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In this study, we use a biosensor specific for cyclin B1\u2013Cdk1 activity to show that activating cyclin B1\u2013Cdk1 immediately triggers its rapid accumulation in the nucleus through a 40-fold increase in nuclear import that remains dependent on Cdk1 activity until NEBD. Nevertheless, a substantial proportion of cyclin B1\u2013Cdk1 remains in the cytoplasm. The increase in nuclear import is driven by changes in the nuclear import machinery that require neither Plk1 nor inhibition of nuclear export. Thus, the intrinsic link between cyclin B1\u2013Cdk1 activation and its rapid nuclear import inherently coordinates the reorganization of the nucleus and the cytoplasm at mitotic entry.<\/jats:p>","DOI":"10.1083\/jcb.200909144","type":"journal-article","created":{"date-parts":[[2010,4,19]],"date-time":"2010-04-19T17:58:36Z","timestamp":1271699916000},"page":"247-259","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":281,"title":["Activation of cyclin B1\u2013Cdk1 synchronizes events in the nucleus and the cytoplasm at mitosis"],"prefix":"10.1083","volume":"189","author":[{"given":"Olivier","family":"Gavet","sequence":"first","affiliation":[{"name":"The Wellcome Trust\/Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge CB2 1QN, England, UK"}]},{"given":"Jonathon","family":"Pines","sequence":"additional","affiliation":[{"name":"The Wellcome Trust\/Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge CB2 1QN, England, UK"}]}],"member":"291","published-online":{"date-parts":[[2010,4,19]]},"reference":[{"key":"2023072223513683700_bib1","doi-asserted-by":"publisher","first-page":"301","DOI":"10.1016\/j.molcel.2007.03.019","article-title":"Phosphorylation of caspase-9 by CDK1\/cyclin B1 protects mitotic cells against apoptosis","volume":"26","author":"Allan","year":"2007","journal-title":"Mol. 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