{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,30]],"date-time":"2025-09-30T11:01:47Z","timestamp":1759230107397},"reference-count":0,"publisher":"Rockefeller University Press","issue":"5","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1988,11,1]]},"abstract":"<jats:p>The binding and transport of glycoalbumin (gA) by the endothelium of murine myocardial microvessels were studied by perfusing in situ 125I-gA or gA-gold complexes (gA-Au) and examining the specimens by radioassays and EM, respectively. After a 3-min perfusion, the uptake of radioiodinated gA is 2.2-fold higher than that of native albumin; it is partially (approximately 55%) competed by either albumin or D-glucose, and almost completely abolished by the concomitant administration of both competitors or by gA. D-mannose and D-galactose are not effective competitors. Unlike albumin-gold complexes that bind restrictively to plasmalemmal vesicles, gA-Au labels the plasma-lemma proper, plasmalemmal vesicles open on the lumen, and most coated pits. Competing albumin prevents gA-Au binding to the membrane of plasmalemmal vesicles, while glucose significantly reduces the ligand binding to plasmalemma proper. Competition with albumin and glucose gives additive effects. Transcytosis of gA-Au, already detected at 3 min, becomes substantial by 30 min. No tracer exit via intercellular junctions was detected. gA-Au progressively accumulates in multivesicular bodies. The results of the binding and competition experiments indicate that the gA behaves as a bifunctional ligand which is recognized by two distinct binding sites: one, located on the plasma membrane, binds as a lectin the glucose residues of gA; whereas the other, confined to plasmalemmal vesicles, recognizes presumably specific domains of the albumin molecule.<\/jats:p>","DOI":"10.1083\/jcb.107.5.1729","type":"journal-article","created":{"date-parts":[[2004,5,15]],"date-time":"2004-05-15T00:18:20Z","timestamp":1084580300000},"page":"1729-1738","update-policy":"http:\/\/dx.doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":44,"title":["Binding and transcytosis of glycoalbumin by the microvascular endothelium of the murine myocardium: evidence that glycoalbumin behaves as a bifunctional ligand."],"prefix":"10.1083","volume":"107","author":[{"given":"D","family":"Predescu","sequence":"first","affiliation":[{"name":"Institute of Cellular Biology and Pathology, Bucharest, Romania."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Simionescu","sequence":"additional","affiliation":[{"name":"Institute of Cellular Biology and Pathology, Bucharest, Romania."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N","family":"Simionescu","sequence":"additional","affiliation":[{"name":"Institute of Cellular Biology and Pathology, Bucharest, Romania."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G E","family":"Palade","sequence":"additional","affiliation":[{"name":"Institute of Cellular Biology and Pathology, Bucharest, Romania."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[1988,11,1]]},"container-title":["The Journal of cell biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/107\/5\/1729\/1461798\/1729.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/107\/5\/1729\/1461798\/1729.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,22]],"date-time":"2023-07-22T00:41:21Z","timestamp":1689986481000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jcb\/article\/107\/5\/1729\/55494\/Binding-and-transcytosis-of-glycoalbumin-by-the"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1988,11,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1988,11,1]]}},"URL":"https:\/\/doi.org\/10.1083\/jcb.107.5.1729","relation":{},"ISSN":["0021-9525","1540-8140"],"issn-type":[{"value":"0021-9525","type":"print"},{"value":"1540-8140","type":"electronic"}],"subject":[],"published":{"date-parts":[[1988,11,1]]}}}