{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T12:29:01Z","timestamp":1791289741928,"version":"4.3.0"},"reference-count":56,"publisher":"Rockefeller University Press","issue":"6","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2000,3,20]]},"abstract":"<jats:p>Kinesin superfamily proteins (KIFs) comprise several dozen molecular motor proteins. The KIF3 heterotrimer complex is one of the most abundantly and ubiquitously expressed KIFs in mammalian cells. To unveil the functions of KIF3, microinjection of function-blocking monovalent antibodies against KIF3 into cultured superior cervical ganglion (SCG) neurons was carried out. They significantly blocked fast axonal transport and brought about inhibition of neurite extension. A yeast two-hybrid binding assay revealed the association of fodrin with the KIF3 motor through KAP3. This was further confirmed by using vesicles collected from large bundles of axons (cauda equina), from which membranous vesicles could be prepared in pure preparations. Both immunoprecipitation and immunoelectron microscopy indicated the colocalization of fodrin and KIF3 on the same vesicles, the results reinforcing the evidence that the cargo of the KIF3 motor consists of fodrin-associating vesicles. In addition, pulse-labeling study implied partial comigration of both molecules as fast flow components. Taken together, the KIF3 motor is engaged in fast axonal transport that conveys membranous components important for neurite extension.<\/jats:p>","DOI":"10.1083\/jcb.148.6.1255","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T16:45:41Z","timestamp":1027701941000},"page":"1255-1266","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":184,"title":["Kinesin Superfamily Protein 3 (Kif3) Motor Transports Fodrin-Associating Vesicles Important for Neurite Building"],"prefix":"10.1083","volume":"148","author":[{"given":"Sen","family":"Takeda","sequence":"first","affiliation":[{"name":"aDepartment of Cell Biology and Anatomy, University of Tokyo, Graduate School of Medicine, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroto","family":"Yamazaki","sequence":"additional","affiliation":[{"name":"aDepartment of Cell Biology and Anatomy, University of Tokyo, Graduate School of Medicine, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dae-Hyun","family":"Seog","sequence":"additional","affiliation":[{"name":"aDepartment of Cell Biology and Anatomy, University of Tokyo, Graduate School of Medicine, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshimitsu","family":"Kanai","sequence":"additional","affiliation":[{"name":"aDepartment of Cell Biology and Anatomy, University of Tokyo, Graduate School of Medicine, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sumio","family":"Terada","sequence":"additional","affiliation":[{"name":"aDepartment of Cell Biology and Anatomy, University of Tokyo, Graduate School of Medicine, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nobutaka","family":"Hirokawa","sequence":"additional","affiliation":[{"name":"aDepartment of Cell Biology and Anatomy, University of Tokyo, Graduate School of Medicine, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2000,3,20]]},"reference":[{"key":"2023072902093517500_Aizawaetal1992","doi-asserted-by":"crossref","first-page":"1287","DOI":"10.1083\/jcb.119.5.1287","article-title":"Kinesin family in murine central nervous system","volume":"119","author":"Aizawa","year":"1992","journal-title":"J. Cell Biol."},{"key":"2023072902093517500_Ausubeletal1997"},{"key":"2023072902093517500_Bamburgetal1986","doi-asserted-by":"crossref","first-page":"788","DOI":"10.1038\/321788a0","article-title":"Assembly of microtubules at the tip of growing axons","volume":"321","author":"Bamburg","year":"1986","journal-title":"Nature."},{"key":"2023072902093517500_Beechetal1996","doi-asserted-by":"crossref","first-page":"889","DOI":"10.1242\/jcs.109.4.889","article-title":"Localization of kinesin superfamily proteins to the connecting cilium of fish photoreceptors","volume":"109","author":"Beech","year":"1996","journal-title":"J. Cell Sci."