{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T17:03:51Z","timestamp":1791133431935,"version":"4.1.0"},"reference-count":37,"publisher":"Rockefeller University Press","issue":"1","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1998,4,6]]},"abstract":"<jats:p>In polarized epithelial cells, agonists trigger Ca2+ waves and oscillations. These patterns may be caused by the compartmentalization of inositol 1,4,5-trisphosphate (IP3)-sensitive Ca2+ pools into specific regions. We have investigated the relationship between the distribution of IP3 receptors (IP3Rs) and the spatiotemporal pattern of Ca2+ signaling in the duct cells of the rat submandibular gland (SMG). Using immunofluorescence, although labeling was somewhat heterogeneous, the IP3Rs were colocalized to the apical pole of the duct cells. Immunoelectron microscopy identified small apical vesicles bearing IP3R2 in some types of duct cells. Real-time confocal imaging of intact ducts demonstrated that, after carbachol stimulation, an initial Ca2+ spike occurred in the apical region. Subsequently, repetitive Ca2+ spikes spread from the apical to the middle cytoplasm. These apical Ca2+ initiation sites were found only in some \u201cpioneer cells,\u201d rather than in all duct cells. We performed both Ca2+ imaging and immunofluorescence on the same ducts and detected the strongest immunosignals of IP3R2 in the Ca2+ initiation sites of the pioneer cells. The subcellular localization and expression level of IP3Rs correlated strongly with the spatiotemporal nature of the intracellular Ca2+ signal and distinct Ca2+ responses among the rat SMG duct cells.<\/jats:p>","DOI":"10.1083\/jcb.141.1.135","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T16:47:50Z","timestamp":1027702070000},"page":"135-142","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":50,"title":["Apical Vesicles Bearing Inositol 1,4,5-trisphosphate Receptors in the Ca2+Initiation Site of Ductal Epithelium of Submandibular Gland"],"prefix":"10.1083","volume":"141","author":[{"given":"Miki","family":"Yamamoto-Hino","sequence":"first","affiliation":[{"name":"*Developmental Neurobiology Laboratory, RIKEN Brain Science Institute, Wako-City, Saitama 351, Japan; \u2021Department of Molecular Neurobiology, The Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo 108, Japan; \u00a7Department of Anatomy and Cell Biology, Faculty of Medicine, Kitasato University, Sagamihara-shi, Kanagawa 228, Japan; \u2016Department of Anatomy, School of Medicine, Gunma University, Maebashi-shi, Gunma 371, Japan; and \u00b6Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., Machida-shi, Tokyo 194, Japan"},{"name":"*Developmental Neurobiology Laboratory, RIKEN Brain Science Institute, Wako-City, Saitama 351, Japan; \u2021Department of Molecular Neurobiology, The Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo 108, Japan; \u00a7Department of Anatomy and Cell Biology, Faculty of Medicine, Kitasato University, Sagamihara-shi, Kanagawa 228, Japan; \u2016Department of Anatomy, School of Medicine, Gunma University, Maebashi-shi, Gunma 371, Japan; and \u00b6Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., Machida-shi, Tokyo 194, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsushi","family":"Miyawaki","sequence":"additional","affiliation":[{"name":"*Developmental Neurobiology Laboratory, RIKEN Brain Science Institute, Wako-City, Saitama 351, Japan; \u2021Department of Molecular Neurobiology, The Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo 108, Japan; \u00a7Department of Anatomy and Cell Biology, Faculty of Medicine, Kitasato University, Sagamihara-shi, Kanagawa 228, Japan; \u2016Department of Anatomy, School of Medicine, Gunma University, Maebashi-shi, Gunma 371, Japan; and \u00b6Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., Machida-shi, Tokyo 194, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akihisa","family":"Segawa","sequence":"additional","affiliation":[{"name":"*Developmental Neurobiology Laboratory, RIKEN Brain Science Institute, Wako-City, Saitama 351, Japan; \u2021Department of Molecular Neurobiology, The Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo 108, Japan; \u00a7Department of Anatomy and Cell Biology, Faculty of Medicine, Kitasato University, Sagamihara-shi, Kanagawa 