{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T06:19:58Z","timestamp":1791181198276,"version":"4.1.0"},"reference-count":58,"publisher":"Rockefeller University Press","issue":"1","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1997,10,6]]},"abstract":"<jats:p>In the mammalian host, the cell surface of Trypanosoma brucei is protected by a variant surface glycoprotein that is anchored in the plasma membrane through covalent attachment of the COOH terminus to a glycosylphosphatidylinositol. The trypanosome also contains a phospholipase C (GPI-PLC) that cleaves this anchor and could thus potentially enable the trypanosome to shed the surface coat of VSG. Indeed, release of the surface VSG can be observed within a few minutes on lysis of trypanosomes in vitro. To investigate whether the ability to cleave the membrane anchor of the VSG is an essential function of the enzyme in vivo, a GPI-PLC null mutant trypanosome has been generated by targeted gene deletion. The mutant trypanosomes are fully viable; they can go through an entire life cycle and maintain a persistent infection in mice. Thus the GPI-PLC is not an essential activity and is not necessary for antigenic variation. However, mice infected with the mutant trypanosomes have a reduced parasitemia and survive longer than those infected with control trypanosomes. This phenotype is partially alleviated when the null mutant is modified to express low levels of GPI-PLC.<\/jats:p>","DOI":"10.1083\/jcb.139.1.103","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T16:47:50Z","timestamp":1027702070000},"page":"103-114","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":78,"title":["The GPI-Phospholipase C of <i>Trypanosoma brucei<\/i> Is Nonessential But Influences Parasitemia in Mice"],"prefix":"10.1083","volume":"139","author":[{"given":"Helena","family":"Webb","sequence":"first","affiliation":[{"name":"*Department of Biochemistry, Cambridge University, Cambridge CB2 1QW, United Kingdom; \u2021Laboratory of Molecular Parasitology, University of Brussels, 1460 Rhode St Genese, Belgium; \u00a7Laboratory of Entomology, Institute for Tropical Medicine, B2000 Antwerpen, Belgium; and \u2016Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G11 6NU, Scotland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicola","family":"Carnall","sequence":"additional","affiliation":[{"name":"*Department of Biochemistry, Cambridge University, Cambridge CB2 1QW, United Kingdom; \u2021Laboratory of Molecular Parasitology, University of Brussels, 1460 Rhode St Genese, Belgium; \u00a7Laboratory of Entomology, Institute for Tropical Medicine, B2000 Antwerpen, Belgium; and \u2016Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G11 6NU, Scotland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luc","family":"Vanhamme","sequence":"additional","affiliation":[{"name":"*Department of Biochemistry, Cambridge University, Cambridge CB2 1QW, United Kingdom; \u2021Laboratory of Molecular Parasitology, University of Brussels, 1460 Rhode St Genese, Belgium; \u00a7Laboratory of Entomology, Institute for Tropical Medicine, B2000 Antwerpen, Belgium; and \u2016Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G11 6NU, Scotland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvie","family":"Rolin","sequence":"additional","affiliation":[{"name":"*Department of Biochemistry, Cambridge University, Cambridge CB2 1QW, United Kingdom; \u2021Laboratory of Molecular Parasitology, University of Brussels, 1460 Rhode St Genese, Belgium; \u00a7Laboratory of Entomology, Institute for Tropical Medicine, B2000 Antwerpen, Belgium; and \u2016Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G11 6NU, Scotland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jakke Van Den","family":"Abbeele","sequence":"additional","affiliation":[{"name":"*Department of Biochemistry, Cambridge University, Cambridge CB2 1QW, United Kingdom; \u2021Laboratory of Molecular Parasitology, University of Brussels, 1460 Rhode St Genese, Belgium; \u00a7Laboratory of Entomology, Institute for Tropical Medicine, B2000 Antwerpen, Belgium; and \u2016Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G11 6NU, Scotland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sue","family":"Welburn","sequence":"additional","affiliation":[{"name":"*Department of Biochemistry, Cambridge University, Cambridge CB2 1QW, United Kingdom; \u2021Laboratory of Molecular Parasitology, University of Brussels, 1460 Rhode St Genese, Belgium; \u00a7Laboratory of Entomology, Institute for Tropical Medicine, B2000 Antwerpen, Belgium; and \u2016Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G11 6NU, Scotland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Etienne","family":"Pays","sequence":"additional","affiliation":[{"name":"*Department of Biochemistry, Cambridge University, Cambridge CB2 1QW, United Kingdom; \u2021Laboratory of Molecular Parasitology, University of Brussels, 1460 Rhode St Genese, Belgium; \u00a7Laboratory of Entomology, Institute for Tropical Medicine, B2000 Antwerpen, Belgium; and \u2016Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G11 6NU, Scotland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Carrington","sequence":"additional","affiliation":[{"name":"*Department of Biochemistry, Cambridge University, Cambridge CB2 1QW, United Kingdom; \u2021Laboratory of Molecular Parasitology, University of Brussels, 1460 Rhode St Genese, Belgium; \u00a7Laboratory of Entomology, Institute for Tropical Medicine, B2000 Antwerpen, Belgium; and \u2016Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G11 6NU, Scotland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1997,10,6]]},"reference":[{"key":"2023072207094192900_B1","doi-asserted-by":"crossref","first-page":"9625","DOI":"10.1073\/pnas.86.23.9626","article-title":"Ordered appearance of antigenic variants of African trypanosomes explained in a mathematical model based on stochastic switch process and immune-selection against putative switch intermediates","volume":"86","author":"Agur","year":"1989","journal-title":"Proc Natl Acad Sci USA"},{"key":"2023072207094192900_B2","first-page":"119","article-title":"Macrophages as mediators of immunosuppression in murine African trypanosomiasis","volume":"117","author":"Askonas","year":"1985","journal-title":"Curr Top Microbiol Immunol"},{"key":"2023072207094192900_B3","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/0169-4758(91)90143-C","article-title":"The dynamics of antigenic variation and growth of African trypanosomes","volume":"7","author":"Barry","year":"1991","journal-title":"Parasitol Today"},{"key":"2023072207094192900_B4","doi-asserted-by":"crossref","first-page":"2925","DOI":"10.1002\/j.1460-2075.1995.tb07292.x","article-title":"The 3\u2032-terminal region of the mRNAs for VSG and procyclin expression in Trypanosoma brucei.","volume":"14","author":"Berberof","year":"1995","journal-title":"EMBO (Eur Mol Biol Organ) J"},{"key":"2023072207094192900_B5","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1038\/362603a0","article-title":"A structural motif in the variant surface glycoproteins of Trypanosoma brucei.","volume":"362","author":"Blum","year":"1993","journal-title":"Nature (Lond)"},{"key":"2023072207094192900_B6","first-page":"289","article-title":"Cultivation and in vitro cloning of procyclic culture forms of T. bruceiin a semi-defined medium","volume":"36","author":"Brun","year":"1979","journal-title":"Acta Trop"},{"key":"2023072207094192900_B7","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/0014-5793(85)81223-0","article-title":"Synthesis of a hydrolase for the membrane form variant surface glycoprotein is repressed during transformation of Trypanosoma brucei.","volume":"187","author":"B\u00fclow","year":"1985","journal-title":"FEBS (Fed Eur Biochem Soc) Lett"},{"key":"2023072207094192900_B8","doi-asserted-by":"crossref","first-page":"11918","DOI":"10.1016\/S0021-9258(18)67328-4","article-title":"Purification and characterization of the membrane-form variant surface glycoprotein hydrolase of Trypanosoma brucei.","volume":"261","author":"B\u00fclow","year":"1986","journal-title":"J Biol Chem"},{"key":"2023072207094192900_B9","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1242\/jcs.93.2.233","article-title":"Intracellular