{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T06:03:05Z","timestamp":1779343385441,"version":"3.51.4"},"reference-count":0,"publisher":"Rockefeller University Press","issue":"1","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1989,7,1]]},"abstract":"<jats:p>We have investigated the sorting and packaging of secretory proteins into secretory granules by an immunological approach. An mAb against secretogranin I (chromogranin B), a secretory protein costored with various peptide hormones and neuropeptides in secretory granules of many endocrine cells and neurons, was expressed by microinjection of its mRNA into the secretogranin I-producing cell line PC12. An mAb against the G protein of vesicular stomatitis virus--i.e., against an antigen not present in PC12 cells--was expressed as a control. The intracellular localization and the secretion of the antibodies was studied by double-labeling immunofluorescence using the conventional and the confocal microscope, as well as by pulse-chase experiments. The secretogranin I antibody, like the control antibody, was transported along the secretory pathway to the Golgi complex. However, in contrast to the control antibody, which was secreted via the constitutive pathway, the secretogranin I antibody formed an immunocomplex with secretogranin I, was packaged into secretory granules, and was released by regulated exocytosis. Our results show that a constitutive secretory protein, unaltered by genetic engineering, can be diverted to the regulated pathway of secretion by its protein-protein interaction with a regulated secretory protein. The data also provide the basis for immunologically studying the role of luminally exposed protein domains in the biogenesis and function of regulated secretory vesicles.<\/jats:p>","DOI":"10.1083\/jcb.109.1.17","type":"journal-article","created":{"date-parts":[[2004,5,15]],"date-time":"2004-05-15T00:18:20Z","timestamp":1084580300000},"page":"17-34","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":82,"title":["An antibody against secretogranin I (chromogranin B) is packaged into secretory granules."],"prefix":"10.1083","volume":"109","author":[{"given":"P","family":"Rosa","sequence":"first","affiliation":[{"name":"Cell Biology Programme, European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"U","family":"Weiss","sequence":"additional","affiliation":[{"name":"Cell Biology Programme, European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"Pepperkok","sequence":"additional","affiliation":[{"name":"Cell Biology Programme, European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W","family":"Ansorge","sequence":"additional","affiliation":[{"name":"Cell Biology Programme, European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Niehrs","sequence":"additional","affiliation":[{"name":"Cell Biology Programme, European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E H","family":"Stelzer","sequence":"additional","affiliation":[{"name":"Cell Biology Programme, European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W B","family":"Huttner","sequence":"additional","affiliation":[{"name":"Cell Biology Programme, European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[1989,7,1]]},"container-title":["The Journal of cell biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/109\/1\/17\/1462393\/17.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/109\/1\/17\/1462393\/17.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,22]],"date-time":"2023-07-22T00:50:30Z","timestamp":1689987030000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jcb\/article\/109\/1\/17\/59262\/An-antibody-against-secretogranin-I-chromogranin-B"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,7,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1989,7,1]]}},"URL":"https:\/\/doi.org\/10.1083\/jcb.109.1.17","relation":{},"ISSN":["0021-9525","1540-8140"],"issn-type":[{"value":"0021-9525","type":"print"},{"value":"1540-8140","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,7,1]]}}}