{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T13:12:04Z","timestamp":1790514724909,"version":"4.1.0"},"reference-count":0,"publisher":"Rockefeller University Press","issue":"4","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1989,10,1]]},"abstract":"<jats:p>P-cadherin is a subclass of Ca2+-dependent cell-cell adhesion molecules present in mouse placenta, where its localization suggests a function of connecting the embryo to the uterus (Nose, A., and M. Takeichi. 1986. J. Cell Biol. 103:2649-2658). We recently identified a human cadherin detected by an mAb capable of disrupting cell-cell adhesion of A-431 cells, and found that it was closely related immunochemically to mouse P-cadherin. Curiously, this cadherin was undetectable in human placenta by immunohistochemical examination (Shimoyama, Y., S. Hirohashi, S. Hirano, M. Noguchi, Y. Shimosato, M. Takeichi, and O. Abe. 1989. Cancer Res. 49:2128-2133). We here report the cloning and sequencing of cDNA clone encoding the human homologue of mouse P-cadherin. The deduced amino acid sequence of the human P-cadherin consists of 829 amino acid and shows striking homology with mouse P-cadherin. On Northern blot analysis, human P-cadherin was scarcely expressed in human placenta in contrast to mouse P-cadherin, which was abundantly expressed in mouse placenta throughout pregnancy, and it was shown that E-cadherin, but not P-cadherin, was the major cadherin molecule in human placenta. Moreover, NIH3T3 cells transfected with human P-cadherin cDNA expressed the functional cadherin molecule, which was identical to the cadherin we had previously identified using the mAb, showing that this molecule really does mediate cell-cell adhesion and that the cadherin we detected immunochemically is undoubtedly human P-cadherin. The results obtained in this study support the idea that P-cadherin plays little role, if any, in Ca2+-dependent cell-cell binding in human placental tissue at least after several weeks of pregnancy.<\/jats:p>","DOI":"10.1083\/jcb.109.4.1787","type":"journal-article","created":{"date-parts":[[2004,5,15]],"date-time":"2004-05-15T00:18:20Z","timestamp":1084580300000},"page":"1787-1794","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":84,"title":["Molecular cloning of a human Ca2+-dependent cell-cell adhesion molecule homologous to mouse placental cadherin: its low expression in human placental tissues."],"prefix":"10.1083","volume":"109","author":[{"given":"Y","family":"Shimoyama","sequence":"first","affiliation":[{"name":"Pathology and Genetics Division, National Cancer Center Research Institute, Tokyo, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Yoshida","sequence":"additional","affiliation":[{"name":"Pathology and Genetics Division, National Cancer Center Research Institute, Tokyo, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Terada","sequence":"additional","affiliation":[{"name":"Pathology and Genetics Division, National Cancer Center Research Institute, Tokyo, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y","family":"Shimosato","sequence":"additional","affiliation":[{"name":"Pathology and Genetics Division, National Cancer Center Research Institute, Tokyo, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O","family":"Abe","sequence":"additional","affiliation":[{"name":"Pathology and Genetics Division, National Cancer Center Research Institute, Tokyo, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Hirohashi","sequence":"additional","affiliation":[{"name":"Pathology and Genetics Division, National Cancer Center Research Institute, Tokyo, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1989,10,1]]},"container-title":["The Journal of cell biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/109\/4\/1787\/1466019\/1787.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/109\/4\/1787\/1466019\/1787.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,22]],"date-time":"2023-07-22T01:40:21Z","timestamp":1689990021000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jcb\/article\/109\/4\/1787\/28700\/Molecular-cloning-of-a-human-Ca2-dependent-cell"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,10,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1989,10,1]]}},"URL":"https:\/\/doi.org\/10.1083\/jcb.109.4.1787","relation":{},"ISSN":["0021-9525","1540-8140"],"issn-type":[{"value":"0021-9525","type":"print"},{"value":"1540-8140","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,10,1]]}}}