{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T20:50:24Z","timestamp":1791492624856,"version":"4.3.4"},"reference-count":50,"publisher":"Rockefeller University Press","issue":"6","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1998,12,14]]},"abstract":"<jats:p>Laminins are the major noncollagenous glycoproteins of all basal laminae (BLs). They are \u03b1\/\u03b2\/\u03b3 heterotrimers assembled from 10 known chains, and they subserve both structural and signaling roles. Previously described mutations in laminin chain genes result in diverse disorders that are manifested postnatally and therefore provide little insight into laminin's roles in embryonic development. Here, we show that the laminin \u03b15 chain is required during embryogenesis. The \u03b15 chain is present in virtually all BLs of early somite stage embryos and then becomes restricted to specific BLs as development proceeds, including those of the surface ectoderm and placental vasculature. BLs that lose \u03b15 retain or acquire other \u03b1 chains. Embryos lacking laminin \u03b15 die late in embryogenesis. They exhibit multiple developmental defects, including failure of anterior neural tube closure (exencephaly), failure of digit septation (syndactyly), and dysmorphogenesis of the placental labyrinth. These defects are all attributable to defects in BLs that are \u03b15 positive in controls and that appear ultrastructurally abnormal in its absence. Other laminin \u03b1 chains accumulate in these BLs, but this compensation is apparently functionally inadequate. Our results identify new roles for laminins and BLs in diverse developmental processes.<\/jats:p>","DOI":"10.1083\/jcb.143.6.1713","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T12:45:41Z","timestamp":1027687541000},"page":"1713-1723","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":418,"title":["Roles for Laminin in Embryogenesis: Exencephaly, Syndactyly, and Placentopathy in Mice Lacking the Laminin \u03b15 Chain"],"prefix":"10.1083","volume":"143","author":[{"given":"Jeffrey H.","family":"Miner","sequence":"first","affiliation":[{"name":"*Department of Medicine, Renal Division and \u2021Department of Anatomy and Neurobiology, Washington University School of Medicine, St. Louis, Missouri 63110"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeanette","family":"Cunningham","sequence":"additional","affiliation":[{"name":"*Department of Medicine, Renal Division and \u2021Department of Anatomy and Neurobiology, Washington University School of Medicine, St. Louis, Missouri 63110"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua R.","family":"Sanes","sequence":"additional","affiliation":[{"name":"*Department of Medicine, Renal Division and \u2021Department of Anatomy and Neurobiology, Washington University School of Medicine, St. Louis, Missouri 63110"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1998,12,14]]},"reference":[{"key":"2023072901014784900_B1","doi-asserted-by":"crossref","first-page":"115","DOI":"10.3109\/15419069409004431","article-title":"Developmental expression of nicein adhesion protein (laminin-5) subunits suggests multiple morphogenic roles","volume":"2","author":"Aberdam","year":"1994","journal-title":"Cell Adhes 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