{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,23]],"date-time":"2025-08-23T04:58:59Z","timestamp":1755925139324},"reference-count":39,"publisher":"Rockefeller University Press","issue":"6","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1999,12,13]]},"abstract":"<jats:p>The variant chicken kidney AE1 anion exchangers differ only at the NH2 terminus of their cytoplasmic domains. Transfection studies have indicated that the variant chicken AE1-4 anion exchanger accumulates in the basolateral membrane of polarized MDCK kidney epithelial cells, while the AE1-3 variant, which lacks the NH2-terminal 63 amino acids of AE1-4, primarily accumulates in the apical membrane. Mutagenesis studies have shown that the basolateral accumulation of AE1-4 is dependent upon two tyrosine residues at amino acids 44 and 47 of the polypeptide. Interestingly, either of these tyrosines is sufficient to direct efficient basolateral sorting of AE1-4. However, in the absence of both tyrosine residues, AE1-4 accumulates in the apical membrane of MDCK cells. Pulse\u2013chase studies have shown that after delivery to the cell surface, newly synthesized AE1-4 is recycled to the Golgi where it acquires additional N-linked sugar modifications. This Golgi recycling activity is dependent upon the same cytoplasmic tyrosine residues that are required for the basolateral sorting of this variant transporter. Furthermore, mutants of AE1-4 that are defective in Golgi recycling are unable to associate with the detergent insoluble actin cytoskeleton and are rapidly turned over. These studies, which represent the first description of tyrosine-dependent cytoplasmic sorting signal for a type III membrane protein, have suggested a critical role for the actin cytoskeleton in regulating AE1 anion exchanger localization and stability in this epithelial cell type.<\/jats:p>","DOI":"10.1083\/jcb.147.6.1237","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T16:45:41Z","timestamp":1027701941000},"page":"1237-1248","update-policy":"http:\/\/dx.doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":19,"title":["Intracellular Trafficking of Variant Chicken Kidney Ae1 Anion Exchangers"],"prefix":"10.1083","volume":"147","author":[{"given":"Tracy L.","family":"Adair-Kirk","sequence":"first","affiliation":[{"name":"aDepartment of Microbiology and Immunology, University of Tennessee Health Science Center, 858 Madison Avenue, Memphis, Tennessee 38163"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kathleen H.","family":"Cox","sequence":"additional","affiliation":[{"name":"aDepartment of Microbiology and Immunology, University of Tennessee Health Science Center, 858 Madison Avenue, Memphis, Tennessee 38163"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John V.","family":"Cox","sequence":"additional","affiliation":[{"name":"aDepartment of Microbiology and Immunology, University of Tennessee Health Science Center, 858 Madison Avenue, Memphis, Tennessee 38163"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[1999,12,13]]},"reference":[{"key":"2023072901270655700_Aguilaretal1997","doi-asserted-by":"crossref","first-page":"27160","DOI":"10.1074\/jbc.272.43.27160","article-title":"Functional domain mapping of the clathrin-associated adaptor medium chains mu1 and mu2","volume":"272","author":"Aguilar","year":"1997","journal-title":"J. Biol. Chem."},{"key":"2023072901270655700_Alperetal1989","doi-asserted-by":"crossref","first-page":"5429","DOI":"10.1073\/pnas.86.14.5429","article-title":"Subtypes of intercalated cells in rat kidney collecting duct defined by antibodies against erythroid band 3 and renal vacuolar H+-ATPase","volume":"86","author":"Alper","year":"1989","journal-title":"Proc. Natl. Acad. Sci. USA."},{"key":"2023072901270655700_Bolletal1996","doi-asserted-by":"crossref","first-page":"5789","DOI":"10.1002\/j.1460-2075.1996.tb00965.x","article-title":"Sequence requirements for the recognition of tyrosine-based endocytic signals by clathrin AP-2 complexes","volume":"15","author":"Boll","year":"1996","journal-title":"EMBO (Eur. Mol. Biol. Organ.) J."