{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T03:12:07Z","timestamp":1773717127489,"version":"3.50.1"},"reference-count":48,"publisher":"Rockefeller University Press","issue":"4","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2007,2,12]]},"abstract":"<jats:p>The small GTPase Rab7 controls late endocytic transport by the minus end\u2013directed motor protein complex dynein\u2013dynactin, but how it does this is unclear. Rab7-interacting lysosomal protein (RILP) and oxysterol-binding protein\u2013related protein 1L (ORP1L) are two effectors of Rab7. We show that GTP-bound Rab7 simultaneously binds RILP and ORP1L to form a RILP\u2013Rab7\u2013ORP1L complex. RILP interacts directly with the C-terminal 25-kD region of the dynactin projecting arm p150Glued, which is required for dynein motor recruitment to late endocytic compartments (LEs). Still, p150Glued recruitment by Rab7\u2013RILP does not suffice to induce dynein-driven minus-end transport of LEs. ORP1L, as well as \u03b2III spectrin, which is the general receptor for dynactin on vesicles, are essential for dynein motor activity. Our results illustrate that the assembly of microtubule motors on endosomes involves a cascade of linked events. First, Rab7 recruits two effectors, RILP and ORP1L, to form a tripartite complex. Next, RILP directly binds to the p150Glued dynactin subunit to recruit the dynein motor. Finally, the specific dynein motor receptor Rab7\u2013RILP is transferred by ORP1L to \u03b2III spectrin. Dynein will initiate translocation of late endosomes to microtubule minus ends only after interacting with \u03b2III spectrin, which requires the activities of Rab7\u2013RILP and ORP1L.<\/jats:p>","DOI":"10.1083\/jcb.200606077","type":"journal-article","created":{"date-parts":[[2007,2,6]],"date-time":"2007-02-06T01:36:15Z","timestamp":1170725775000},"page":"459-471","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":420,"title":["Activation of endosomal dynein motors by stepwise assembly of Rab7\u2013RILP\u2013p150Glued, ORP1L, and the receptor \u03b2lll spectrin"],"prefix":"10.1083","volume":"176","author":[{"given":"Marie","family":"Johansson","sequence":"first","affiliation":[{"name":"1Department of Molecular Medicine, National Public Health Institute, Biomedicum, FI-00251 Helsinki, Finland"}]},{"given":"Nuno","family":"Rocha","sequence":"additional","affiliation":[{"name":"2Division of Tumor Biology, The Netherlands Cancer Institute, 1066CX Amsterdam, Netherlands"}]},{"given":"Wilbert","family":"Zwart","sequence":"additional","affiliation":[{"name":"2Division of Tumor Biology, The Netherlands Cancer Institute, 1066CX Amsterdam, Netherlands"}]},{"given":"Ingrid","family":"Jordens","sequence":"additional","affiliation":[{"name":"2Division of Tumor Biology, The Netherlands Cancer Institute, 1066CX Amsterdam, Netherlands"}]},{"given":"Lennert","family":"Janssen","sequence":"additional","affiliation":[{"name":"2Division of Tumor Biology, The Netherlands Cancer Institute, 1066CX Amsterdam, Netherlands"}]},{"given":"Coenraad","family":"Kuijl","sequence":"additional","affiliation":[{"name":"2Division of Tumor Biology, The Netherlands Cancer Institute, 1066CX Amsterdam, Netherlands"}]},{"given":"Vesa M.","family":"Olkkonen","sequence":"additional","affiliation":[{"name":"1Department of Molecular Medicine, National Public Health Institute, Biomedicum, FI-00251 Helsinki, Finland"}]},{"given":"Jacques","family":"Neefjes","sequence":"additional","affiliation":[{"name":"2Division of Tumor Biology, The Netherlands Cancer Institute, 1066CX Amsterdam, Netherlands"}]}],"member":"291","published-online":{"date-parts":[[2007,2,5]]},"reference":[{"key":"2023072908154979900_BIB1","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1083\/jcb.140.2.391","volume":"140","year":"1998","journal-title":"J. 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