{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T11:29:08Z","timestamp":1791804548354,"version":"4.3.4"},"reference-count":43,"publisher":"Rockefeller University Press","issue":"2","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1998,4,20]]},"abstract":"<jats:p>The nerve axon is a good model system for studying the molecular mechanism of organelle transport in cells. Recently, the new kinesin superfamily proteins (KIFs) have been identified as candidate motor proteins involved in organelle transport. Among them KIF1A, a murine homologue of unc-104 gene of Caenorhabditis elegans, is a unique monomeric neuron\u2013 specific microtubule plus end\u2013directed motor and has been proposed as a transporter of synaptic vesicle precursors (Okada, Y., H. Yamazaki, Y. Sekine-Aizawa, and N. Hirokawa. 1995. Cell. 81:769\u2013780). To elucidate the function of KIF1A in vivo, we disrupted the KIF1A gene in mice. KIF1A mutants died mostly within a day after birth showing motor and sensory disturbances. In the nervous systems of these mutants, the transport of synaptic vesicle precursors showed a specific and significant decrease. Consequently, synaptic vesicle density decreased dramatically, and clusters of clear small vesicles accumulated in the cell bodies. Furthermore, marked neuronal degeneration and death occurred both in KIF1A mutant mice and in cultures of mutant neurons. The neuronal death in cultures was blocked by coculture with wild-type neurons or exposure to a low concentration of glutamate. These results in cultures suggested that the mutant neurons might not sufficiently receive afferent stimulation, such as neuronal contacts or neurotransmission, resulting in cell death. Thus, our results demonstrate that KIF1A transports a synaptic vesicle precursor and that KIF1A-mediated axonal transport plays a critical role in viability, maintenance, and function of neurons, particularly mature neurons.<\/jats:p>","DOI":"10.1083\/jcb.141.2.431","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T12:47:50Z","timestamp":1027687670000},"page":"431-441","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":297,"title":["Defect in Synaptic Vesicle Precursor Transport and Neuronal Cell Death in KIF1A Motor Protein\u2013deficient Mice"],"prefix":"10.1083","volume":"141","author":[{"given":"Yoshiaki","family":"Yonekawa","sequence":"first","affiliation":[{"name":"*Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Tokyo 113, Japan; and \u2021Department of Cell Biology, Cancer Institute, Tokyo 170, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akihiro","family":"Harada","sequence":"additional","affiliation":[{"name":"*Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Tokyo 113, Japan; and \u2021Department of Cell Biology, Cancer Institute, Tokyo 170, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasushi","family":"Okada","sequence":"additional","affiliation":[{"name":"*Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Tokyo 113, Japan; and \u2021Department of Cell Biology, Cancer Institute, Tokyo 170, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeshi","family":"Funakoshi","sequence":"additional","affiliation":[{"name":"*Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Tokyo 113, Japan; and \u2021Department of Cell Biology, Cancer Institute, Tokyo 170, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshimitsu","family":"Kanai","sequence":"additional","affiliation":[{"name":"*Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Tokyo 113, Japan; and \u2021Department of Cell Biology, Cancer Institute, Tokyo 170, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yosuke","family":"Takei","sequence":"additional","affiliation":[{"name":"*Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Tokyo 113, Japan; and \u2021Department of Cell Biology, Cancer Institute, Tokyo 170, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sumio","family":"Terada","sequence":"additional","affiliation":[{"name":"*Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Tokyo 113, Japan; and \u2021Department of Cell Biology, Cancer Institute, Tokyo 170, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsuo","family":"Noda","sequence":"additional","affiliation":[{"name":"*Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Tokyo 113, Japan; and \u2021Department of Cell Biology, Cancer Institute, Tokyo 170, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nobutaka","family":"Hirokawa","sequence":"additional","affiliation":[{"name":"*Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Tokyo 113, Japan; and \u2021Department of Cell Biology, Cancer Institute, Tokyo 170, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1998,4,20]]},"reference":[{"key":"2023072207410384200_B1"},{"key":"2023072207410384200_B2","doi-asserted-by":"crossref","first-page":"1287","DOI":"10.1083\/jcb.119.5.1287","article-title":"Kinesin family in murine central nervous system","volume":"119","author":"Aizawa","year":"1992","journal-title":"J Cell Biol"},{"key":"2023072207410384200_B3","doi-asserted-by":"crossref","first-page":"1954","DOI":"10.1523\/JNEUROSCI.04-08-01954.1984","article-title":"An electron microscopic study of the development of axons and dendrites of hippocampal neurons in culture. II. Synaptic relationships","volume":"4","author":"Barlet","year":"1984","journal-title":"J Neurosci"},{"key":"2023072207410384200_B4","doi-asserted-by":"crossref","first-page":"628","DOI":"10.1016\/0955-0674(93)90132-A","article-title":"Biogenesis of constitutive secretory vesicles, secretory granules and synaptic vesicles","volume":"5","author":"Bauerfeind","year":"1993","journal-title":"Curr Opin Cell Biol"},{"key":"2023072207410384200_B5","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1038\/317073a0","article-title":"A novel brain ATPase with properties expected for the fast axonal transport motor","volume":"317","author":"Brady","year":"1985","journal-title":"Nature"},{"key":"2023072207410384200_B6","doi-asserted-by":"crossref","first-page":"1284","DOI":"10.1083\/jcb.100.4.1284","article-title":"Identification of a transmembrane glycoprotein specific for secretory vesicles of neuronal and endocrine cells","volume":"100","author":"Buckley","year":"1985","journal-title":"J 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