location of the glycosylphosphatidylinositol-specific phospholipase C of Trypanosoma brucei.","volume":"93","author":"B\u00fclow","year":"1989","journal-title":"J Cell Sci"},{"key":"2023072207094192900_B10","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/0166-6851(89)90132-1","article-title":"The release of the variable surface glycoprotein on the transformation of Trypanosoma bruceifrom bloodstream to procyclic form","volume":"32","author":"B\u00fclow","year":"1989","journal-title":"Mol Biochem Parasitol"},{"key":"2023072207094192900_B11","doi-asserted-by":"crossref","first-page":"15533","DOI":"10.1074\/jbc.271.26.15533","article-title":"Phosphatidylinositol hydrolysis by Trypanosoma bruceiglycosylphosphatidylinositol phospholipase C","volume":"271","author":"B\u00fctikofer","year":"1996","journal-title":"J Biol Chem"},{"key":"2023072207094192900_B12","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1038\/302349a0","article-title":"The membrane form of variant surface glycoproteins of Trypanosoma brucei.","volume":"302","author":"Cardoso de Almeida","year":"1983","journal-title":"Nature (Lond)"},{"key":"2023072207094192900_B13","doi-asserted-by":"crossref","first-page":"10179","DOI":"10.1093\/nar\/15.24.10179","article-title":"The structure and transcription of an element interspersed between tandem arrays of mini-exon donor RNA genes in Trypanosoma brucei.","volume":"15","author":"Carrington","year":"1987","journal-title":"Nucleic Acids Res"},{"key":"2023072207094192900_B14","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1016\/0166-6851(89)90091-1","article-title":"Sequence and expression of the glycosylphosphatidylinositol-specific phospholipase C of Trypanosoma brucei.","volume":"33","author":"Carrington","year":"1989","journal-title":"Mol Biochem Parasitol"},{"key":"2023072207094192900_B15","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1016\/0309-1651(91)90058-Q","article-title":"The biology of the glycosylphosphatidylinositol-specific phospholipase C of Trypanosoma brucei.","volume":"15","author":"Carrington","year":"1991","journal-title":"Cell Biol Int Rep"},{"key":"2023072207094192900_B16","first-page":"647","article-title":"Lipid composition of bloodstream forms of Trypanosoma brucei brucei.","volume":"83B","author":"Carroll","year":"1986","journal-title":"Comp Biochem Physiol"},{"key":"2023072207094192900_B17","first-page":"3036","article-title":"Transcription of the procyclic acidic repetitive protein genes of Trypanosoma brucei.","volume":"10","author":"Clayton","year":"1990","journal-title":"Mol Cell Biol"},{"key":"2023072207094192900_B18","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1038\/348171a0","article-title":"Gene replacement in parasitic protozoa","volume":"348","author":"Cruz","year":"1990","journal-title":"Nature (Lond)"},{"key":"2023072207094192900_B19","doi-asserted-by":"crossref","first-page":"2024","DOI":"10.1101\/gad.5.11.2024","article-title":"Homologous recombination in the tandem calmodulin genes of Trypanosoma bruceiyields multiple products: compensation for deleterious deletions by gene amplification","volume":"5","author":"Eid","year":"1991","journal-title":"Genes Dev"},{"key":"2023072207094192900_B20","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/0166-6851(92)90091-W","article-title":"Purification and characterisation of a novel sialidase found in procyclic culture forms of Trypanosoma brucei.","volume":"54","author":"Engstler","year":"1992","journal-title":"Mol Biochem Parasitol"},{"key":"2023072207094192900_B21","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1126\/science.3892689","article-title":"Expression of two variant surface glycoprotein genes on two individual African trypanosomes during antigen switching","volume":"229","author":"Esser","year":"1985","journal-title":"Science (Wash DC)"},{"key":"2023072207094192900_B22","doi-asserted-by":"crossref","first-page":"14547","DOI":"10.1016\/S0021-9258(17)38603-9","article-title":"Glycosyl-sn-1,2-dimyristylphosphatidylinositol is covalently linked