},{"key":"2023072901270655700_Brosiusetal1989","doi-asserted-by":"crossref","first-page":"7784","DOI":"10.1016\/S0021-9258(18)83108-8","article-title":"The major kidney band 3 gene transcript predicts an amino-terminal truncated band 3 polypeptide","volume":"264","author":"Brosius","year":"1989","journal-title":"J. Biol. Chem."},{"key":"2023072901270655700_Casanovaetal1991","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/0092-8674(91)90139-P","article-title":"An autonomous signal for basolateral sorting in the cytoplasmic domain of the polymeric immunoglobulin receptor","volume":"66","author":"Casanova","year":"1991","journal-title":"Cell"},{"key":"2023072901270655700_ChapmanandMunro1994","doi-asserted-by":"crossref","first-page":"2305","DOI":"10.1002\/j.1460-2075.1994.tb06514.x","article-title":"Retrieval of TGN proteins from the cell surface requires endosomal acidification","volume":"13","author":"Chapman","year":"1994","journal-title":"EMBO (Eur. Mol. Biol. Organ.) J."},{"key":"2023072901270655700_Collawnetal1990","doi-asserted-by":"crossref","first-page":"1061","DOI":"10.1016\/0092-8674(90)90509-D","article-title":"Transferrin receptor internalization sequence YXRF implicates a tight turn as the structural recognition motif for endocytosis","volume":"63","author":"Collawn","year":"1990","journal-title":"Cell"},{"key":"2023072901270655700_Coxetal1995","doi-asserted-by":"crossref","first-page":"19752","DOI":"10.1074\/jbc.270.34.19752","article-title":"Four variant chicken erythroid AE1 anion exchangers. Role of the alternative N-terminal sequences in intracellular targeting in transfected human erythroleukemia cells","volume":"270","author":"Cox","year":"1995","journal-title":"J. Biol. Chem."},{"key":"2023072901270655700_Coxetal1996","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/0378-1119(96)00211-9","article-title":"Variant chicken kidney AE1 anion exchanger transcripts are derived from a single promoter by alternative splicing","volume":"173","author":"Cox","year":"1996","journal-title":"Gene."},{"key":"2023072901270655700_CoxandCox1995","first-page":"F503","article-title":"Variant chicken AE1 anion exchangers possess divergent NH(2)-terminal cytoplasmic domains","volume":"268","author":"Cox","year":"1995","journal-title":"Am. J. Physiol."},{"key":"2023072901270655700_DellAngelicaetal1997","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1093\/emboj\/16.5.917","article-title":"AP-3an adaptor-like protein complex with ubiquitous expression","volume":"16","author":"Dell'Angelica","year":"1997","journal-title":"EMBO (Eur. Mol. Biol. Organ.) J."},{"key":"2023072901270655700_Dingetal1994","doi-asserted-by":"crossref","first-page":"32201","DOI":"10.1016\/S0021-9258(18)31621-1","article-title":"The major kidney AE1 isoform does not bind ankyrin (ANK1) in vitro","volume":"269","author":"Ding","year":"1994","journal-title":"J. Biol. Chem."},{"key":"2023072901270655700_Drenckhahnetal1985","doi-asserted-by":"crossref","first-page":"1287","DOI":"10.1126\/science.2933809","article-title":"Colocalization of band 3 with ankyrin and spectrin at the basal membrane of intercalated cells in the rat kidney","volume":"230","author":"Drenckhahn","year":"1985","journal-title":"Science"},{"key":"2023072901270655700_Eberleetal1991","doi-asserted-by":"crossref","first-page":"1203","DOI":"10.1016\/0092-8674(91)90296-B","article-title":"The essential tyrosine of the internalization signal in lysosomal acid phosphatase is part of a beta turn","volume":"67","author":"Eberle","year":"1991","journal-title":"Cell"},{"key":"2023072901270655700_FiedlerandSimons1995","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1016\/0092-8674(95)90380-1","article-title":"The role of N-glycans in the secretory pathway","volume":"81","author":"Fiedler","year":"1995","journal-title":"Cell"},{"key":"2023072901270655700_Ghoshetal1999","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1091\/mbc.10.2.455","article-title":"Chicken erythroid AE1 anion exchangers associate with the cytoskeleton during recycling to the Golgi","volume":"10","author":"Ghosh","year":"1999","journal-title":"Mol. Biol. Cell."