},{"key":"2023072902093517500_Brady1985","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1038\/317073a0","article-title":"A novel brain ATPase with properties expected for the fast axonal transport motor","volume":"317","author":"Brady","year":"1985","journal-title":"Nature."},{"key":"2023072902093517500_Bradyetal1990","doi-asserted-by":"crossref","first-page":"1061","DOI":"10.1073\/pnas.87.3.1061","article-title":"A monoclonal antibody against kinesin inhibits both anterograde and retrograde fast axonal transport in squid axoplasm","volume":"87","author":"Brady","year":"1990","journal-title":"Proc. Natl. Acad. Sci. USA."},{"key":"2023072902093517500_Cheneyetal1983","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1002\/cm.970030529","article-title":"Intracellular movement of fodrin","volume":"3","author":"Cheney","year":"1983","journal-title":"Cell Motility."},{"key":"2023072902093517500_Coleetal1993","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1038\/366268a0","article-title":"Novel heterotrimeric kinesin-related protein purified from sea urchin eggs","volume":"366","author":"Cole","year":"1993","journal-title":"Nature."},{"key":"2023072902093517500_Coleetal1998","doi-asserted-by":"crossref","first-page":"993","DOI":"10.1083\/jcb.141.4.993","article-title":"Kinesin-II\u2013dependent intraflagellar transport (IFT)IFT particles contain proteins required for ciliary assembly in Caenorhabditis elegans sensory neurons","volume":"141","author":"Cole","year":"1998","journal-title":"J. Cell Biol."},{"key":"2023072902093517500_DeCamillietal1983","doi-asserted-by":"crossref","first-page":"1355","DOI":"10.1083\/jcb.96.5.1355","article-title":"Synapsin I (protein I), a nerve terminal-specific phosphoprotein. II. Its specific association with synaptic vesicles demonstrated by immunocytochemistry in agarose-embedded synaptosomes","volume":"96","author":"De Camilli","year":"1983","journal-title":"J. Cell Biol."},{"key":"2023072902093517500_N0x1e75180N0x2ecfc40"},{"key":"2023072902093517500_Elluruetal1995","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1091\/mbc.6.1.21","article-title":"Fast axonal transport of kinesin in the rat visual systemfunctionality of kinesin heavy chain isoforms","volume":"6","author":"Elluru","year":"1995","journal-title":"Mol. Biol. Cell."},{"key":"2023072902093517500_Funakoshietal1996","doi-asserted-by":"crossref","first-page":"1347","DOI":"10.1083\/jcb.133.6.1347","article-title":"Active transport of photoactivated tubulin molecules in growing axons revealed by a new electron microscopic analysis","volume":"133","author":"Funakoshi","year":"1996","journal-title":"J. Cell Biol."},{"key":"2023072902093517500_GindhartandGoldstein1996","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1016\/S0962-8924(96)20037-6","article-title":"Armadillo repeats in the SpKAP115 subunit of kinesin-II","volume":"6","author":"Gindhart","year":"1996","journal-title":"Trends Cell Biol."},{"key":"2023072902093517500_Higginsetal1991","first-page":"177","article-title":"Tissue culture of mammalian autonomic neurons","volume-title":"Culturing Nerve Cells","author":"Higgins","year":"1991"},{"key":"2023072902093517500_Hirokawa1982","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1083\/jcb.94.1.129","article-title":"Cross-linker system between neurofilaments, microtubules, and membranous organelles in frog axons revealed by the quick-freeze, deep-etching method","volume":"94","author":"Hirokawa","year":"1982","journal-title":"J. Cell Biol."},{"key":"2023072902093517500_Hirokawa1996","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/0962-8924(96)10003-9","article-title":"Organelle transport along microtubulesthe role of KIFs","volume":"6","author":"Hirokawa","year":"1996","journal-title":"Trends Cell Biol."},{"key":"2023072902093517500_Hirokawa1998","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1126\/science.279.5350.519","article-title":"Kinesin and dynein superfamily proteins and the mechanism of organelle transport","volume":"279","author":"Hirokawa","year":"1998","journal-title":"Science."