{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T07:35:55Z","timestamp":1791272155706,"version":"4.1.0"},"reference-count":35,"publisher":"Rockefeller University Press","issue":"6","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1999,12,13]]},"abstract":"<jats:p>Actin capping protein (CP) binds barbed ends of actin filaments to regulate actin assembly. CP is an \u03b1\/\u03b2 heterodimer. Vertebrates have conserved isoforms of each subunit. Muscle cells contain two \u03b2 isoforms. \u03b21 is at the Z-line; \u03b22 is at the intercalated disc and cell periphery in general. To investigate the functions of the isoforms, we replaced one isoform with another using expression in hearts of transgenic mice.<\/jats:p>\n               <jats:p>Mice expressing \u03b22 had a severe phenotype with juvenile lethality. Myofibril architecture was severely disrupted. The \u03b22 did not localize to the Z-line. Therefore, \u03b21 has a distinct function that includes interactions at the Z-line. Mice expressing \u03b21 showed altered morphology of the intercalated disc, without the lethality or myofibril disruption of the \u03b22-expressing mice.<\/jats:p>\n               <jats:p>The in vivo function of CP is presumed to involve binding barbed ends of actin filaments. To test this hypothesis, we expressed a \u03b21 mutant that poorly binds actin. These mice showed both myofibril disruption and intercalated disc remodeling, as predicted.<\/jats:p>\n               <jats:p>Therefore, CP\u03b21 and CP\u03b22 each have a distinct function that cannot be provided by the other isoform. CP\u03b21 attaches actin filaments to the Z-line, and CP\u03b22 organizes the actin at the intercalated discs.<\/jats:p>","DOI":"10.1083\/jcb.147.6.1287","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T16:45:41Z","timestamp":1027701941000},"page":"1287-1298","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":67,"title":["Vertebrate Isoforms of Actin Capping Protein \u03b2 Have Distinct Functions in Vivo"],"prefix":"10.1083","volume":"147","author":[{"given":"Marilyn C.","family":"Hart","sequence":"first","affiliation":[{"name":"aDepartment of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, Missouri 63110"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John A.","family":"Cooper","sequence":"additional","affiliation":[{"name":"aDepartment of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, Missouri 63110"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1999,12,13]]},"reference":[{"key":"2023072901474408500_Amatrudaetal1990","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1038\/344352a0","article-title":"Disruption of the actin cytoskeleton in yeast capping protein mutants","volume":"344","author":"Amatruda","year":"1990","journal-title":"Nature."},{"key":"2023072901474408500_Amatrudaetal1992","doi-asserted-by":"crossref","first-page":"1151","DOI":"10.1083\/jcb.119.5.1151","article-title":"Effects of null mutations and overexpression of capping protein on morphogenesis, actin distribution, and polarized secretion in yeast","volume":"119","author":"Amatruda","year":"1992","journal-title":"J. Cell Biol."},{"key":"2023072901474408500_Barron-Casellaetal1995","doi-asserted-by":"crossref","first-page":"21472","DOI":"10.1074\/jbc.270.37.21472","article-title":"Sequence analysis and chromosomal localization of human Cap Z. Conserved residues within the actin-binding domain may link Cap Z to gelsolin\/severin and profilin protein families","volume":"270","author":"Barron-Casella","year":"1995","journal-title":"J. Biol. Chem."},{"key":"2023072901474408500_Bonneetal1995","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1038\/ng1295-438","article-title":"Cardiac myosin binding protein-C gene splice acceptor site mutation is associated with familial hypertrophic cardiomyopathy","volume":"11","author":"Bonne","year":"1995","journal-title":"Nat. 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Cytoskelet."},{"key":"2023072901474408500_Hopmannetal1996","doi-asserted-by":"crossref","first-page":"1293","DOI":"10.1083\/jcb.133.6.1293","article-title":"Actin organization, bristle morphology, and viability are affected by actin capping protein mutations in Drosophila","volume":"133","author":"Hopmann","year":"1996","journal-title":"J. Cell Biol."},{"key":"2023072901474408500_Hugetal1992","doi-asserted-by":"crossref","first-page":"923","DOI":"10.1083\/jcb.116.4.923","article-title":"Identification and characterization of an actin-binding site of CapZ","volume":"116","author":"Hug","year":"1992","journal-title":"J. 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Genet."},{"key":"2023072901474408500_Mogensenetal1999","doi-asserted-by":"crossref","first-page":"R39","DOI":"10.1172\/JCI6460","article-title":"Alpha-cardiac actin is a novel disease gene in familial hypertrophic cardiomyopathy","volume":"103","author":"Mogensen","year":"1999","journal-title":"J. Clin. Invest."},{"key":"2023072901474408500_Olsonetal1998","doi-asserted-by":"crossref","first-page":"750","DOI":"10.1126\/science.280.5364.750","article-title":"Actin mutations in dilated cardiomyopathy, a heritable form of heart failure","volume":"280","author":"Olson","year":"1998","journal-title":"Science."},{"key":"2023072901474408500_Palermoetal1995","first-page":"501","article-title":"Remodeling the mammalian heart using transgenesis","volume":"41","author":"Palermo","year":"1995","journal-title":"Cell. Mol. Biol. Res."},{"key":"2023072901474408500_Palermoetal1996","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1161\/01.RES.78.3.504","article-title":"Transgenic remodeling of the contractile apparatus in the mammalian heart","volume":"78","author":"Palermo","year":"1996","journal-title":"Circ. Res."},{"key":"2023072901474408500_Papaetal1999","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1023\/A:1005489319058","article-title":"Alpha actinin\u2013CapZ, an anchoring complex for thin filaments in Z-line","volume":"20","author":"Papa","year":"1999","journal-title":"J. Muscle Res. Cell Motil."},{"key":"2023072901474408500_Pierobonetal1989","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1007\/BF01771820","article-title":"The organization of titin (connectin) and nebulin in the sarcomeresan immunocytolocalization study","volume":"10","author":"Pierobon","year":"1989","journal-title":"J. Muscle. Res. 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Cytoskelet."},{"key":"2023072901474408500_Seppetal1996","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1136\/hrt.76.5.412","article-title":"Altered patterns of cardiac intercellular junction distribution in hypertrophic cardiomyopathy","volume":"76","author":"Sepp","year":"1996","journal-title":"Heart."},{"key":"2023072901474408500_Soteriouetal1993","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1242\/jcs.104.1.119","article-title":"A survey of interactions made by the giant protein titin","volume":"104","author":"Soteriou","year":"1993","journal-title":"J. Cell Sci."},{"key":"2023072901474408500_Sussmanetal1998","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1172\/JCI1167","article-title":"Myofibril degeneration caused by tropomodulin overexpression leads to dilated cardiomyopathy in juvenile mice","volume":"101","author":"Sussman","year":"1998","journal-title":"J. Clin. 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