{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T18:20:18Z","timestamp":1790706018726,"version":"4.1.0"},"reference-count":44,"publisher":"Rockefeller University Press","issue":"4","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1998,11,16]]},"abstract":"<jats:p>Shigella flexneri, the causative agent of bacillary dysentery, has the ability to enter nonphagocytic cells. The interferon (IFN) family of cytokines was found to inhibit Shigella invasion of cultured epithelial cells. We show here that IFN-\u03b1 inhibits a Src-dependent signaling cascade triggered by Shigella that leads to the reorganization of the host cell cytoskeleton. Immunofluorescence studies showed that IFN-\u03b1 inhibits Shigella-induced actin polymerization required for bacterial entry into cells. Phosphorylation of cortactin, a Src-substrate specifically tyrosyl-phosphorylated during Shigella entry, was inhibited by IFN-\u03b1. Overexpression of a dominant interfering form of pp60c-src led to inhibition of Shigella-induced cytoskeletal rearrangements and decreased cortactin phosphorylation indicating a role for Src in Shigella entry. Also, Shigella uptake in cells that expressed constitutively active Src was unaffected by IFN-\u03b1 treatment. We conclude that Src kinase activity is necessary for Shigella invasion of epithelial cells and that IFN-\u03b1 inhibits this Src-dependent signaling pathway.<\/jats:p>","DOI":"10.1083\/jcb.143.4.1003","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T16:47:50Z","timestamp":1027702070000},"page":"1003-1012","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":54,"title":["Interferon \u03b1 Inhibits a Src-mediated Pathway Necessary for <i>Shigella<\/i>-induced Cytoskeletal Rearrangements in Epithelial Cells"],"prefix":"10.1083","volume":"143","author":[{"given":"G.","family":"Dum\u00e9nil","sequence":"first","affiliation":[{"name":"*Unit\u00e9 de G\u00e9n\u00e9tique Humaine, INSERM U276 and \u2021Unit\u00e9 de Pathog\u00e9nie Microbienne Mol\u00e9culaire, INSERM U389, Institut Pasteur, 75724 Paris Cedex 15, France; and \u2016European Molecular Biology Laboratory, Cell Biophysics Program, D-69117 Heidelberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.C.","family":"Olivo","sequence":"additional","affiliation":[{"name":"*Unit\u00e9 de G\u00e9n\u00e9tique Humaine, INSERM U276 and \u2021Unit\u00e9 de Pathog\u00e9nie Microbienne Mol\u00e9culaire, INSERM U389, Institut Pasteur, 75724 Paris Cedex 15, France; and \u2016European Molecular Biology Laboratory, Cell Biophysics Program, D-69117 Heidelberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Pellegrini","sequence":"additional","affiliation":[{"name":"*Unit\u00e9 de G\u00e9n\u00e9tique Humaine, INSERM U276 and \u2021Unit\u00e9 de Pathog\u00e9nie Microbienne Mol\u00e9culaire, INSERM U389, Institut Pasteur, 75724 Paris Cedex 15, France; and \u2016European Molecular Biology Laboratory, Cell Biophysics Program, D-69117 Heidelberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Fellous","sequence":"additional","affiliation":[{"name":"*Unit\u00e9 de G\u00e9n\u00e9tique Humaine, INSERM U276 and \u2021Unit\u00e9 de Pathog\u00e9nie Microbienne Mol\u00e9culaire, INSERM U389, Institut Pasteur, 75724 Paris Cedex 15, France; and \u2016European Molecular Biology Laboratory, Cell Biophysics Program, D-69117 Heidelberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.J.","family":"Sansonetti","sequence":"additional","affiliation":[{"name":"*Unit\u00e9 de G\u00e9n\u00e9tique Humaine, INSERM U276 and \u2021Unit\u00e9 de Pathog\u00e9nie Microbienne Mol\u00e9culaire, INSERM U389, Institut Pasteur, 75724 Paris Cedex 15, France; and \u2016European Molecular Biology Laboratory, Cell Biophysics Program, D-69117 Heidelberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. 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