},{"key":"2023072902093517500_Hirokawaetal1983","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1016\/0092-8674(83)90080-6","article-title":"Location of a protein of the fodrin-spectrin-TW260\/240 family in the mouse intestinal brush border","volume":"32","author":"Hirokawa","year":"1983","journal-title":"Cell."},{"key":"2023072902093517500_Hirokawaetal1990","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1083\/jcb.111.3.1027","article-title":"Brain dynein (MAP1C) localizes on both anterogradely and retrogradely transported membranous organelles in vivo","volume":"111","author":"Hirokawa","year":"1990","journal-title":"J. Cell Biol."},{"key":"2023072902093517500_Holleranetal1996","doi-asserted-by":"crossref","first-page":"1815","DOI":"10.1083\/jcb.135.6.1815","article-title":"Centractin (ARP1) associates with spectrin revealing a potential mechanism to link dynactin to intracellular organelles","volume":"135","author":"Holleran","year":"1996","journal-title":"J. Cell Biol."},{"key":"2023072902093517500_Kondoetal1994","doi-asserted-by":"crossref","first-page":"1095","DOI":"10.1083\/jcb.125.5.1095","article-title":"KIF3A is a new microtubule-based anterograde motor in the nerve axon","volume":"125","author":"Kondo","year":"1994","journal-title":"J. Cell Biol."},{"key":"2023072902093517500_Kozminskietal1995","doi-asserted-by":"crossref","first-page":"1517","DOI":"10.1083\/jcb.131.6.1517","article-title":"The kinesin-like protein FLA10 is involved in motility associated with the flagellar membrane","volume":"131","author":"Kozminski","year":"1995","journal-title":"J. Cell Biol."},{"key":"2023072902093517500_LeBotetal1998","doi-asserted-by":"crossref","first-page":"1559","DOI":"10.1083\/jcb.143.6.1559","article-title":"Role of xklp3, a subunit of the Xenopus kinesin II heterotrimeric complex, in membrane transport between the endoplasmic reticulum and the Golgi apparatus","volume":"143","author":"Le Bot","year":"1998","journal-title":"J. Cell Biol."},{"key":"2023072902093517500_Leeetal1993","doi-asserted-by":"crossref","first-page":"1797","DOI":"10.1083\/jcb.123.6.1797","article-title":"Cell shape and interaction defects in alpha-spectrin mutants of Drosophila melanogaster","volume":"123","author":"Lee","year":"1993","journal-title":"J. Cell Biol."},{"key":"2023072902093517500_LevineandWillard1981","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1083\/jcb.90.3.631","article-title":"Fodrinaxonally transported polypeptides associated with the internal periphery of many cells","volume":"90","author":"Levine","year":"1981","journal-title":"J. Cell Biol."},{"key":"2023072902093517500_Marszaleketal1999","doi-asserted-by":"crossref","first-page":"5043","DOI":"10.1073\/pnas.96.9.5043","article-title":"Situs inversus and embryonic ciliary morphogenesis defects in mouse mutants lacking the KIF3A subunit of kinesin-II","volume":"96","author":"Marszalek","year":"1999","journal-title":"Proc. Natl. Acad. Sci. USA."},{"key":"2023072902093517500_Martensonetal1993","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1038\/366066a0","article-title":"Fast axonal transport is required for growth cone advance","volume":"366","author":"Martenson","year":"1993","journal-title":"Nature."},{"key":"2023072902093517500_Martinetal1999","doi-asserted-by":"crossref","first-page":"3717","DOI":"10.1091\/mbc.10.11.3717","article-title":"Cytoplasmic dynein, the dynactin complex, and kinesin are interdependent and essential for fast axonal transport","volume":"10","author":"Martin","year":"1999","journal-title":"Mol. Biol. Cell."},{"key":"2023072902093517500_Masliahetal1991","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0304-3940(91)90707-Z","article-title":"Abnormal brain spectrin immunoreactivity in sprouting neurons in Alzheimer disease","volume":"129","author":"Masliah","year":"1991","journal-title":"Neurosci. Lett."},{"key":"2023072902093517500_MorrisandScholey1997","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.1083\/jcb.138.5.1009","article-title":"Heterotrimeric kinesin-II is required for the assembly of motile 9+2 ciliary axonemes on sea urchin embryos","volume":"138","author":"Morris","year":"1997","journal-title":"J. Cell Biol."