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OATL1 is recruited to isolation membranes and autophagosomes through direct interaction with Atg8 homologues and is involved in the fusion between autophagosomes and lysosomes through its GAP activity. We further provide evidence that Rab33B, an Atg16L1-binding protein, is a target substrate of OATL1 and is involved in the fusion between autophagosomes and lysosomes, the same as OATL1. Because both its GAP activity and its Atg8 homologue\u2013binding activity are required for OATL1 to function, we propose a model that OATL1 uses Atg8 homologues as a scaffold to exert its GAP activity and to regulate autophagosomal maturation.<\/jats:p>","DOI":"10.1083\/jcb.201008107","type":"journal-article","created":{"date-parts":[[2011,3,8]],"date-time":"2011-03-08T11:03:11Z","timestamp":1299582191000},"page":"839-853","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":148,"title":["OATL1, a novel autophagosome-resident Rab33B-GAP, regulates autophagosomal maturation"],"prefix":"10.1083","volume":"192","author":[{"given":"Takashi","family":"Itoh","sequence":"first","affiliation":[{"name":"Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku University, Aobayama, Aoba-ku, Sendai, Miyagi 980-8578, Japan 1"}]},{"given":"Eiko","family":"Kanno","sequence":"additional","affiliation":[{"name":"Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku University, Aobayama, Aoba-ku, Sendai, Miyagi 980-8578, Japan 1"}]},{"given":"Takefumi","family":"Uemura","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Histology, Fukushima Medical University School of Medicine, Fukushima 960-1295, Japan 2"}]},{"given":"Satoshi","family":"Waguri","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Histology, Fukushima Medical University School of Medicine, Fukushima 960-1295, Japan 2"}]},{"given":"Mitsunori","family":"Fukuda","sequence":"additional","affiliation":[{"name":"Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku University, Aobayama, Aoba-ku, Sendai, Miyagi 980-8578, Japan 1"}]}],"member":"291","published-online":{"date-parts":[[2011,3,7]]},"reference":[{"key":"2023072905191770100_bib1","doi-asserted-by":"publisher","first-page":"68","DOI":"10.1038\/nature09204","article-title":"Network organization of the human autophagy system","volume":"466","author":"Behrends","year":"2010","journal-title":"Nature."},{"key":"2023072905191770100_bib2","first-page":"47","article-title":"GAPs galore! 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