{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T22:47:10Z","timestamp":1792622830840,"version":"4.5.0"},"reference-count":33,"publisher":"Rockefeller University Press","issue":"3","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1998,11,2]]},"abstract":"<jats:p>In the ocular lens, gap junctional communication is a key component of homeostatic mechanisms preventing cataract formation. Gap junctions in rodent lens fibers contain two known intercellular channel-forming proteins, connexin50 (Cx50) and Cx46. Since targeted ablation of Cx46 has been shown to cause senile-type nuclear opacities, it appears that Cx50 alone cannot meet homeostatic requirements. To determine if lens pathology arises from a reduction in levels of communication or the loss of a connexin-specific function, we have generated mice with a targeted deletion of the Cx50 gene. Cx50-null mice exhibited microphthalmia and nuclear cataracts. At postnatal day 14 (P14), Cx50-knockout eyes weighed 32% less than controls, whereas lens mass was reduced by 46%. Cx50-knockout lenses also developed zonular pulverulent cataracts, and lens abnormalities were detected by P7. Deletion of Cx50 did not alter the amounts or distributions of Cx46 or Cx43, a component of lens epithelial junctions. In addition, intercellular passage of tracers revealed the persistence of communication between all cell types in the Cx50-knockout lens. These results demonstrate that Cx50 is required not only for maintenance of lens transparency but also for normal eye growth. Furthermore, these data indicate that unique functional properties of both Cx46 and Cx50 are required for proper lens development.<\/jats:p>","DOI":"10.1083\/jcb.143.3.815","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T16:47:50Z","timestamp":1027702070000},"page":"815-825","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":269,"title":["Targeted Ablation of Connexin50 in Mice Results in Microphthalmia and Zonular Pulverulent Cataracts"],"prefix":"10.1083","volume":"143","author":[{"given":"Thomas W.","family":"White","sequence":"first","affiliation":[{"name":"Department of Cell Biology and Department of Neurobiology, Harvard Medical School, Boston, Massachusetts 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel A.","family":"Goodenough","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Department of Neurobiology, Harvard Medical School, Boston, Massachusetts 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David L.","family":"Paul","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Department of Neurobiology, Harvard Medical School, Boston, Massachusetts 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1998,11,2]]},"reference":[{"key":"2023072900593970300_B1","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1242\/jcs.107.4.799","article-title":"Intercellular communication between epithelial and fiber cells of the eye lens","volume":"107","author":"Basnet","year":"1994","journal-title":"J Cell Sci"},{"key":"2023072900593970300_B2","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1083\/jcb.108.2.595","article-title":"Antisera directed against connexin43 peptides react with a 43-kD protein localized to gap junctions in myocardium and other tissues","volume":"108","author":"Beyer","year":"1989","journal-title":"J Cell Biol"},{"key":"2023072900593970300_B3","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1002\/elps.1150080203","article-title":"Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels","volume":"8","author":"Blum","year":"1987","journal-title":"Electrophoresis"},{"key":"2023072900593970300_B4","doi-asserted-by":"crossref","first-page":"1563","DOI":"10.1126\/science.3685993","article-title":"Genetic ablation: targeted expression of a toxin gene causes micropthalmia in transgenic mice","volume":"238","author":"Breitman","year":"1987","journal-title":"Science"},{"key":"2023072900593970300_B5","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1432-1033.1996.0001q.x","article-title":"Connections with connexins\u2014 the molecular basis of direct intercellular signaling [Review]","volume":"238","author":"Bruzzone","year":"1996","journal-title":"Eur J Biochem"},{"key":"2023072900593970300_B6","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1002\/jez.1401560104","article-title":"Lens development 1. 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