18]]},"reference":[{"key":"2023072217344080000_BIB1","doi-asserted-by":"crossref","first-page":"2475","DOI":"10.1128\/MCB.20.7.2475-2487.2000","volume":"20","year":"2000","journal-title":"Mol. 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