{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T23:06:40Z","timestamp":1790896000258,"version":"4.1.0"},"reference-count":0,"publisher":"Rockefeller University Press","issue":"4","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1990,10,1]]},"abstract":"<jats:p>The integrin alpha 6 beta 4 is a heterodimer predominantly expressed by epithelia. While no definite receptor function has yet been assigned to it, this integrin may mediate adhesive and\/or migratory functions of epithelial cells. We have determined the complete primary structure of both the alpha 6 and beta 4 subunits from cDNA clones isolated from pancreatic carcinoma cell line libraries. The deduced amino acid sequence of alpha 6 is homologous to other integrin alpha chains (18-26% identity). Antibodies to an alpha 6 carboxy terminus peptide immunoprecipitated alpha 6 beta 4 complexes from carcinoma cells and alpha 6 beta 1 complexes from platelets, providing further evidence for the association of alpha 6 with more than one beta subunit. The deduced amino acid sequence of beta 4 predicts an extracellular portion homologous to other integrin beta chains, and a unique cytoplasmic domain comprised of greater than 1,000 residues. This agrees with the structures of the beta 4 cDNAs from normal epithelial cells (Suzuki, S., and Y. Naitoh. 1990. EMBO [Eur. Mol. Biol. Organ.] J. 9:757-763; Hogervost, F., I. Kuikman, A. E. G. Kr. von dem Borne, and A. Sonnenberg. 1990. EMBO [Eur. Mol. Biol. Organ.] J. 9:765-770). Compared to these structures, however, the beta 4 cDNAs that we have cloned from carcinoma cells contain extra sequences. One of these is located in the 5'-untranslated region, and may encode regulatory sequences. Another specifies a segment of 70 amino acids in the cytoplasmic tail. Amplification by reverse transcription-polymerase chain reaction of mRNA indicated that multiple forms of beta 4 may exist, possibly due to cell-type specific alternative splicing. The unique structure of beta 4 suggests its involvement in novel cytoskeletal interactions. Consistent with this possibility, alpha 6 beta 4 is mostly concentrated on the basal surface of epithelial cells, but does not colocalize with components of adhesion plaques.<\/jats:p>","DOI":"10.1083\/jcb.111.4.1593","type":"journal-article","created":{"date-parts":[[2004,5,15]],"date-time":"2004-05-15T00:18:20Z","timestamp":1084580300000},"page":"1593-1604","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":236,"title":["Epithelial integrin alpha 6 beta 4: complete primary structure of alpha 6 and variant forms of beta 4."],"prefix":"10.1083","volume":"111","author":[{"given":"R N","family":"Tamura","sequence":"first","affiliation":[{"name":"Department of Immunology, IMM-8, Research Institute of Scripps Clinic, La Jolla, California 92037."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C","family":"Rozzo","sequence":"additional","affiliation":[{"name":"Department of Immunology, IMM-8, Research Institute of Scripps Clinic, La Jolla, California 92037."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L","family":"Starr","sequence":"additional","affiliation":[{"name":"Department of Immunology, IMM-8, Research Institute of Scripps Clinic, La Jolla, California 92037."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J","family":"Chambers","sequence":"additional","affiliation":[{"name":"Department of Immunology, IMM-8, Research Institute of Scripps Clinic, La Jolla, California 92037."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L F","family":"Reichardt","sequence":"additional","affiliation":[{"name":"Department of Immunology, IMM-8, Research Institute of Scripps Clinic, La Jolla, California 92037."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H M","family":"Cooper","sequence":"additional","affiliation":[{"name":"Department of Immunology, IMM-8, Research Institute of Scripps Clinic, La Jolla, California 92037."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V","family":"Quaranta","sequence":"additional","affiliation":[{"name":"Department of Immunology, IMM-8, Research Institute of Scripps Clinic, La Jolla, California 92037."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1990,10,1]]},"container-title":["The Journal of cell biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/111\/4\/1593\/1463788\/1593.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/111\/4\/1593\/1463788\/1593.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,22]],"date-time":"2023-07-22T01:10:08Z","timestamp":1689988208000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jcb\/article\/111\/4\/1593\/55818\/Epithelial-integrin-alpha-6-beta-4-complete"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,10,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1990,10,1]]}},"URL":"https:\/\/doi.org\/10.1083\/jcb.111.4.1593","relation":{},"ISSN":["0021-9525","1540-8140"],"issn-type":[{"value":"0021-9525","type":"print"},{"value":"1540-8140","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,10,1]]}}}