},{"key":"2023072902093517500_Muresanetal1997","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1006\/exer.1996.0261","article-title":"Evidence for kinesin-related proteins associated with the axoneme of retinal photoreceptors","volume":"64","author":"Muresan","year":"1997","journal-title":"Exp. Eye Res."},{"key":"2023072902093517500_Muresanetal1998","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1091\/mbc.9.3.637","article-title":"KIF3C and KIF3A form a novel neuronal heteromeric kinesin that associates with membrane vesicles","volume":"9","author":"Muresan","year":"1998","journal-title":"Mol. Biol. Cell."},{"key":"2023072902093517500_Nakajimaetal1997","doi-asserted-by":"crossref","first-page":"917","DOI":"10.2108\/zsj.14.917","article-title":"Molecular cloning and expression of the KIF3A gene in the frog brain and testis","volume":"14","author":"Nakajima","year":"1997","journal-title":"Zool. Sci."},{"key":"2023072902093517500_Nishiyeetal1988","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1016\/0168-0102(88)90044-2","article-title":"A new method for electron microscopic observation of isolated synaptic vesicles labelled with monoclonal antibody","volume":"5","author":"Nishiye","year":"1988","journal-title":"Neurosci. Res."},{"key":"2023072902093517500_Nonakaetal1998","doi-asserted-by":"crossref","first-page":"829","DOI":"10.1016\/S0092-8674(00)81705-5","article-title":"Randomization of left-right asymmetry due to loss of nodal cilia generating leftward flow of extraembryonic fluid in mice lacking KIF3B motor protein","volume":"95","author":"Nonaka","year":"1998","journal-title":"Cell."},{"key":"2023072902093517500_OkabeandHirokawa1988","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1083\/jcb.107.2.651","article-title":"Microtubule dynamics in nerve cellsanalysis using microinjection of biotinylated tubulin into PC12 cells","volume":"107","author":"Okabe","year":"1988","journal-title":"J. Cell Biol."},{"key":"2023072902093517500_Okadaetal1995a","doi-asserted-by":"crossref","first-page":"3053","DOI":"10.1523\/JNEUROSCI.15-04-03053.1995","article-title":"The activation of protein kinase A pathway selectively inhibits anterograde axonal transport of vesicles but not mitochondria transport or retrograde transport in vivo","volume":"15","author":"Okada","year":"1995","journal-title":"J. Neurosci."},{"key":"2023072902093517500_Okadaetal1995b","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1016\/0092-8674(95)90538-3","article-title":"The neuron-specific kinesin superfamily protein KIF1A is a unique monomeric motor for anterograde axonal transport of synaptic vesicle precursors","volume":"81","author":"Okada","year":"1995","journal-title":"Cell."},{"key":"2023072902093517500_Pesaventoetal1994","doi-asserted-by":"crossref","first-page":"1041","DOI":"10.1083\/jcb.127.4.1041","article-title":"Characterization of the KLP68D kinesin-like protein in Drosophilapossible roles in axonal transport","volume":"127","author":"Pesavento","year":"1994","journal-title":"J. Cell Biol."},{"key":"2023072902093517500_Rayetal1999","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1083\/jcb.147.3.507","article-title":"Kinesin-II is required for axonal transport of choline acetyltransferase in Drosophila","volume":"147","author":"Ray","year":"1999","journal-title":"J. Cell Biol."},{"key":"2023072902093517500_Rosenbaumetal1999","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1083\/jcb.144.3.385","article-title":"Intraflagellar transportthe eyes have it","volume":"144","author":"Rosenbaum","year":"1999","journal-title":"J. Cell Biol."},{"key":"2023072902093517500_Shimizuetal1996","doi-asserted-by":"crossref","first-page":"27013","DOI":"10.1074\/jbc.271.43.27013","article-title":"SMAP, an Smg GDS-associated protein having arm repeats and phosphorylated by src tyrosine kinase","volume":"271","author":"Shimizu","year":"1996","journal-title":"J. Biol. Chem."