228, Japan; \u2016Department of Anatomy, School of Medicine, Gunma University, Maebashi-shi, Gunma 371, Japan; and \u00b6Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., Machida-shi, Tokyo 194, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eijiro","family":"Adachi","sequence":"additional","affiliation":[{"name":"*Developmental Neurobiology Laboratory, RIKEN Brain Science Institute, Wako-City, Saitama 351, Japan; \u2021Department of Molecular Neurobiology, The Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo 108, Japan; \u00a7Department of Anatomy and Cell Biology, Faculty of Medicine, Kitasato University, Sagamihara-shi, Kanagawa 228, Japan; \u2016Department of Anatomy, School of Medicine, Gunma University, Maebashi-shi, Gunma 371, Japan; and \u00b6Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., Machida-shi, Tokyo 194, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shohei","family":"Yamashina","sequence":"additional","affiliation":[{"name":"*Developmental Neurobiology Laboratory, RIKEN Brain Science Institute, Wako-City, Saitama 351, Japan; \u2021Department of Molecular Neurobiology, The Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo 108, Japan; \u00a7Department of Anatomy and Cell Biology, Faculty of Medicine, Kitasato University, Sagamihara-shi, Kanagawa 228, Japan; \u2016Department of Anatomy, School of Medicine, Gunma University, Maebashi-shi, Gunma 371, Japan; and \u00b6Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., Machida-shi, Tokyo 194, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toyoshi","family":"Fujimoto","sequence":"additional","affiliation":[{"name":"*Developmental Neurobiology Laboratory, RIKEN Brain Science Institute, Wako-City, Saitama 351, Japan; \u2021Department of Molecular Neurobiology, The Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo 108, Japan; \u00a7Department of Anatomy and Cell Biology, Faculty of Medicine, Kitasato University, Sagamihara-shi, Kanagawa 228, Japan; \u2016Department of Anatomy, School of Medicine, Gunma University, Maebashi-shi, Gunma 371, Japan; and \u00b6Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., Machida-shi, Tokyo 194, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoyasu","family":"Sugiyama","sequence":"additional","affiliation":[{"name":"*Developmental Neurobiology Laboratory, RIKEN Brain Science Institute, Wako-City, Saitama 351, Japan; \u2021Department of Molecular Neurobiology, The Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo 108, Japan; \u00a7Department of Anatomy and Cell Biology, Faculty of Medicine, Kitasato University, Sagamihara-shi, Kanagawa 228, Japan; \u2016Department of Anatomy, School of Medicine, Gunma University, Maebashi-shi, Gunma 371, Japan; and \u00b6Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., Machida-shi, Tokyo 194, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teiichi","family":"Furuichi","sequence":"additional","affiliation":[{"name":"*Developmental Neurobiology Laboratory, RIKEN Brain Science Institute, Wako-City, Saitama 351, Japan; \u2021Department of Molecular Neurobiology, The Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo 108, Japan; \u00a7Department of Anatomy and Cell Biology, Faculty of Medicine, Kitasato University, Sagamihara-shi, Kanagawa 228, Japan; \u2016Department of Anatomy, School of Medicine, Gunma University, Maebashi-shi, Gunma 371, Japan; and \u00b6Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., Machida-shi, Tokyo 194, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mamoru","family":"Hasegawa","sequence":"additional","affiliation":[{"name":"*Developmental Neurobiology Laboratory, RIKEN Brain Science Institute, Wako-City, Saitama 351, Japan; \u2021Department of Molecular Neurobiology, The Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo 108, Japan; \u00a7Department of Anatomy and Cell Biology, Faculty of Medicine, Kitasato University, Sagamihara-shi, Kanagawa 228, Japan; \u2016Department of Anatomy, School of Medicine, Gunma University, Maebashi-shi, Gunma 371, Japan; and \u00b6Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., Machida-shi, Tokyo 194, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katsuhiko","family":"Mikoshiba","sequence":"additional","affiliation":[{"name":"*Developmental Neurobiology