{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T15:40:56Z","timestamp":1791042056304,"version":"4.1.0"},"reference-count":0,"publisher":"Rockefeller University Press","issue":"2","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1992,7,15]]},"abstract":"<jats:p>We have examined the subcellular localization of p60c-src in mammalian fibroblasts. Analysis of indirect immunofluorescence by three-dimensional optical sectioning microscopy revealed a granular cytoplasmic staining that co-localized with the microtubule organizing center. Immunofluorescence experiments with antibodies against a number of membrane markers demonstrated a striking co-localization between p60c-src and the cation-dependent mannose-6-phosphate receptor (CI-MPR), a marker that identifies endosomes. Both p60c-src and the CI-MPR were found to cluster at the spindle poles throughout mitosis. In addition, treatment of interphase and mitotic cells with brefeldin A resulted in a clustering of p60c-src and CI-MPR at a peri-centriolar position. Biochemical fractionation of cellular membranes showed that a major proportion of p60c-src co-enriched with endocytic membranes. Treatment of membranes containing HRP to alter their apparent density also altered the density of p60c-src-containing membranes. Similar density shift experiments with total cellular membranes revealed that the majority of membrane-associated p60c-src in the cell is associated with endosomes, while very little is associated with plasma membranes. These results support a role for p60c-src in the regulation of endosomal membranes and protein trafficking.<\/jats:p>","DOI":"10.1083\/jcb.118.2.321","type":"journal-article","created":{"date-parts":[[2004,5,15]],"date-time":"2004-05-15T00:22:22Z","timestamp":1084580542000},"page":"321-333","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":196,"title":["Association of p60c-src with endosomal membranes in mammalian fibroblasts."],"prefix":"10.1083","volume":"118","author":[{"given":"K B","family":"Kaplan","sequence":"first","affiliation":[{"name":"Department of Microbiology, University of California, San Francisco 94143."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J R","family":"Swedlow","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of California, San Francisco 94143."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H E","family":"Varmus","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of California, San Francisco 94143."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D O","family":"Morgan","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of California, San Francisco 94143."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1992,7,15]]},"container-title":["The Journal of cell biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/118\/2\/321\/1472799\/321.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/118\/2\/321\/1472799\/321.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,22]],"date-time":"2023-07-22T02:41:59Z","timestamp":1689993719000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jcb\/article\/118\/2\/321\/28334\/Association-of-p60c-src-with-endosomal-membranes"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,7,15]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1992,7,15]]}},"URL":"https:\/\/doi.org\/10.1083\/jcb.118.2.321","relation":{},"ISSN":["0021-9525","1540-8140"],"issn-type":[{"value":"0021-9525","type":"print"},{"value":"1540-8140","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,7,15]]}}}