{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T10:01:33Z","timestamp":1776938493675,"version":"3.51.4"},"reference-count":0,"publisher":"Rockefeller University Press","issue":"3","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1995,11,1]]},"abstract":"<jats:p>CD59, an 18-20-kD complement inhibitor anchored to the membrane via glycosyl phosphatidylinositol (GPI), can induce activation of T cells and neutrophils upon cross-linking with antibody. GPI-anchored molecules cocluster in high mol wt detergent-resistant complexes containing tyrosine kinases that are implicated in the signaling pathway. Exogenous, incorporated GPI-anchored molecules are initially unable to induce activation, presumably because they are not associated with kinases. Here we demonstrate that erythrocyte-derived CD59 incorporated in a CD59-negative cell line acquires signaling capacity in a time-dependent manner. Confocal microscopy revealed an initial diffuse distribution of CD59 that became clustered within 2 h to give a pattern similar to endogenous GPI-anchored molecules. Gel filtration of detergent-solubilized cells immediately after incorporation revealed that CD59 was mainly monomeric, but after 3 h incubation all was in high mol wt complexes and had become associated with protein kinases. Newly incorporated CD59 did not deliver a Ca2+ signal upon cross-linking, but at a time when it had become clustered and associated with kinase activity, cross-linking induced a large calcium transient, indicating that CD59 had incorporated in a specialized microenvironment that allowed it to function fully as a signal-transducing molecule.<\/jats:p>","DOI":"10.1083\/jcb.131.3.669","type":"journal-article","created":{"date-parts":[[2004,5,15]],"date-time":"2004-05-15T01:23:47Z","timestamp":1084584227000},"page":"669-677","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":107,"title":["Exogenous glycosyl phosphatidylinositol-anchored CD59 associates with kinases in membrane clusters on U937 cells and becomes Ca(2+)-signaling competent."],"prefix":"10.1083","volume":"131","author":[{"given":"C W","family":"van den Berg","sequence":"first","affiliation":[{"name":"Department of Medical Biochemistry, University of Wales College of Medicine, Cardiff, United Kingdom."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T","family":"Cinek","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry, University of Wales College of Medicine, Cardiff, United Kingdom."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M B","family":"Hallett","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry, University of Wales College of Medicine, Cardiff, United Kingdom."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V","family":"Horejsi","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry, University of Wales College of Medicine, Cardiff, United Kingdom."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B P","family":"Morgan","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry, University of Wales College of Medicine, Cardiff, United Kingdom."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[1995,11,1]]},"container-title":["The Journal of cell biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/131\/3\/669\/1479438\/669.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/131\/3\/669\/1479438\/669.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,22]],"date-time":"2023-07-22T06:08:27Z","timestamp":1690006107000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jcb\/article\/131\/3\/669\/15148\/Exogenous-glycosyl-phosphatidylinositol-anchored"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,11,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1995,11,1]]}},"URL":"https:\/\/doi.org\/10.1083\/jcb.131.3.669","relation":{},"ISSN":["0021-9525","1540-8140"],"issn-type":[{"value":"0021-9525","type":"print"},{"value":"1540-8140","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,11,1]]}}}