Laboratory, RIKEN Brain Science Institute, Wako-City, Saitama 351, Japan; \u2021Department of Molecular Neurobiology, The Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo 108, Japan; \u00a7Department of Anatomy and Cell Biology, Faculty of Medicine, Kitasato University, Sagamihara-shi, Kanagawa 228, Japan; \u2016Department of Anatomy, School of Medicine, Gunma University, Maebashi-shi, Gunma 371, Japan; and \u00b6Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., Machida-shi, Tokyo 194, Japan"},{"name":"*Developmental Neurobiology Laboratory, RIKEN Brain Science Institute, Wako-City, Saitama 351, Japan; \u2021Department of Molecular Neurobiology, The Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo 108, Japan; \u00a7Department of Anatomy and Cell Biology, Faculty of Medicine, Kitasato University, Sagamihara-shi, Kanagawa 228, Japan; \u2016Department of Anatomy, School of Medicine, Gunma University, Maebashi-shi, Gunma 371, Japan; and \u00b6Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., Machida-shi, Tokyo 194, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1998,4,6]]},"reference":[{"key":"2023072207384158300_B1","doi-asserted-by":"crossref","first-page":"1812","DOI":"10.1126\/science.1465619","article-title":"Range of messenger action of calcium ion and inositol 1,4,5-trisphosphate","volume":"258","author":"Allbritton","year":"1992","journal-title":"Science"},{"key":"2023072207384158300_B2","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1038\/361315a0","article-title":"Inositol trisphosphate and calcium signaling","volume":"361","author":"Berridge","year":"1993","journal-title":"Nature"},{"key":"2023072207384158300_B3","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1113\/jphysiol.1997.sp021927","article-title":"Elementary and global aspects of calcium signalling","volume":"499","author":"Berridge","year":"1997","journal-title":"J Physiol (Lond)"},{"key":"2023072207384158300_B4","doi-asserted-by":"crossref","first-page":"c490","DOI":"10.1152\/ajpcell.1991.261.3.C490","article-title":"Isolation and characterization of rat submandibular intralobular ducts","volume":"261","author":"Dehaye","year":"1991","journal-title":"Am J Physiol"},{"key":"2023072207384158300_B5","doi-asserted-by":"crossref","first-page":"c1356","DOI":"10.1152\/ajpcell.1993.265.5.C1356","article-title":"Beta-adrenergic stimulation and cAMP mobilize Ca2+ from an IP3-insensitive pool in rat submandibular granular ducts","volume":"265","author":"Dehaye","year":"1993","journal-title":"Am J Physiol"},{"key":"2023072207384158300_B6","first-page":"11","article-title":"Widespread expression of inositol 1,4,5-trisphosphate receptor type 1 gene (Insp3r1) in the mouse central nervous system","volume":"1","author":"Furuichi","year":"1993","journal-title":"Recept Channels"},{"key":"2023072207384158300_B7","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1016\/0959-4388(94)90089-2","article-title":"Intracellular channels [published erratum appears on 4(5):758]","volume":"4","author":"Furuichi","year":"1994","journal-title":"Curr Opin Neurobiol"},{"key":"2023072207384158300_B8","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1083\/jcb.119.3.493","article-title":"Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation","volume":"119","author":"Gavrieli","year":"1992","journal-title":"J Cell Biol"},{"key":"2023072207384158300_B9","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1126\/science.273.5274.503","article-title":"Lymphocyte apoptosis: mediation by increased type 3 inositol 1,4,5-trisphosphate receptor","volume":"273","author":"Khan","year":"1996","journal-title":"Science"},{"key":"2023072207384158300_B10","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1038\/348735a0","article-title":"Cytosolic Ca2+gradients triggering unidirectional fluid secretion from exocrine pancreas","volume":"348","author":"Kasai","year":"1990","journal-title":"Nature"},{"key":"2023072207384158300_B11","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/0166-2236(94)90112-0","article-title":"Spatial dynamics of second messengers: IP3and cAMP as long-range and associative messengers","volume":"17","author":"Kasai","year":"1994","journal-title":"Trends Neurosci"},{"key":"2023072207384158300_B12","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1016\/0092-8674(93)90514-Q","article-title":"Subcellular