{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T05:52:20Z","timestamp":1791352340382,"version":"4.3.1"},"reference-count":42,"publisher":"Rockefeller University Press","issue":"1","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1999,10,4]]},"abstract":"<jats:p>Claudins, comprising a multigene family, constitute tight junction (TJ) strands. Clostridium perfringens enterotoxin (CPE), a single \u223c35-kD polypeptide, was reported to specifically bind to claudin-3\/RVP1 and claudin-4\/CPE-R at its COOH-terminal half. We examined the effects of the COOH-terminal half fragment of CPE (C-CPE) on TJs in L transfectants expressing claudin-1 to -4 (C1L to C4L, respectively), and in MDCK I cells expressing claudin-1 and -4. C-CPE bound to claudin-3 and -4 with high affinity, but not to claudin-1 or -2. In the presence of C-CPE, reconstituted TJ strands in C3L cells gradually disintegrated and disappeared from their cell surface. In MDCK I cells incubated with C-CPE, claudin-4 was selectively removed from TJs with its concomitant degradation. At 4 h after incubation with C-CPE, TJ strands were disintegrated, and the number of TJ strands and the complexity of their network were markedly decreased. In good agreement with the time course of these morphological changes, the TJ barrier (TER and paracellular flux) of MDCK I cells was downregulated by C-CPE in a dose-dependent manner. These findings provided evidence for the direct involvement of claudins in the barrier functions of TJs.<\/jats:p>","DOI":"10.1083\/jcb.147.1.195","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T12:45:41Z","timestamp":1027687541000},"page":"195-204","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":556,"title":["<i>Clostridium perfringens<\/i>\n                    Enterotoxin Fragment Removes Specific Claudins from Tight Junction Strands"],"prefix":"10.1083","volume":"147","author":[{"given":"Noriyuki","family":"Sonoda","sequence":"first","affiliation":[{"name":"aDepartment of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mikio","family":"Furuse","sequence":"additional","affiliation":[{"name":"aDepartment of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroyuki","family":"Sasaki","sequence":"additional","affiliation":[{"name":"cLaboratory of Cell Biology, KAN Research Institute Inc., Kyoto Research Park, Chudoji, Shimogyo-ku, Kyoto 600-8317, Japan"},{"name":"dDepartment of Molecular Cell Biology, Institute of DNA Medicine, The Jikei University School of Medicine, Nishi-Shinbashi, Minato-ku, Tokyo 105-8461, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigenobu","family":"Yonemura","sequence":"additional","affiliation":[{"name":"aDepartment of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Katahira","sequence":"additional","affiliation":[{"name":"bProject Research for Molecular Bacteriology, Research Institute for Microbial Diseases, Osaka University 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuhiko","family":"Horiguchi","sequence":"additional","affiliation":[{"name":"bProject Research for Molecular Bacteriology, Research Institute for Microbial Diseases, Osaka University 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shoichiro","family":"Tsukita","sequence":"additional","affiliation":[{"name":"aDepartment of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1999,10,4]]},"reference":[{"key":"2023072900575539000_AndersonandvanItallie1995","first-page":"G467","article-title":"Tight junctions and the molecular basis for regulation of paracellular permeability","volume":"269","author":"Anderson","year":"1995","journal-title":"Am. 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