{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T05:46:33Z","timestamp":1791179193478,"version":"4.1.0"},"reference-count":0,"publisher":"Rockefeller University Press","issue":"6","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1991,3,15]]},"abstract":"<jats:p>Chemical cross-linking and gel permeation chromatography were used to examine early events in the biogenesis of class I histocompatibility molecules. We show that newly synthesized class I heavy chains associate rapidly and quantitatively with an 88-kD protein in three murine tumor cell lines. This protein (p88) does not appear to possess Asn-linked glycans and it is not the abundant ER protein, GRP94. The class I-p88 complex exists transiently (t1\/2 = 20-45 min depending on the specific class I heavy chain) and several lines of evidence suggest that p88 dissociates from the complex while still in the ER. Dissociation is not triggered upon binding of beta 2-microglobulin to the heavy chain (t1\/2 = 2-5 min). However, the rate of dissociation does correlate with the characteristic rate of ER to Golgi transport for the particular class I molecule studied. Consequently, dissociation of p88 may be rate limiting for ER to Golgi transport. Class I molecules bind antigenic peptides, apparently in the ER, for subsequent presentation to cytotoxic T lymphocytes at the cell surface. p88 could promote peptide binding or it may retain class I molecules in the ER during formation of the ternary complex of heavy chain, beta 2-microglobulin, and peptide.<\/jats:p>","DOI":"10.1083\/jcb.112.6.1099","type":"journal-article","created":{"date-parts":[[2004,5,15]],"date-time":"2004-05-15T00:18:20Z","timestamp":1084580300000},"page":"1099-1115","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":229,"title":["Participation of a novel 88-kD protein in the biogenesis of murine class I histocompatibility molecules."],"prefix":"10.1083","volume":"112","author":[{"given":"E","family":"Degen","sequence":"first","affiliation":[{"name":"Department of Biochemistry, University of Toronto, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D B","family":"Williams","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Toronto, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1991,3,15]]},"container-title":["The Journal of cell biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/112\/6\/1099\/1464345\/1099.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/112\/6\/1099\/1464345\/1099.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,22]],"date-time":"2023-07-22T01:18:09Z","timestamp":1689988689000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jcb\/article\/112\/6\/1099\/14042\/Participation-of-a-novel-88-kD-protein-in-the"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,3,15]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1991,3,15]]}},"URL":"https:\/\/doi.org\/10.1083\/jcb.112.6.1099","relation":{},"ISSN":["0021-9525","1540-8140"],"issn-type":[{"value":"0021-9525","type":"print"},{"value":"1540-8140","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,3,15]]}}}