distribution of Ca2+ release channels underlying Ca2+waves and oscillations in exocrine pancreas","volume":"74","author":"Kasai","year":"1993","journal-title":"Cell"},{"key":"2023072207384158300_B13","first-page":"327","article-title":"An improved immuno-electron microscopy with a use of tannic acid and chitosan infiltration","volume":"45","author":"Katsumata","year":"1996","journal-title":"J Electron Microsc"},{"key":"2023072207384158300_B14","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1038\/bjc.1972.33","article-title":"Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics","volume":"26","author":"Kerr","year":"1972","journal-title":"Br J Cancer"},{"key":"2023072207384158300_B15","doi-asserted-by":"crossref","first-page":"15765","DOI":"10.1074\/jbc.272.25.15765","article-title":"Polarized expression of Ca2+channels in pancreatic and salivary gland cells","volume":"272","author":"Lee","year":"1997","journal-title":"J Biol Chem"},{"key":"2023072207384158300_B16","doi-asserted-by":"crossref","first-page":"15771","DOI":"10.1074\/jbc.272.25.15771","article-title":"Polarized expression of Ca2+pumps in pancreatic and salivary gland cells","volume":"272","author":"Lee","year":"1997","journal-title":"J Biol Chem"},{"key":"2023072207384158300_B17","doi-asserted-by":"crossref","first-page":"1109","DOI":"10.1016\/S0021-9258(17)35289-4","article-title":"Structural and functional characterization of inositol 1,4,5-trisphosphate receptor channel from mouse cerebellum","volume":"266","author":"Maeda","year":"1991","journal-title":"J Biol Chem"},{"key":"2023072207384158300_B18","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1038\/342192a0","article-title":"Putative receptor for inositol 1,4,5-trisphosphate similar to ryanodine receptor","volume":"342","author":"Mignery","year":"1989","journal-title":"Nature"},{"key":"2023072207384158300_B19","doi-asserted-by":"crossref","first-page":"12679","DOI":"10.1016\/S0021-9258(19)38397-8","article-title":"Structure and expression of the rat inositol 1,4,5-trisphosphate receptor","volume":"265","author":"Mignery","year":"1990","journal-title":"J Biol Chem"},{"key":"2023072207384158300_B20","doi-asserted-by":"crossref","first-page":"14700","DOI":"10.1074\/jbc.270.24.14700","article-title":"Heterotetrameric complex formation of inositol 1,4,5-trisphosphate receptor subunits","volume":"270","author":"Monkawa","year":"1995","journal-title":"J Biol Chem"},{"key":"2023072207384158300_B21","doi-asserted-by":"crossref","first-page":"6735","DOI":"10.1016\/S0021-9258(17)37437-9","article-title":"Cyclic AMP- dependent phosphorylation of an immunoaffinity-purified homotetrameric inositol 1,4,5-trisphosphate receptor (type I) increases Ca2+flux in reconstituted lipid vesicles","volume":"269","author":"Nakade","year":"1994","journal-title":"J Biol Chem"},{"key":"2023072207384158300_B22","first-page":"g338","article-title":"Multistep mechanism of polarized Ca2+wave patterns in hepatocytes","volume":"267","author":"Nathanson","year":"1994","journal-title":"Am J Physiol"},{"key":"2023072207384158300_B23","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1152\/physrev.1994.74.3.595","article-title":"Molecular and cellular physiology of intracellular calcium stores","volume":"74","author":"Pozzan","year":"1994","journal-title":"Physiol Rev"},{"key":"2023072207384158300_B24","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1002\/ar.1092200304","article-title":"Ultrastructure of the main excretory duct epithelia of the rat parotid and submandibular glands with a review of the literature","volume":"220","author":"Sato","year":"1988","journal-title":"Anat Rec"},{"key":"2023072207384158300_B25","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1016\/0003-2697(87)90587-2","article-title":"Tricine-sodium dodecyl sulfate-PAGE for the separation of proteins in the range from 1 to 100 kD","volume":"166","author":"Schagger","year":"1987","journal-title":"Anal Biochem"},{"key":"2023072207384158300_B26","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1242\/jcs.78.1.67","article-title":"Acinar structure and membrane regionalization as a prerequisite for exocrine secretion in the rat submandibular gland","volume":"78","author":"Segawa","year":"1985","journal-title":"J