{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T20:28:27Z","timestamp":1772137707631,"version":"3.50.1"},"reference-count":29,"publisher":"Rockefeller University Press","issue":"2","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2007,7,16]]},"abstract":"<jats:p>Phosphoinositide 3-kinase (PI3K)\u03b3 and Dictyostelium PI3K are activated via G protein\u2013coupled receptors through binding to the G\u03b2\u03b3 subunit and Ras. However, the mechanistic role(s) of G\u03b2\u03b3 and Ras in PI3K activation remains elusive. Furthermore, the dynamics and function of PI3K activation in the absence of extracellular stimuli have not been fully investigated. We report that g\u03b2 null cells display PI3K and Ras activation, as well as the reciprocal localization of PI3K and PTEN, which lead to local accumulation of PI(3,4,5)P3. Simultaneous imaging analysis reveals that in the absence of extracellular stimuli, autonomous PI3K and Ras activation occur, concurrently, at the same sites where F-actin projection emerges. The loss of PI3K binding to Ras\u2013guanosine triphosphate abolishes this PI3K activation, whereas prevention of PI3K activity suppresses autonomous Ras activation, suggesting that PI3K and Ras form a positive feedback circuit. This circuit is associated with both random cell migration and cytokinesis and may have initially evolved to control stochastic changes in the cytoskeleton.<\/jats:p>","DOI":"10.1083\/jcb.200611138","type":"journal-article","created":{"date-parts":[[2007,7,16]],"date-time":"2007-07-16T14:08:13Z","timestamp":1184594893000},"page":"185-191","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":211,"title":["G protein\u2013independent Ras\/PI3K\/F-actin circuit regulates basic cell motility"],"prefix":"10.1083","volume":"178","author":[{"given":"Atsuo T.","family":"Sasaki","sequence":"first","affiliation":[{"name":"1Section of Cell and Developmental Biology, Division of Biological Sciences, and Center for Molecular Genetics, University of California, San Diego, La Jolla, CA 92093"},{"name":"2Department of Systems Biology and Division of Signal Transduction, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02115"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chris","family":"Janetopoulos","sequence":"additional","affiliation":[{"name":"3Department of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, MD 21205"},{"name":"4Department of Biological Sciences, Vanderbilt University, Nashville, TN 37235"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan","family":"Lee","sequence":"additional","affiliation":[{"name":"1Section of Cell and Developmental Biology, Division of Biological Sciences, and Center for Molecular Genetics, University of California, San Diego, La Jolla, CA 92093"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pascale G.","family":"Charest","sequence":"additional","affiliation":[{"name":"1Section of Cell and Developmental Biology, Division of Biological Sciences, and Center for Molecular Genetics, University of California, San Diego, La Jolla, CA 92093"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kosuke","family":"Takeda","sequence":"additional","affiliation":[{"name":"1Section of Cell and Developmental Biology, Division of Biological Sciences, and Center for Molecular Genetics, University of California, San Diego, La Jolla, CA 92093"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lauren W.","family":"Sundheimer","sequence":"additional","affiliation":[{"name":"1Section of Cell and Developmental Biology, Division of Biological Sciences, and Center for Molecular Genetics, University of California, San Diego, La Jolla, CA 92093"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruedi","family":"Meili","sequence":"additional","affiliation":[{"name":"1Section of Cell and Developmental Biology, Division of Biological Sciences, and Center for Molecular Genetics, University of California, San Diego, La Jolla, CA 92093"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter N.","family":"Devreotes","sequence":"additional","affiliation":[{"name":"4Department of Biological Sciences, Vanderbilt University, Nashville, TN 37235"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard A.","family":"Firtel","sequence":"additional","affiliation":[{"name":"1Section of Cell and Developmental Biology, Division of Biological Sciences, and Center for Molecular Genetics, University of California, San Diego, La Jolla, CA 92093"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[2007,7,16]]},"reference":[{"key":"2023072909013406600_BIB1","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1083\/jcb.200210115","volume":"160","year":"2003","journal-title":"J. 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