{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T17:29:47Z","timestamp":1791307787536,"version":"4.3.1"},"reference-count":29,"publisher":"Rockefeller University Press","issue":"2","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1999,7,26]]},"abstract":"<jats:p>Cryo-electron microscopy and three-dimensional, single-particle image analysis have been used to reveal the specific binding site of imperatoxin A (IpTxa) on the architecture of the calcium release channel\/ryanodine receptor from skeletal muscle (RyR1). IpTxa is a peptide toxin that binds with high affinity to RyR1 and affects its functioning. The toxin was derivatized with biotin to enhance its detection with streptavidin. IpTxa binds to the cytoplasmic moiety of RyR1 between the clamp and handle domains, 11 nm away from the transmembrane pore. The proposed mimicry by IpTxa of the dihydropyridine receptor (DHPR) II-III loop, thought to be a main physiological excitation-contraction trigger, suggests that the IpTxa binding location is a potential excitation-contraction signal transduction site.<\/jats:p>","DOI":"10.1083\/jcb.146.2.493","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T16:45:41Z","timestamp":1027701941000},"page":"493-500","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":57,"title":["Three-Dimensional Location of the Imperatoxin a Binding Site on the Ryanodine Receptor"],"prefix":"10.1083","volume":"146","author":[{"given":"Montserrat","family":"Sams\u00f3","sequence":"first","affiliation":[{"name":"aDivision of Molecular Medicine, Wadsworth Center, Albany, New York 12201-0509"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramon","family":"Trujillo","sequence":"additional","affiliation":[{"name":"aDivision of Molecular Medicine, Wadsworth Center, Albany, New York 12201-0509"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georgina B.","family":"Gurrola","sequence":"additional","affiliation":[{"name":"bDepartment of Physiology, University of Wisconsin, Madison, Wisconsin 53706"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hector H.","family":"Valdivia","sequence":"additional","affiliation":[{"name":"bDepartment of Physiology, University of Wisconsin, Madison, Wisconsin 53706"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Terence","family":"Wagenknecht","sequence":"additional","affiliation":[{"name":"aDivision of Molecular Medicine, Wadsworth Center, Albany, New York 12201-0509"},{"name":"cDepartment of Biomedical Sciences, State University of New York, Albany, New York 12201"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1999,7,26]]},"reference":[{"key":"2023072901243644000_Blocketal1988","doi-asserted-by":"crossref","first-page":"2587","DOI":"10.1083\/jcb.107.6.2587","article-title":"Structural evidence for direct interaction between the molecular components of the transverse tubule\/sarcoplasmic reticulum junction in skeletal muscle","volume":"107","author":"Block","year":"1988","journal-title":"J. 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