{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T13:27:46Z","timestamp":1791120466785,"version":"4.1.0"},"reference-count":54,"publisher":"Rockefeller University Press","issue":"5","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1998,9,7]]},"abstract":"<jats:p>Previously we found that \u03b13\u03b21 integrin\u2013deficient neonatal mice develop micro-blisters at the epidermal\u2013dermal junction. These micro-blisters were associated with poor basement membrane organization. In the present study we have investigated the effect of \u03b13\u03b21-deficiency on other keratinocyte integrins, actin-associated proteins and F-actin organization. We show that the absence of \u03b13\u03b21 results in an increase in stress fiber formation in keratinocytes grown in culture and at the basal face of the basal keratinocytes of \u03b13-null epidermis. Moreover, we see a higher concentration of actin-associated proteins such as vinculin, talin, and \u03b1-actinin at focal contact sites in the \u03b13-deficient keratinocytes. These changes in focal contact composition were not due to a change in steady-state levels of these proteins, but rather to reorganization due to \u03b13\u03b21 deficiency. Apart from the loss of \u03b13\u03b21 there is no change in expression of the other integrins expressed by the \u03b13-null keratinocytes. However, in functional assays, \u03b13\u03b21 deficiency allows an increase in fibronectin and collagen type IV receptor activities. Thus, our findings provide evidence for a role of \u03b13\u03b21 in regulating stress fiber formation and as a trans-dominant inhibitor of the functions of the other integrins in mouse keratinocytes. These results have potential implications for the regulation of keratinocyte adhesion and migration during wound healing.<\/jats:p>","DOI":"10.1083\/jcb.142.5.1357","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T16:46:23Z","timestamp":1027701983000},"page":"1357-1369","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":169,"title":["Novel Roles for \u03b13\u03b21 Integrin as a Regulator of Cytoskeletal Assembly and as a Trans-dominant Inhibitor of Integrin Receptor Function in Mouse Keratinocytes"],"prefix":"10.1083","volume":"142","author":[{"given":"Kairbaan M.","family":"Hodivala-Dilke","sequence":"first","affiliation":[{"name":"*Howard Hughes Medical Institute, Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139; \u2021Division of Nephrology and Newborn Medicine, Children's Hospital and Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Michael DiPersio","sequence":"additional","affiliation":[{"name":"*Howard Hughes Medical Institute, Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139; \u2021Division of Nephrology and Newborn Medicine, Children's Hospital and Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jordan A.","family":"Kreidberg","sequence":"additional","affiliation":[{"name":"*Howard Hughes Medical Institute, Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139; \u2021Division of Nephrology and Newborn Medicine, Children's Hospital and Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard O.","family":"Hynes","sequence":"additional","affiliation":[{"name":"*Howard Hughes Medical Institute, Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139; \u2021Division of Nephrology and Newborn Medicine, Children's Hospital and Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1998,9,7]]},"reference":[{"key":"2023072900051129400_B1","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1016\/0092-8674(77)90057-5","article-title":"The phagokinetic tracks of 3T3 cells","volume":"11","author":"Albrecht-Buehler","year":"1977","journal-title":"Cell"},{"key":"2023072900051129400_B2","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1002\/ijc.2910580405","article-title":"Transformation and tumor 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