{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T11:30:56Z","timestamp":1770982256190,"version":"3.50.1"},"reference-count":0,"publisher":"Rockefeller University Press","issue":"5","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1991,3,1]]},"abstract":"<jats:p>The neuron-glia cell adhesion molecule (Ng-CAM) mediates both neuron-neuron and neuron-glia adhesion; it is detected on SDS-PAGE as a predominant 135-kD glycoprotein, with minor components of 80, 190, and 210 kD. We have isolated cDNA clones encoding the entire sequence of chicken Ng-CAM. The predicted extracellular region includes six immunoglobulin-like domains followed by five fibronectin-type III repeats, structural features that are characteristic of several neural CAMs of the N-CAM superfamily. The amino acid sequence of chicken Ng-CAM is most similar to that of mouse L1 but the overall identity is only 40% and Ng-CAM contains a short fibronectin-like segment with an RGD sequence that has no counterpart in L1. These findings suggest that Ng-CAM and L1 may not be equivalent molecules in chicken and mouse. The amino-terminal sequences of the 210-, 190-, and 135-kD components of Ng-CAM are all the same as the predicted amino terminus of the molecule, whereas the 80-kD component begins within the third fibronectin repeat. The cDNA sequence is continuous across the junction between the 135- and 80-kD components, and a single 170-kD Ng-CAM polypeptide was isolated from tunicamycin-treated cells. In addition, all cDNA probes hybridized on Northern blots to a 6-kb RNA, and most hybridized to single bands on Southern blots. These results indicate that the Ng-CAM components are derived from a single polypeptide encoded by a single gene, and that the 135- and 80-kD components are generated from the 210\/190-kD species by proteolytic cleavage. The 135-kD component contains most of the extracellular region including all of the immunoglobulin-like domains. It has no transmembrane segment, but it is tightly associated with the membrane. The 80-kD component contains two and a half type III repeats plus the RGD-containing segment, as well as the single transmembrane and cytoplasmic domains. These structural features of Ng-CAM provide a framework for understanding its multiple functions in neuron-neuron interactions, neurite fasciculation, and neuron-glia interactions.<\/jats:p>","DOI":"10.1083\/jcb.112.5.1017","type":"journal-article","created":{"date-parts":[[2004,5,15]],"date-time":"2004-05-15T00:18:20Z","timestamp":1084580300000},"page":"1017-1029","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":112,"title":["Structure of the chicken neuron-glia cell adhesion molecule, Ng-CAM: origin of the polypeptides and relation to the Ig superfamily."],"prefix":"10.1083","volume":"112","author":[{"given":"M P","family":"Burgoon","sequence":"first","affiliation":[{"name":"Rockefeller University, New York 10021."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Grumet","sequence":"additional","affiliation":[{"name":"Rockefeller University, New York 10021."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V","family":"Mauro","sequence":"additional","affiliation":[{"name":"Rockefeller University, New York 10021."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G M","family":"Edelman","sequence":"additional","affiliation":[{"name":"Rockefeller University, New York 10021."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B A","family":"Cunningham","sequence":"additional","affiliation":[{"name":"Rockefeller University, New York 10021."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[1991,3,1]]},"container-title":["The Journal of cell biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/112\/5\/1017\/1464237\/1017.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/112\/5\/1017\/1464237\/1017.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,22]],"date-time":"2023-07-22T01:16:39Z","timestamp":1689988599000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jcb\/article\/112\/5\/1017\/14021\/Structure-of-the-chicken-neuron-glia-cell-adhesion"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,3,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1991,3,1]]}},"URL":"https:\/\/doi.org\/10.1083\/jcb.112.5.1017","relation":{},"ISSN":["0021-9525","1540-8140"],"issn-type":[{"value":"0021-9525","type":"print"},{"value":"1540-8140","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,3,1]]}}}