{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T23:27:59Z","timestamp":1791588479643,"version":"4.3.4"},"reference-count":0,"publisher":"Rockefeller University Press","issue":"2","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1995,4,15]]},"abstract":"<jats:p>To examine the function of the alpha 6 beta 4 integrin we have determined its ligand-binding ability and overexpressed two potentially dominant negative mutant beta 4 subunits, lacking either the cytoplasmic or extracellular domain, in bladder epithelial 804G cells. The results of cell adhesion and radioligand-binding assays showed that alpha 6 beta 4 is a receptor for several laminin isoforms, including laminin 1, 2, 4, and 5. Overexpression of the tail-less or head-less mutant beta 4 subunit did not suppress alpha 6 beta 4-mediated adhesion to laminins, as both types of transfectants adhered to these ligands in the presence of blocking anti-beta 1 antibodies as well as the controls. However, immunofluorescence experiments indicated that the endogenous alpha 6 beta 4 integrin and other hemidesmosomal markers were not concentrated in hemidesmosomes in cells overexpressing tail-less beta 4, while the distribution of these molecules was not altered in cells overexpressing the head-less subunit. Electron microscopic studies confirmed that cells overexpressing tail-less beta 4 had a drastically reduced number of hemidesmosomes, while cells expressing the head-less subunit had a normal number of these structures. Thus, expression of a tail-less, but not a head-less mutant beta 4 subunit leads to a dominant negative effect on hemidesmosome assembly without suppressing initial adhesion to laminins. We conclude that the alpha 6 beta 4 integrin binds to several laminins and plays an essential role in the assembly and\/or stability of hemidesmosomes, that alpha 6 beta 4-mediated adhesion and hemidesmosome assembly have distinct requirements, and that it is possible to use a dominant negative approach to selectively interfere with a specific function of an integrin.<\/jats:p>","DOI":"10.1083\/jcb.129.2.473","type":"journal-article","created":{"date-parts":[[2004,5,15]],"date-time":"2004-05-15T01:23:47Z","timestamp":1084584227000},"page":"473-487","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":136,"title":["A recombinant tail-less integrin beta 4 subunit disrupts hemidesmosomes, but does not suppress alpha 6 beta 4-mediated cell adhesion to laminins."],"prefix":"10.1083","volume":"129","author":[{"given":"L","family":"Spinardi","sequence":"first","affiliation":[{"name":"Department of Pathology, New York University School of Medicine, New York 10016, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Einheber","sequence":"additional","affiliation":[{"name":"Department of Pathology, New York University School of Medicine, New York 10016, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Cullen","sequence":"additional","affiliation":[{"name":"Department of Pathology, New York University School of Medicine, New York 10016, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T A","family":"Milner","sequence":"additional","affiliation":[{"name":"Department of Pathology, New York University School of Medicine, New York 10016, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F G","family":"Giancotti","sequence":"additional","affiliation":[{"name":"Department of Pathology, New York University School of Medicine, New York 10016, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1995,4,15]]},"container-title":["The Journal of cell biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/129\/2\/473\/1478635\/473.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/129\/2\/473\/1478635\/473.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,22]],"date-time":"2023-07-22T05:54:47Z","timestamp":1690005287000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jcb\/article\/129\/2\/473\/29181\/A-recombinant-tail-less-integrin-beta-4-subunit"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,4,15]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1995,4,15]]}},"URL":"https:\/\/doi.org\/10.1083\/jcb.129.2.473","relation":{},"ISSN":["0021-9525","1540-8140"],"issn-type":[{"value":"0021-9525","type":"print"},{"value":"1540-8140","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,4,15]]}}}