{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T03:27:51Z","timestamp":1791343671717,"version":"4.3.1"},"reference-count":69,"publisher":"Rockefeller University Press","issue":"7","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1997,12,29]]},"abstract":"<jats:p>PML and Sp100 proteins are associated with nuclear domains, known as nuclear dots (NDs). They were discovered in the context of leukemic transformation and as an autoantigen in primary biliary cirrhosis, respectively. Both proteins are expressed in the form of many COOH-terminally spliced variants, and their expression is enhanced by interferons (IFN). The recent finding that PIC1\/SUMO-1, a small ubiquitin-like protein, is covalently linked to the RanGAP1 protein of the nuclear pore complex and also binds PML in yeast cells led us to determine whether PML is covalently modified by PIC1\/SUMO-1 and whether the same is true for Sp100. We found an immune reaction of PML and Sp100 proteins with a PIC1\/SUMO-1\u2013specific monoclonal antibody by immunoblotting when using cell extracts prepared from stably transfected cells inducibly expressing one isoform of each protein as well as from nontransfected cells. In contrast, both proteins did not react when synthesized in vitro. Immunofluorescence staining showed that PIC1\/SUMO-1 colocalized with Sp100 and PML in NDs except in mitotic cells, in which PML and Sp100 are dissociated. Cell fractionation and immunoblotting demonstrated that PIC1\/SUMO-1 immunoreactive Sp100 in IFN-treated and untreated cells was exclusively nuclear, whereas nonmodified Sp100 was also found in the cytoplasm. Taken together, these data strongly suggest covalent modification of specific nuclear isoforms of Sp100 and PML by PIC1\/SUMO-1. This modification may play a regulatory role in ND structure, composition, and function.<\/jats:p>","DOI":"10.1083\/jcb.139.7.1621","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T16:47:50Z","timestamp":1027702070000},"page":"1621-1634","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":283,"title":["Evidence for Covalent Modification of the Nuclear Dot\u2013associated Proteins PML and Sp100 by PIC1\/SUMO-1"],"prefix":"10.1083","volume":"139","author":[{"given":"Thomas","family":"Sternsdorf","sequence":"first","affiliation":[{"name":"Heinrich-Pette-Institut f\u00fcr Experimentelle Virologie und Immunologie an der Universit\u00e4t Hamburg, D-20251 Hamburg, Federal Republic of Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirsten","family":"Jensen","sequence":"additional","affiliation":[{"name":"Heinrich-Pette-Institut f\u00fcr Experimentelle Virologie und Immunologie an der Universit\u00e4t Hamburg, D-20251 Hamburg, Federal Republic of Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans","family":"Will","sequence":"additional","affiliation":[{"name":"Heinrich-Pette-Institut f\u00fcr Experimentelle Virologie und Immunologie an der Universit\u00e4t Hamburg, D-20251 Hamburg, Federal Republic of Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1997,12,29]]},"reference":[{"key":"2023072207235421300_B1","first-page":"2307","article-title":"Growth suppression of acute promyelocytic leukemia cells having increased expression of the non-rearranged alleles: RAR\u03b1 or PML","volume":"10","author":"Ahn","year":"1995","journal-title":"Oncogene"},{"key":"2023072207235421300_B2","doi-asserted-by":"crossref","first-page":"2707","DOI":"10.1002\/j.1460-2075.1996.tb00631.x","article-title":"A retrovirus carrying the promyelocyte-retinoic acid receptor 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erythropoietin","volume":"140","author":"Kitamura","year":"1989","journal-title":"J Cell Physiol"},{"key":"2023072207235421300_B37","doi-asserted-by":"crossref","first-page":"1073","DOI":"10.1002\/j.1460-2075.1994.tb06356.x","article-title":"The t(15;17) translocation alters a nuclear body in a retinoic acid-reversible fashion","volume":"13","author":"Koken","year":"1994","journal-title":"EMBO (Eur Mol Biol Organ) J"},{"key":"2023072207235421300_B38","first-page":"1315","article-title":"The PML growth-suppressor has an altered expression in human oncogenesis","volume":"10","author":"Koken","year":"1995","journal-title":"Oncogene"},{"key":"2023072207235421300_B39","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1006\/excr.1996.0353","article-title":"The nuclear domain 10 (ND10) is disrupted by the human cytomegalovirus gene product IE1","volume":"229","author":"Korioth","year":"1996","journal-title":"Exp Cell Res"},{"key":"2023072207235421300_B40","doi-asserted-by":"crossref","first-page":"2958","DOI":"10.1073\/pnas.93.7.2958","article-title":"Mammalian ubiquitin-conjugating enzyme Ubc9 interacts with Rad51 recombination protein and localizes in synaptonemal complexes","volume":"93","author":"Kovalenko","year":"1996","journal-title":"Proc Natl Acad Sci USA"},{"key":"2023072207235421300_B41","first-page":"871","article-title":"The acute promyelocytic leukaemia-associated PML gene is induced by interferon","volume":"11","author":"Lavau","year":"1995","journal-title":"Oncogene"},{"key":"2023072207235421300_B42","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1074\/jbc.271.1.130","article-title":"Analysis of the growth and transformation suppressor domains of promyelocytic leukemia gene, PML","volume":"271","author":"Le","year":"1996","journal-title":"J Biol 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