to Trypanosoma bruceivariant surface glycoprotein","volume":"260","author":"Ferguson","year":"1985","journal-title":"J Biol Chem"},{"key":"2023072207094192900_B23","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1016\/S0021-9258(17)42479-3","article-title":"Biosynthesis of Trypanosoma bruceivariant glycoprotein","volume":"261","author":"Ferguson","year":"1986","journal-title":"J Biol Chem"},{"key":"2023072207094192900_B24","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1126\/science.3340856","article-title":"Glycosylphosphatidylinositol moiety that anchors Trypanosoma bruceivariant surface glycoprotein to the membrane","volume":"239","author":"Ferguson","year":"1988","journal-title":"Science (Wash DC)"},{"key":"2023072207094192900_B25","doi-asserted-by":"crossref","first-page":"2731","DOI":"10.1002\/j.1460-2075.1991.tb07821.x","article-title":"A glycosylphosphatidylinositol protein anchor from procyclic stage Trypanosoma brucei: lipid structure and biosynthesis","volume":"10","author":"Field","year":"1991","journal-title":"EMBO (Eur Mol Biol Organ) J"},{"key":"2023072207094192900_B26","doi-asserted-by":"crossref","first-page":"15767","DOI":"10.1016\/S0021-9258(18)66784-5","article-title":"Purification and characterization of a novel glycanphosphatidylinositol specific phospholipase C from Trypanosoma brucei.","volume":"261","author":"Fox","year":"1986","journal-title":"J Biol Chem"},{"key":"2023072207094192900_B27","doi-asserted-by":"crossref","first-page":"3080","DOI":"10.1002\/j.1460-2075.1995.tb07311.x","article-title":"The role of inositol acylation and inosotol deacylation in GPI biosynthesis in Trypanosoma brucei.","volume":"14","author":"G\u00fcther","year":"1995","journal-title":"EMBO (Eur Mol Biol Organ) J"},{"key":"2023072207094192900_B28","first-page":"121","article-title":"The role of glycolipid C in the glycosylphosphatidylinositol biosynthetic pathway in Trypanosoma bruceibloodstream forms","volume":"27","author":"G\u00fcther","year":"1994","journal-title":"Braz J Med Biol Res"},{"key":"2023072207094192900_B29","doi-asserted-by":"crossref","first-page":"13813","DOI":"10.1016\/S0021-9258(18)67092-9","article-title":"A phospholipase C from Trypanosoma bruceiwhich selectively cleaves the glycolipid on the variant surface glycoprotein","volume":"261","author":"Hereld","year":"1986","journal-title":"J Biol Chem"},{"key":"2023072207094192900_B30","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/0166-6851(81)90095-5","article-title":"Glycopeptides from variant surface glycoproteins of Trypanosoma brucei.C-terminal location of antigenically cross-reacting carbohydrate moitities","volume":"2","author":"Holder","year":"1981","journal-title":"Mol Biochem Parasitol"},{"key":"2023072207094192900_B31","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1042\/bj2300195","article-title":"A new method for the rapid purification of both the membrane-bound and released forms of the variant surface glycoprotein from Trypanosoma brucei.","volume":"230","author":"Jackson","year":"1985","journal-title":"Biochem J"},{"key":"2023072207094192900_B32","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1042\/bj1780689","article-title":"Selective cleavage of variant surface glycoproteins from Trypanosoma brucei.","volume":"178","author":"Johnson","year":"1979","journal-title":"Biochem J"},{"key":"2023072207094192900_B33","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1016\/0014-4894(70)90120-7","article-title":"Isolation of salivarian trypanosomes from man and other mammals using DEAE-cellulose","volume":"28","author":"Lanham","year":"1970","journal-title":"Exp Parsitol"},{"key":"2023072207094192900_B34","doi-asserted-by":"crossref","first-page":"1583","DOI":"10.1126\/science.2177225","article-title":"Homologous recombination and stable transfection in the parasitic protozoan Trypanosoma brucei.","volume":"250","author":"Lee","year":"1990","journal-title":"Science (Wash DC)"},{"key":"2023072207094192900_B35","doi-asserted-by":"crossref","first-page":"2041","DOI":"10.1002\/j.1460-2075.1991.tb07734.x","article-title":"Phenylmethanesulphonyl