{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T07:04:09Z","timestamp":1791270249816,"version":"4.1.0"},"reference-count":0,"publisher":"Rockefeller University Press","issue":"6","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1993,12,15]]},"abstract":"<jats:p>We show that the alpha-spectrin gene is essential for larval survival and development by characterizing several alpha-spectrin mutations in Drosophila. P-element minigene rescue and sequence analysis were used to identify the alpha-spectrin gene as the l(3)dre3 complementation group of the Dras-Roughened-ecdysoneless region of chromosome 3 (Sliter et al., 1988). Germ line transformants carrying an alpha-spectrin cDNA, whose expression is driven by the ubiquitin promoter, fully rescued the first to second instar lethality characteristic of the l(3)dre3 alleles. The molecular defects in two gamma-ray-induced alleles were identified. One of these mutations, which resulted in second instar lethality, contained a 73-bp deletion in alpha-spectrin segment 22 (starting at amino acid residue 2312), producing a premature stop codon between the two EF hands found in this segment. The second mutation, which resulted in first instar lethality, contained a 20 base pair deletion in the middle of segment 1 (at amino acid residue 92), resulting in a premature stop codon. Examination of the spectrin-deficient larvae revealed a loss of contact between epithelial cells of the gut and disruption of cell-substratum interactions. The most pronounced morphological change was seen in tissues of complex cellular architecture such as the middle midgut where a loss of cell contact between cup-shaped cuprophilic cells and neighboring interstitial cells was accompanied by disorganization of the cuprophilic cell brush borders. Our examination of spectrin deficient larvae suggests that an important role of non-erythroid spectrin is to stabilize cell to cell interactions that are critical for the maintenance of cell shape and subcellular organization within tissues.<\/jats:p>","DOI":"10.1083\/jcb.123.6.1797","type":"journal-article","created":{"date-parts":[[2004,5,15]],"date-time":"2004-05-15T00:22:22Z","timestamp":1084580542000},"page":"1797-1809","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":123,"title":["Cell shape and interaction defects in alpha-spectrin mutants of Drosophila melanogaster."],"prefix":"10.1083","volume":"123","author":[{"given":"J K","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Cellular and Developmental Biology, Harvard University, Cambridge, Massachusetts 02138."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R S","family":"Coyne","sequence":"additional","affiliation":[{"name":"Department of Cellular and Developmental Biology, Harvard University, Cambridge, Massachusetts 02138."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R R","family":"Dubreuil","sequence":"additional","affiliation":[{"name":"Department of Cellular and Developmental Biology, Harvard University, Cambridge, Massachusetts 02138."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L S","family":"Goldstein","sequence":"additional","affiliation":[{"name":"Department of Cellular and Developmental Biology, Harvard University, Cambridge, Massachusetts 02138."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D","family":"Branton","sequence":"additional","affiliation":[{"name":"Department of Cellular and Developmental Biology, Harvard University, Cambridge, Massachusetts 02138."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1993,12,15]]},"container-title":["The Journal of cell biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/123\/6\/1797\/1479674\/1797.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/123\/6\/1797\/1479674\/1797.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,22]],"date-time":"2023-07-22T06:13:55Z","timestamp":1690006435000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jcb\/article\/123\/6\/1797\/29379\/Cell-shape-and-interaction-defects-in-alpha"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,12,15]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1993,12,15]]}},"URL":"https:\/\/doi.org\/10.1083\/jcb.123.6.1797","relation":{},"ISSN":["0021-9525","1540-8140"],"issn-type":[{"value":"0021-9525","type":"print"},{"value":"1540-8140","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,12,15]]}}}