},{"key":"2023072901270655700_Hansenetal1993","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1083\/jcb.121.1.61","article-title":"Clathrin and HA2 adaptorseffects of potassium depletion, hypertonic medium, and cytosol acidification","volume":"121","author":"Hansen","year":"1993","journal-title":"J. Cell Biol."},{"key":"2023072901270655700_HoningandHunziker1995","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1083\/jcb.128.3.321","article-title":"Cytoplasmic determinants involved in direct lysosomal sorting, endocytosis, and basolateral targeting of rat lgp120 (lamp-I) in MDCK cells","volume":"128","author":"Honing","year":"1995","journal-title":"J. Cell Biol."},{"key":"2023072901270655700_HunzikerandFumey1994","doi-asserted-by":"crossref","first-page":"2963","DOI":"10.1002\/j.1460-2075.1994.tb06594.x","article-title":"A di-leucine motif mediates endocytosis and basolateral sorting of macrophage IgG Fc receptors in MDCK cells","volume":"13","author":"Hunziker","year":"1994","journal-title":"EMBO (Eur. Mol. Biol. Organ.) J."},{"key":"2023072901270655700_Kollert-Jonsetal1993","first-page":"F813","article-title":"Anion exchanger 1 in human kidney and oncocytoma differs from erythroid AE1 in its NH2 terminus","volume":"265","author":"Kollert-Jons","year":"1993","journal-title":"Am. J. Physiol."},{"key":"2023072901270655700_Kozak1986","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/0092-8674(86)90762-2","article-title":"Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes","volume":"44","author":"Kozak","year":"1986","journal-title":"Cell"},{"key":"2023072901270655700_KudryckiandShull1989","doi-asserted-by":"crossref","first-page":"8185","DOI":"10.1016\/S0021-9258(18)83167-2","article-title":"Primary structure of the rat kidney band 3 anion exchange protein deduced from a cDNA","volume":"264","author":"Kudrycki","year":"1989","journal-title":"J. Biol. Chem."},{"key":"2023072901270655700_Matteretal1992","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1016\/0092-8674(92)90551-M","article-title":"Basolateral sorting of LDL receptor in MDCK cellsthe cytoplasmic domain contains two tyrosine-dependent targeting determinants","volume":"71","author":"Matter","year":"1992","journal-title":"Cell"},{"key":"2023072901270655700_Muschetal1999","doi-asserted-by":"crossref","first-page":"7923","DOI":"10.1074\/jbc.274.12.7923","article-title":"Volume expansion stimulates p72(syk) and p56(lyn) in skate erythrocytes","volume":"274","author":"Musch","year":"1999","journal-title":"J. Biol. Chem."},{"key":"2023072901270655700_NaimandRoth1994","doi-asserted-by":"crossref","first-page":"3928","DOI":"10.1016\/S0021-9258(17)41722-4","article-title":"Characteristics of the internalization signal in the Y543 influenza virus hemagglutinin suggest a model for recognition of internalization signals containing tyrosine","volume":"269","author":"Naim","year":"1994","journal-title":"J. Biol. Chem."},{"key":"2023072901270655700_NelsonandHammerton1989","doi-asserted-by":"crossref","first-page":"893","DOI":"10.1083\/jcb.108.3.893","article-title":"A membrane cytoskeletal complex containing Na+,K+-ATPase, ankyrin, and fodrin in MDCK cellsImplications for the biogenesis of epithelial cell polarity","volume":"108","author":"Nelson","year":"1989","journal-title":"J. Cell Biol."},{"key":"2023072901270655700_Ohnoetal1998","doi-asserted-by":"crossref","first-page":"25915","DOI":"10.1074\/jbc.273.40.25915","article-title":"The medium subunits of adaptor complexes recognize distinct but overlapping sets of tyrosine-based sorting signals","volume":"273","author":"Ohno","year":"1998","journal-title":"J. Biol. Chem."},{"key":"2023072901270655700_Ohnoetal1996","doi-asserted-by":"crossref","first-page":"29009","DOI":"10.1074\/jbc.271.46.29009","article-title":"Structural determinants of interaction of tyrosine-based sorting signals with the adaptor medium chains","volume":"271","author":"Ohno","year":"1996","journal-title":"J. Biol. Chem."