{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T14:03:23Z","timestamp":1773324203757,"version":"3.50.1"},"reference-count":0,"publisher":"Rockefeller University Press","issue":"6","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1989,6,1]]},"abstract":"<jats:p>A clonal strain of human lung tumor cells in culture (ChaGo), derived from a bronchogenic carcinoma, synthesizes and secretes large amounts of alpha (alpha) and a comparatively lower level of beta (beta) subunit of the glycoprotein hormone, human chorionic gonadotropin (HCG). ChaGo cells lost their characteristic anchorage-independent growth phenotype in the presence of anti-alpha-HCG antibody. The effect of the antibody was partially reversed by addition of alpha-HCG to the culture medium. ChaGo cells were transfected with an expression vector (pRSV-anti-alpha-HCG), that directs synthesis of RNA complementary to alpha-HCG mRNA. The transfectants produced alpha-HCG antisense RNA which was associated with the reduced level of alpha-HCG. Transfectants also displayed several altered phenotypic properties, including altered morphology, less mitosis, reduced growth rate, loss of anchorage-independent growth, and loss of tumorigenicity in nude mice. Treatment of transfectants with 8,bromo-cAMP resulted in increased accumulation of alpha-HCG mRNA, no change in the level of alpha-HCG antisense RNA, release of the inhibition of [3H]thymidine incorporation, and restoration of anchorage-independent growth phenotype. The overexpression of c-myc, observed in ChaGo cells, was unaffected by the reduced level of alpha-HCG. These results suggest that ectopic synthesis of the alpha subunit of HCG plays a functional role in the transformation of these human lung cells.<\/jats:p>","DOI":"10.1083\/jcb.108.6.2423","type":"journal-article","created":{"date-parts":[[2004,5,15]],"date-time":"2004-05-15T00:18:20Z","timestamp":1084580300000},"page":"2423-2434","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":36,"title":["Loss of tumorigenic potential by human lung tumor cells in the presence of antisense RNA specific to the ectopically synthesized alpha subunit of human chorionic gonadotropin."],"prefix":"10.1083","volume":"108","author":[{"given":"R T","family":"Rivera","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Biology, Harvard School of Dental Medicine, Boston, Massachusetts 02115."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S G","family":"Pasion","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Biology, Harvard School of Dental Medicine, Boston, Massachusetts 02115."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D T","family":"Wong","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Biology, Harvard School of Dental Medicine, Boston, Massachusetts 02115."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y B","family":"Fei","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Biology, Harvard School of Dental Medicine, Boston, Massachusetts 02115."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D K","family":"Biswas","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Biology, Harvard School of Dental Medicine, Boston, Massachusetts 02115."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[1989,6,1]]},"container-title":["The Journal of cell biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/108\/6\/2423\/1462470\/2423.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/108\/6\/2423\/1462470\/2423.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,22]],"date-time":"2023-07-22T00:51:24Z","timestamp":1689987084000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jcb\/article\/108\/6\/2423\/55644\/Loss-of-tumorigenic-potential-by-human-lung-tumor"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,6,1]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1989,6,1]]}},"URL":"https:\/\/doi.org\/10.1083\/jcb.108.6.2423","relation":{},"ISSN":["0021-9525","1540-8140"],"issn-type":[{"value":"0021-9525","type":"print"},{"value":"1540-8140","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,6,1]]}}}