fluoride inhibits GPI anchor biosynthesis in the African trypanosome","volume":"10","author":"Masterson","year":"1991","journal-title":"EMBO (Eur Mol Biol Organ) J"},{"key":"2023072207094192900_B36","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1042\/bj2940305","article-title":"The structure, biosynthesis and function of glycosylated phosphatidyinositols in the parasitic protozoa and higher eukaryotes","volume":"294","author":"McConville","year":"1993","journal-title":"Biochem J"},{"key":"2023072207094192900_B37","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1016\/0166-6851(92)90180-R","article-title":"Glycosyl phosphatidylinositol-specific phospholipase C of Trypanosoma brucei: expression in Escherichia coli.","volume":"56","author":"Mensa-Wilmot","year":"1992","journal-title":"Mol Biochem Parasitol"},{"key":"2023072207094192900_B38","first-page":"720","article-title":"Genomic organisation, chromosomal localisation, and developmentally regulated expression of the glycosylphosphatidylinositol-specific phospholipase C of Trypanosoma brucei.","volume":"10","author":"Mensa-Wilmot","year":"1990","journal-title":"Mol Cell Biol"},{"key":"2023072207094192900_B39","doi-asserted-by":"crossref","first-page":"935","DOI":"10.1083\/jcb.124.6.935","article-title":"A glycosylphosphatidylinositol (GPI)-negative phenotype produced in Leishmania major by GPI phospholipase C from Trypanosoma brucei: topography of two GPI pathways","volume":"124","author":"Mensa-Wilmot","year":"1994","journal-title":"J Cell Biol"},{"key":"2023072207094192900_B40","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1017\/S0031182000041871","article-title":"Analysis of antigenic types appearing in first relapse populations of clones of Trypanosoma brucei.","volume":"82","author":"Miller","year":"1981","journal-title":"Parasitology"},{"key":"2023072207094192900_B41","first-page":"223","article-title":"The effect of citrate\/cis-aconitate on oxidative metabolism during transformation of Trypanosoma brucei.","volume":"113","author":"Overath","year":"1986","journal-title":"Eur J Biochem"},{"key":"2023072207094192900_B42","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1083\/jcb.108.2.737","article-title":"Procyclin gene expression and loss of the variant surface glycoprotein during differentiation of Trypanosoma brucei.","volume":"108","author":"Roditi","year":"1989","journal-title":"J Cell Biol"},{"key":"2023072207094192900_B43","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/0166-6851(93)90164-S","article-title":"Transient adenylate cyclase activation accompanies differentiation of Trypanosoma bruceifrom bloodstream to procyclic forms","volume":"61","author":"Rolin","year":"1993","journal-title":"Mol Biol Parasitol"},{"key":"2023072207094192900_B44","doi-asserted-by":"crossref","first-page":"10844","DOI":"10.1074\/jbc.271.18.10844","article-title":"Simultaneous but independent activation of adenylate cyclase and glycosylphosphatidylinositol-phospholipase C under stress conditions in Trypanosoma brucei.","volume":"271","author":"Rolin","year":"1996","journal-title":"J Biol Chem"},{"key":"2023072207094192900_B45","doi-asserted-by":"crossref","first-page":"546","DOI":"10.1111\/j.1550-7408.1990.tb01263.x","article-title":"Degradation, recycling, and shedding of Trypanosoma bruceivariant surface glycoprotein","volume":"37","author":"Seyfang","year":"1990","journal-title":"J Protozool"},{"key":"2023072207094192900_B46","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1038\/348174a0","article-title":"Targeted insertion of the neomycin phosphotransferase gene into the tubulin gene cluster of Trypanosoma brucei.","volume":"348","author":"ten Asbroek","year":"1990","journal-title":"Nature (Lond)"},{"key":"2023072207094192900_B47","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/0166-6851(93)90014-O","article-title":"Stable transformation of Trypanosoma brucei.","volume":"59","author":"ten Asbroek","year":"1993","journal-title":"Mol Bioch Parasitol"},{"key":"2023072207094192900_B48","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/0169-4758(90)90385-H","article-title":"The