{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T08:11:14Z","timestamp":1779178274768,"version":"3.51.4"},"reference-count":39,"publisher":"Rockefeller University Press","issue":"1","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1999,7,12]]},"abstract":"<jats:p>Neuropilin-1 (NRP1) is a receptor for two unrelated ligands with disparate activities, vascular endothelial growth factor-165 (VEGF165), an angiogenesis factor, and semaphorin\/collapsins, mediators of neuronal guidance. To determine whether semaphorin\/collapsins could interact with NRP1 in nonneuronal cells, the effects of recombinant collapsin-1 on endothelial cells (EC) were examined. Collapsin-1 inhibited the motility of porcine aortic EC (PAEC) expressing NRP1 alone; coexpressing KDR and NRP1 (PAEC\/KDR\/NRP1), but not parental PAEC; or PAEC expressing KDR alone. The motility of PAEC expressing NRP1 was inhibited by 65\u201375% and this inhibition was abrogated by anti-NRP1 antibody. In contrast, VEGF165 stimulated the motility of PAEC\/KDR\/NRP1. When VEGF165 and collapsin-1 were added simultaneously to PAEC\/KDR\/NRP1, dorsal root ganglia (DRG), and COS-7\/NRP1 cells, they competed with each other in EC motility, DRG collapse, and NRP1-binding assays, respectively, suggesting that the two ligands have overlapping NRP1 binding sites. Collapsin-1 rapidly disrupted the formation of lamellipodia and induced depolymerization of F-actin in an NRP1-dependent manner. In an in vitro angiogenesis assay, collapsin-1 inhibited the capillary sprouting of EC from rat aortic ring segments. These results suggest that collapsin-1 can inhibit EC motility as well as axon motility, that these inhibitory effects on motility are mediated by NRP1, and that VEGF165 and collapsin-1 compete for NRP1-binding sites.<\/jats:p>","DOI":"10.1083\/jcb.146.1.233","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T16:45:41Z","timestamp":1027701941000},"page":"233-242","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":190,"title":["Neuropilin-1 Mediates Collapsin-1\/Semaphorin III Inhibition of Endothelial Cell Motility"],"prefix":"10.1083","volume":"146","author":[{"given":"Hua-Quan","family":"Miao","sequence":"first","affiliation":[{"name":"aDepartment of Surgical Research, Children's Hospital and Harvard Medical School, Boston, Massachusetts 02115"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shay","family":"Soker","sequence":"additional","affiliation":[{"name":"aDepartment of Surgical Research, Children's Hospital and Harvard Medical School, Boston, Massachusetts 02115"},{"name":"cDepartment of Urology, Children's Hospital and Harvard Medical School, Boston, Massachusetts 02115"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leonard","family":"Feiner","sequence":"additional","affiliation":[{"name":"dDepartment of Neuroscience, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Luis","family":"Alonso","sequence":"additional","affiliation":[{"name":"aDepartment of Surgical Research, Children's Hospital and Harvard Medical School, Boston, Massachusetts 02115"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonathan A.","family":"Raper","sequence":"additional","affiliation":[{"name":"dDepartment of Neuroscience, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Klagsbrun","sequence":"additional","affiliation":[{"name":"aDepartment of Surgical Research, Children's Hospital and Harvard Medical School, Boston, Massachusetts 02115"},{"name":"bDepartment of Pathology, Children's Hospital and Harvard Medical School, Boston, Massachusetts 02115"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[1999,7,12]]},"reference":[{"key":"2023072900402349900_Beharetal1996","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1038\/383525a0","article-title":"Semaphorin III is needed for normal patterning and growth of nerves, bones and heart","volume":"383","author":"Behar","year":"1996","journal-title":"Nature."},{"key":"2023072900402349900_Eleniusetal1997","doi-asserted-by":"crossref","first-page":"1268","DOI":"10.1093\/emboj\/16.6.1268","article-title":"Activation of HER4 by heparin-binding EGF-like growth factor stimulates chemotaxis but not proliferation","volume":"16","author":"Elenius","year":"1997","journal-title":"EMBO (Eur. 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