},{"key":"2023072901270655700_Petersetal1995","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1083\/jcb.130.2.313","article-title":"Ank3 (epithelial ankyrin), a widely distributed new member of the ankyrin gene family and the major ankyrin in kidney, is expressed in alternatively spliced forms, including forms that lack the repeat domain","volume":"130","author":"Peters","year":"1995","journal-title":"J. Cell Biol"},{"key":"2023072901270655700_Petersetal1996","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1016\/S0092-8674(00)80167-1","article-title":"Anion exchanger 1 (band 3) is required to prevent erythrocyte membrane surface loss but not to form the membrane skeleton","volume":"86","author":"Peters","year":"1996","journal-title":"Cell"},{"key":"2023072901270655700_Schaferetal1995","doi-asserted-by":"crossref","first-page":"2424","DOI":"10.1002\/j.1460-2075.1995.tb07240.x","article-title":"Two independent targeting signals in the cytoplasmic domain determine trans-Golgi network localization and endosomal trafficking of the proprotein convertase furin","volume":"14","author":"Schafer","year":"1995","journal-title":"EMBO (Eur. Mol. Biol. Organ.) J."},{"key":"2023072901270655700_Scheiffeleetal1995","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1038\/378096a0","article-title":"N-glycans as apical sorting signals in epithelial cells","volume":"378","author":"Scheiffele","year":"1995","journal-title":"Nature"},{"key":"2023072901270655700_Schwartzetal1985","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1038\/318368a0","article-title":"Plasticity of functional epithelial polarity","volume":"318","author":"Schwartz","year":"1985","journal-title":"Nature."},{"key":"2023072901270655700_Stoorgoveletal1996","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1083\/jcb.132.1.21","article-title":"A novel class of clathrin-coated vesicles budding from endosomes","volume":"132","author":"Stoorgovel","year":"1996","journal-title":"J. Cell Biol"},{"key":"2023072901270655700_Thomasetal1993","doi-asserted-by":"crossref","first-page":"3313","DOI":"10.1016\/S0021-9258(18)53695-4","article-title":"Vesicular stomatitis virus glycoprotein contains a dominant cytoplasmic basolateral sorting signal critically dependent upon a tyrosine","volume":"268","author":"Thomas","year":"1993","journal-title":"J. Biol. Chem."},{"key":"2023072901270655700_vanAdelsbergetal1993","doi-asserted-by":"crossref","first-page":"11283","DOI":"10.1016\/S0021-9258(18)82122-6","article-title":"The apical Cl\/HCO3 exchanger of beta intercalated cells","volume":"268","author":"van Adelsberg","year":"1993","journal-title":"J. Biol. Chem."},{"key":"2023072901270655700_Wangetal1995","doi-asserted-by":"crossref","first-page":"17892","DOI":"10.1074\/jbc.270.30.17892","article-title":"Partial characterization of the cytoplasmic domain of human kidney band 3","volume":"270","author":"Wang","year":"1995","journal-title":"J. Biol. Chem."},{"key":"2023072901270655700_WongandHong1993","doi-asserted-by":"crossref","first-page":"22853","DOI":"10.1016\/S0021-9258(18)41606-7","article-title":"The SXYQRL sequence in the cytoplasmic domain of TGN38 plays a major role in trans-Golgi network localization","volume":"268","author":"Wong","year":"1993","journal-title":"J. Biol. Chem."},{"key":"2023072901270655700_Yeamanetal1997","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1083\/jcb.139.4.929","article-title":"The O-glycosylated stalk domain is required for apical sorting of neurotrophin receptors in polarized MDCK cells","volume":"139","author":"Yeaman","year":"1997","journal-title":"J. Cell Biol."}],"container-title":["The Journal of Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/147\/6\/1237\/1854186\/9908093.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/147\/6\/1237\/1854186\/9908093.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,29]],"date-time":"2023-07-29T01:27:46Z","timestamp":1690594066000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jcb\/article\/147\/6\/1237\/46187\/Intracellular-Trafficking-of-Variant-Chicken"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,12,13]]},"references-count":39,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1999,12,13]]}},"URL":"https:\/\/doi.org\/10.1083\/jcb.147.6.1237","relation":{},"ISSN":["0021-9525","1540-8140"],"issn-type":[{"value":"0021-9525","type":"print"},{"value":"1540-8140","type":"electronic"}],"subject":[],"published":{"date-parts":[[1999,12,13]]}}}