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Clathrin colocalized with a subset of focal adhesions in an ARH- and Dab2-dependent fashion. Direct imaging showed that clathrin rapidly accumulated on focal adhesions during MT-stimulated disassembly and departed from focal adhesions with integrin upon their disassembly. In migrating cells, depletion of clathrin or Dab2 and ARH inhibited focal adhesion disassembly and decreased the rate of migration. These results show that focal adhesion disassembly occurs through a targeted mechanism involving MTs, clathrin, and specific clathrin adaptors and that direct endocytosis of integrins from focal adhesions mediates their disassembly in migrating cells.<\/jats:p>","DOI":"10.1083\/jcb.200904054","type":"journal-article","created":{"date-parts":[[2009,11,30]],"date-time":"2009-11-30T18:51:09Z","timestamp":1259607069000},"page":"733-747","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":308,"title":["Clathrin mediates integrin endocytosis for focal adhesion disassembly in migrating cells"],"prefix":"10.1083","volume":"187","author":[{"given":"Ellen J.","family":"Ezratty","sequence":"first","affiliation":[{"name":"Department of Pathology and Cell Biology, Columbia University, New York, NY 10032"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claire","family":"Bertaux","sequence":"additional","affiliation":[{"name":"Department of Pathology and Cell Biology, Columbia University, New York, NY 10032"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eugene E.","family":"Marcantonio","sequence":"additional","affiliation":[{"name":"Department of Pathology and Cell Biology, Columbia University, New York, NY 10032"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gregg G.","family":"Gundersen","sequence":"additional","affiliation":[{"name":"Department of Pathology and Cell Biology, Columbia University, New York, NY 10032"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[2009,11,30]]},"reference":[{"key":"2023072914310293300_bib1","doi-asserted-by":"publisher","first-page":"129","DOI":"10.1098\/rspb.1980.0017","article-title":"The crawling behavior of metazoan cells","volume":"207","author":"Abercrombie","year":"1980","journal-title":"Proc. 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