{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T00:12:37Z","timestamp":1789949557716,"version":"4.0.1"},"reference-count":0,"publisher":"Rockefeller University Press","issue":"1","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1992,4,1]]},"abstract":"<jats:p>Integrins are heterodimeric cell surface proteins that mediate both cell-cell and cell-extracellular matrix interactions. We and others recently identified cDNAs encoding a novel integrin beta subunit, beta 7, in lymphocytes. We have now detected beta 7 mRNA in mouse TK-1 T lymphoma cells, which are known to express the putative Peyer's patch homing receptor alpha 4 beta P. We used an anti-peptide antiserum and a novel mAb against the beta 7 subunit to show that TK-1 cells express beta 7 as the only subunit associated with alpha 4. We conclude that beta 7 and beta P are identical. We also show that activated peripheral blood T cells express alpha 4 beta 7. We studied the function of alpha 4 beta 7\/alpha 4 beta P in TK-1 cells, which do not express very late antigen (VLA)-4 (alpha 4 beta 1). Cells adhered to intact fibronectin and to a fibronectin fragment containing the CS-1 region, but not to a fragment containing the RGD sequence. Adhesion to fibronectin was inhibited by antibodies to alpha 4, suggesting that alpha 4 beta 7 is a fibronectin receptor. We confirmed that alpha 4 beta 7 binds to the CS-1 region of fibronectin using affinity chromatography. TK-1 cell adhesion to the vascular cell adhesion molecule VCAM-1 was also inhibited by antibodies to alpha 4, implying that alpha 4 beta 7 also plays a role in the adherence of lymphocytes to endothelial cells. TK-1 cell binding to fibronectin and VCAM-1 is markedly increased by brief PMA stimulation. We also found that mAbs against alpha 4 and beta 7 induce homotypic clustering of TK-1 cells. Taken together these results suggest that alpha 4 beta 7\/alpha 4 beta P recognizes some or all of the same widely distributed ligands recognized by VLA-4 (alpha 4 beta 1) and that the role of alpha 4 beta 7\/alpha 4 beta P may not be restricted to lymphocyte homing.<\/jats:p>","DOI":"10.1083\/jcb.117.1.179","type":"journal-article","created":{"date-parts":[[2004,5,15]],"date-time":"2004-05-15T00:22:22Z","timestamp":1084580542000},"page":"179-189","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":253,"title":["Role of integrin alpha 4 beta 7\/alpha 4 beta P in lymphocyte adherence to fibronectin and VCAM-1 and in homotypic cell clustering"],"prefix":"10.1083","volume":"117","author":[{"given":"C","family":"R\u00fcegg","sequence":"first","affiliation":[{"name":"Department of Medicine, University of California, San Francisco 94143."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"AA","family":"Postigo","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of California, San Francisco 94143."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"EE","family":"Sikorski","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of California, San Francisco 94143."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"EC","family":"Butcher","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of California, San Francisco 94143."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R","family":"Pytela","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of California, San Francisco 94143."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"DJ","family":"Erle","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of California, San Francisco 94143."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1992,4,1]]},"container-title":["The Journal of cell biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/117\/1\/179\/1470581\/179.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/117\/1\/179\/1470581\/179.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,22]],"date-time":"2023-07-22T02:18:13Z","timestamp":1689992293000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jcb\/article\/117\/1\/179\/14336\/Role-of-integrin-alpha-4-beta-7-alpha-4-beta-P-in"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,4,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1992,4,1]]}},"URL":"https:\/\/doi.org\/10.1083\/jcb.117.1.179","relation":{},"ISSN":["0021-9525","1540-8140"],"issn-type":[{"value":"0021-9525","type":"print"},{"value":"1540-8140","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,4,1]]}}}