{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T11:46:54Z","timestamp":1773402414552,"version":"3.50.1"},"reference-count":37,"publisher":"Rockefeller University Press","issue":"2","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1997,1,27]]},"abstract":"<jats:p>This report provides evidence that the proregion of the NGF precursor protein contains two novel bioactive peptides. The presence of pairs of basic amino acid (aa) residues in the NGF proregion suggests that two or three peptides other than NGF may be generated by proteolytic cleavage. Synthetic peptides of 29 aa (LIP1) and 38aa (LIP2) corresponding to the sequences \u221271 to \u221243 and \u221240 to \u22123 of the proNGF, respectively, were used in this study. ELISA specific for these two peptides revealed their presence in the rat intestine. LIP1 was localized by immunohistochemistry in endocrine cells of the intestinal epithelium, and LIP2 was immunoprecipitated from an intestinal extract. We also provide evidence for the presence of specific receptors for LIP2 in several cell lines. Scatchard analysis indicated the presence of a low affinity binding site with a Kd of \u223c10\u22127 M and a high affinity binding site of 10\u22129 M. Cross-linking studies revealed receptor forms of about 140 kD and 93 kD in a prostatic adenocarcinoma cell line.<\/jats:p>\n               <jats:p>LIP1 and LIP2 induced rapid F-actin redistribution in PC12 cells within 2 min of incubation, which suggests a role of LIP1 and LIP2 in the process of neurite outgrowth. Furthermore, both propeptides induced rapid tyrosine phosphorylation of the Trk protein in both prostatic adenocarcinoma cells and PC12 cells, thus implicating trk in their mechanism of action. These results support our hypothesis that two peptides within the NGF precursor protein are biologically active.<\/jats:p>","DOI":"10.1083\/jcb.136.2.389","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T16:47:50Z","timestamp":1027702070000},"page":"389-398","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":32,"title":["Two Peptides Derived from the Nerve Growth Factor Precursor Are Biologically Active"],"prefix":"10.1083","volume":"136","author":[{"given":"Eleni","family":"Dicou","sequence":"first","affiliation":[{"name":"*Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale U298, Centre Hospitalier Universitaire, Angers 49033, France; \u2021Department of Cell Biology, Georgetown University Medical Center, Washington DC, 20007; \u00a7Sanofi Recherche, 31676 Lab\u00e8ge, France; and \u2016Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale U10, H\u00f4pital Bichat, 75877 Paris, Cedex 18, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beth","family":"Pflug","sequence":"additional","affiliation":[{"name":"*Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale U298, Centre Hospitalier Universitaire, Angers 49033, France; \u2021Department of Cell Biology, Georgetown University Medical Center, Washington DC, 20007; \u00a7Sanofi Recherche, 31676 Lab\u00e8ge, France; and \u2016Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale U10, H\u00f4pital Bichat, 75877 Paris, Cedex 18, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marilyn","family":"Magazin","sequence":"additional","affiliation":[{"name":"*Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale U298, Centre Hospitalier Universitaire, Angers 49033, France; \u2021Department of Cell Biology, Georgetown University Medical Center, Washington DC, 20007; \u00a7Sanofi Recherche, 31676 Lab\u00e8ge, France; and \u2016Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale U10, H\u00f4pital Bichat, 75877 Paris, Cedex 18, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Th\u00e9r\u00e8se","family":"Lehy","sequence":"additional","affiliation":[{"name":"*Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale U298, Centre Hospitalier Universitaire, Angers 49033, France; \u2021Department of Cell Biology, Georgetown University Medical Center, Washington DC, 20007; \u00a7Sanofi Recherche, 31676 Lab\u00e8ge, France; and \u2016Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale U10, H\u00f4pital Bichat, 75877 Paris, Cedex 18, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Djakiew","sequence":"additional","affiliation":[{"name":"*Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale U298, Centre Hospitalier Universitaire, Angers 49033, France; \u2021Department of Cell Biology, Georgetown University Medical Center, Washington DC, 20007; \u00a7Sanofi Recherche, 31676 Lab\u00e8ge, France; and \u2016Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale U10, H\u00f4pital Bichat, 75877 Paris, Cedex 18, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pascual","family":"Ferrara","sequence":"additional","affiliation":[{"name":"*Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale U298, Centre Hospitalier Universitaire, Angers 49033, France; \u2021Department of Cell Biology, Georgetown University Medical Center, Washington DC, 20007; \u00a7Sanofi Recherche, 31676 Lab\u00e8ge, France; and \u2016Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale U10, H\u00f4pital Bichat, 75877 Paris, Cedex 18, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V\u00e9ronique","family":"Nerri\u00e8re","sequence":"additional","affiliation":[{"name":"*Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale U298, Centre Hospitalier Universitaire, Angers 49033, France; \u2021Department of Cell Biology, Georgetown University Medical Center, Washington DC, 20007; \u00a7Sanofi Recherche, 31676 Lab\u00e8ge, France; and \u2016Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale U10, H\u00f4pital Bichat, 75877 Paris, Cedex 18, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Douglas","family":"Harvie","sequence":"additional","affiliation":[{"name":"*Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale U298, Centre Hospitalier Universitaire, Angers 49033, France; \u2021Department of Cell Biology, Georgetown University Medical Center, Washington DC, 20007; \u00a7Sanofi Recherche, 31676 Lab\u00e8ge, France; and \u2016Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale U10, H\u00f4pital Bichat, 75877 Paris, Cedex 18, 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