{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T22:30:18Z","timestamp":1772577018442,"version":"3.50.1"},"reference-count":28,"publisher":"Rockefeller University Press","issue":"3","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2008,2,11]]},"abstract":"<jats:p>Lipid droplets (LDs) are emerging cellular organelles that are of crucial importance in cell biology and human diseases. In this study, we present our screen of \u223c4,700 Saccharomyces cerevisiae mutants for abnormalities in the number and morphology of LDs; we identify 17 fld (few LDs) and 116 mld (many LDs) mutants. One of the fld mutants (fld1) is caused by the deletion of YLR404W, a previously uncharacterized open reading frame. Cells lacking FLD1 contain strikingly enlarged (supersized) LDs, and LDs from fld1\u0394 cells demonstrate significantly enhanced fusion activities both in vivo and in vitro. Interestingly, the expression of human seipin, whose mutant forms are associated with Berardinelli-Seip congenital lipodystrophy and motoneuron disorders, rescues LD-associated defects in fld1\u0394 cells. Lipid profiling reveals alterations in acyl chain compositions of major phospholipids in fld1\u0394 cells. These results suggest that an evolutionally conserved function of seipin in phospholipid metabolism and LD formation may be functionally important in human adipogenesis.<\/jats:p>","DOI":"10.1083\/jcb.200711136","type":"journal-article","created":{"date-parts":[[2008,2,5]],"date-time":"2008-02-05T01:47:42Z","timestamp":1202176062000},"page":"473-482","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":440,"title":["Fld1p, a functional homologue of human seipin, regulates the size of lipid droplets in yeast"],"prefix":"10.1083","volume":"180","author":[{"given":"Weihua","family":"Fei","sequence":"first","affiliation":[{"name":"1Department of Biochemistry, National University of Singapore, Singapore 117597, Republic of Singapore"}]},{"given":"Guanghou","family":"Shui","sequence":"additional","affiliation":[{"name":"1Department of Biochemistry, National University of Singapore, Singapore 117597, Republic of Singapore"}]},{"given":"Bruno","family":"Gaeta","sequence":"additional","affiliation":[{"name":"2School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney 2052, New South Wales, Australia"}]},{"given":"Ximing","family":"Du","sequence":"additional","affiliation":[{"name":"2School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney 2052, New South Wales, Australia"}]},{"given":"Lars","family":"Kuerschner","sequence":"additional","affiliation":[{"name":"3Institute for Molecular Bioscience"},{"name":"4Centre for Microscopy and Microanalysis, University of Queensland, Brisbane 4072, Australia"}]},{"given":"Peng","family":"Li","sequence":"additional","affiliation":[{"name":"5Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China"}]},{"given":"Andrew J.","family":"Brown","sequence":"additional","affiliation":[{"name":"2School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney 2052, New South Wales, Australia"}]},{"given":"Markus R.","family":"Wenk","sequence":"additional","affiliation":[{"name":"1Department of Biochemistry, National University of Singapore, Singapore 117597, Republic of Singapore"}]},{"given":"Robert G.","family":"Parton","sequence":"additional","affiliation":[{"name":"3Institute for Molecular Bioscience"},{"name":"4Centre for Microscopy and Microanalysis, University of Queensland, Brisbane 4072, Australia"}]},{"given":"Hongyuan","family":"Yang","sequence":"additional","affiliation":[{"name":"1Department of Biochemistry, National University of Singapore, Singapore 117597, Republic of Singapore"},{"name":"2School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney 2052, New South Wales, Australia"}]}],"member":"291","published-online":{"date-parts":[[2008,2,4]]},"reference":[{"key":"2023072223135762400_BIB1","doi-asserted-by":"crossref","first-page":"440","DOI":"10.1016\/j.molmed.2004.07.009","volume":"10","year":"2004","journal-title":"Trends Mol. 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