Cell Sci"},{"key":"2023072207384158300_B27","first-page":"327","article-title":"Ultrastructural immunolocalization of IP3receptor in the rat submandibular gland duct cells","volume":"45","author":"Segawa","year":"1996","journal-title":"J Electron Microsc"},{"key":"2023072207384158300_B28","doi-asserted-by":"crossref","first-page":"3199","DOI":"10.1002\/j.1460-2075.1991.tb04882.x","article-title":"Structure of a novel InsP3 receptor","volume":"10","author":"Sudhof","year":"1991","journal-title":"EMBO (Eur Mol Biol Organ) J"},{"key":"2023072207384158300_B29","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/0014-5793(94)01099-4","article-title":"Monoclonal antibodies distinctively recognizing the subtypes of inositol 1,4,5-trisphosphate receptor: application to the studies on inflammatory cells","volume":"354","author":"Sugiyama","year":"1994","journal-title":"FEBS Lett"},{"key":"2023072207384158300_B30","doi-asserted-by":"crossref","first-page":"1237","DOI":"10.1177\/44.11.8918898","article-title":"Subtype-specific expression patterns of inositol 1,4,5-trisphosphate receptors in rat airway epithelial cells","volume":"44","author":"Sugiyama","year":"1996","journal-title":"J Histochem Cytochem"},{"key":"2023072207384158300_B31","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1016\/0092-8674(93)90513-P","article-title":"Local and global cytosolic Ca2+oscillations in exocrine cells evoked by agonists and inositol trisphosphate","volume":"74","author":"Thorn","year":"1993","journal-title":"Cell"},{"key":"2023072207384158300_B32","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1002\/j.1460-2075.1996.tb00436.x","article-title":"Multiple, coordinated Ca2+- release events underlie the inositol trisphosphate-induced local Ca2+spikes in mouse pancreatic acinar cells","volume":"15","author":"Thorn","year":"1996","journal-title":"EMBO (Eur Mol Biol Organ) J"},{"key":"2023072207384158300_B33","first-page":"G359","article-title":"Effects of secretagogues on cytosolic Ca2+levels in rat submandibular granular ducts and acini","volume":"261","author":"Valdez","year":"1991","journal-title":"Am J Physiol"},{"key":"2023072207384158300_B34","doi-asserted-by":"crossref","first-page":"1091","DOI":"10.1073\/pnas.85.4.1091","article-title":"\u201cCalciosome,\u201d a cytoplasmic organelle: the inositol 1,4,5-trisphosphate-sensitive Ca2+ store of nonmuscle cells?","volume":"85","author":"Volpe","year":"1988","journal-title":"Proc Natl Acad Sci USA"},{"key":"2023072207384158300_B35","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1113\/jphysiol.1996.sp021246","article-title":"Characterization, localization and axial distribution of Ca2+signaling receptors in the rat submandibular salivary gland ducts","volume":"491","author":"Xu","year":"1996","journal-title":"J Physiol (Lond)"},{"key":"2023072207384158300_B36","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1042\/bj3020781","article-title":"Human inositol 1,4,5-trisphosphate type 1 receptor, InsP3R1: structure, function, regulation of expression and chromosomal localization","volume":"302","author":"Yamada","year":"1994","journal-title":"Biochem J"},{"key":"2023072207384158300_B37","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1113\/jphysiol.1995.sp020538","article-title":"Quantal puffs of intracellular Ca2+evoked by inositol trisphosphate in Xenopus oocytes","volume":"482","author":"Yao","year":"1995","journal-title":"J Physiol (Lond)"}],"container-title":["The Journal of Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/141\/1\/135\/1491076\/32953.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/141\/1\/135\/1491076\/32953.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,22]],"date-time":"2023-07-22T07:39:43Z","timestamp":1690011583000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jcb\/article\/141\/1\/135\/979\/Apical-Vesicles-Bearing-Inositol-1-4-5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,4,6]]},"references-count":37,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1998,4,6]]}},"URL":"https:\/\/doi.org\/10.1083\/jcb.141.1.135","relation":{},"ISSN":["0021-9525","1540-8140"],"issn-type":[{"value":"0021-9525","type":"print"},{"value":"1540-8140","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,4,6]]}}}