use of experimental artefacts in African trypanosome research","volume":"6","author":"Turner","year":"1990","journal-title":"Parasitol Today"},{"key":"2023072207094192900_B49","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1017\/S0031182000081841","article-title":"Replication, differentiation, growth and the virulence of Trypanosoma bruceiinfections","volume":"111","author":"Turner","year":"1995","journal-title":"Parasitology"},{"key":"2023072207094192900_B50","first-page":"1","article-title":"Antigenic variation in syringe-passaged populations of Trypanosoma (Trypanozoon) brucei.","volume":"55","author":"Van Meirvenne","year":"1975","journal-title":"Ann Soc Belge Med Trop"},{"key":"2023072207094192900_B51","doi-asserted-by":"crossref","first-page":"987","DOI":"10.1083\/jcb.121.5.987","article-title":"Early lipid intermediates in glycosyl-phosphatidylinositol anchor assembly are synthesised in the ER and located in the cytoplasmic leaflet of the ER membrane bilayer","volume":"121","author":"Vidugiriene","year":"1993","journal-title":"J Cell Biol"},{"key":"2023072207094192900_B52","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1083\/jcb.127.2.333","article-title":"The GPI anchor of cell-surface proteins is synthesized on the cytoplasmic face of the endoplasmic reticulum","volume":"127","author":"Vidugiriene","year":"1994","journal-title":"J Cell Biol"},{"key":"2023072207094192900_B53","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1111\/j.1432-1033.1980.tb06160.x","article-title":"\u201cSwell dialysis\u201d demonstrates that adenylate cyclase in Trypanosoma bruceiis regulated by calcium ions","volume":"113","author":"Voorheis","year":"1980","journal-title":"Eur J Biochem"},{"key":"2023072207094192900_B54","doi-asserted-by":"crossref","first-page":"2300","DOI":"10.1016\/S0021-9258(18)34921-4","article-title":"Characteristics of the release of the surface coat protein from bloodstream forms of Trypanosoma brucei.","volume":"257","author":"Voorheis","year":"1982","journal-title":"J Biol Chem"},{"key":"2023072207094192900_B55","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1111\/j.1432-1033.1988.tb14310.x","article-title":"Characterisation of the cross-reacting determinant (CRD) of the glycosyl-phosphatidylinositol membrane anchor of Trypanosoma bruceivariant surface glycoprotein","volume":"176","author":"Zamze","year":"1988","journal-title":"Eur J Biochem"},{"key":"2023072207094192900_B56","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1111\/j.1432-1033.1990.tb19237.x","article-title":"Synchronous differentiation of Trypanosoma brucei from bloodstream to procyclic forms in vitro.","volume":"192","author":"Ziegelbauer","year":"1990","journal-title":"Eur J Biochem"},{"key":"2023072207094192900_B57","doi-asserted-by":"crossref","first-page":"3737","DOI":"10.1021\/bi00065a028","article-title":"Proteolytic release of cell surface proteins during differentiation of Trypanosoma brucei.","volume":"32","author":"Ziegelbauer","year":"1993","journal-title":"Biochemistry"},{"key":"2023072207094192900_B58","first-page":"227","article-title":"Electric field mediated fusion and related electrical phenomena","volume":"694","author":"Zimmerman","year":"1982","journal-title":"Biochem Biophys Acta"}],"container-title":["The Journal of Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/139\/1\/103\/1486906\/10860.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/139\/1\/103\/1486906\/10860.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,22]],"date-time":"2023-07-22T07:10:59Z","timestamp":1690009859000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jcb\/article\/139\/1\/103\/738\/The-GPI-Phospholipase-C-of-Trypanosoma-brucei-Is"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,10,6]]},"references-count":58,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1997,10,6]]}},"URL":"https:\/\/doi.org\/10.1083\/jcb.139.1.103","relation":{},"ISSN":["0021-9525","1540-8140"],"issn-type":[{"value":"0021-9525","type":"print"},{"value":"1540-8140","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,10,6]]}}}