},{"key":"2023072902093517500_Signoretal1999","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1091\/mbc.10.2.345","article-title":"Two heteromeric kinesin complexes in chemosensory neurons and sensory cilia of Caenorhabditis elegans","volume":"10","author":"Signor","year":"1999","journal-title":"Mol. Biol. Cell."},{"key":"2023072902093517500_SobueandKanda1989","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1016\/0896-6273(89)90255-9","article-title":"Alpha-actinins, calspectin (brain spectrin or fodrin), and actin participate in adhesion and movement of growth cones","volume":"3","author":"Sobue","year":"1989","journal-title":"Neuron."},{"key":"2023072902093517500_Takedaetal1994","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1083\/jcb.127.1.173","article-title":"Differential dynamics of neurofilament-H protein and neurofilament-L protein in neuron","volume":"127","author":"Takeda","year":"1994","journal-title":"J. Cell Biol."},{"key":"2023072902093517500_Takedaetal1995","doi-asserted-by":"crossref","first-page":"1257","DOI":"10.1016\/0896-6273(95)90272-4","article-title":"Tubulin dynamics in neuronal axons of zebrafish embryos","volume":"14","author":"Takeda","year":"1995","journal-title":"Neuron."},{"key":"2023072902093517500_Takedaetal1999","doi-asserted-by":"crossref","first-page":"825","DOI":"10.1083\/jcb.145.4.825","article-title":"Left-right asymmetry and kinesin superfamily protein KIF3Anew insights in determination of laterality and mesoderm induction by kif3A\u2212\/\u2212 mice analysis","volume":"145","author":"Takeda","year":"1999","journal-title":"J. Cell Biol."},{"key":"2023072902093517500_Takenakaetal1992","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1016\/0006-8993(92)91577-2","article-title":"Effect of neurotransmitters on axoplasmic transportacetylcholine effect on superior cervical ganglion cells","volume":"588","author":"Takenaka","year":"1992","journal-title":"Brain Res."},{"key":"2023072902093517500_Tumaetal1998","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1083\/jcb.143.6.1547","article-title":"Heterotrimeric kinesin II is the microtubule motor protein responsible for pigment dispersion in Xenopus melanophores","volume":"143","author":"Tuma","year":"1998","journal-title":"J. Cell Biol."},{"key":"2023072902093517500_Valeetal1985","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/S0092-8674(85)80099-4","article-title":"Identification of a novel force-generating protein, kinesin, involved in microtubule-based motility","volume":"42","author":"Vale","year":"1985","journal-title":"Cell."},{"key":"2023072902093517500_Waltheretal1994","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1083\/jcb.126.1.175","article-title":"The FLA10 gene encodes a novel kinesin-homologous protein","volume":"126","author":"Walther","year":"1994","journal-title":"J. Cell Biol."},{"key":"2023072902093517500_Waterman-Storeretal1997","doi-asserted-by":"crossref","first-page":"12180","DOI":"10.1073\/pnas.94.22.12180","article-title":"The interaction between cytoplasmic dynein and dynactin is required for fast axonal transport","volume":"94","author":"Waterman-Storer","year":"1997","journal-title":"Proc. Natl. Acad. Sci. USA."},{"key":"2023072902093517500_Yamazakietal1995","doi-asserted-by":"crossref","first-page":"1387","DOI":"10.1083\/jcb.130.6.1387","article-title":"KIF3A\/Ba heterodimeric kinesin superfamily protein that works as a microtubule plus end-directed motor for membrane organelle transport","volume":"130","author":"Yamazaki","year":"1995","journal-title":"J. Cell Biol."},{"key":"2023072902093517500_Yamazakietal1996","doi-asserted-by":"crossref","first-page":"8443","DOI":"10.1073\/pnas.93.16.8443","article-title":"Cloning and characterization of KAP3a novel kinesin superfamily-associated protein of KIF3A\/3B","volume":"93","author":"Yamazaki","year":"1996","journal-title":"Proc. Natl. Acad. Sci. USA."},{"key":"2023072902093517500_YangandGoldstein1998","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1091\/mbc.9.2.249","article-title":"Characterization of the KIF3C neural kinesin-like motor from mouse","volume":"9","author":"Yang","year":"1998","journal-title":"Mol. Biol. Cell."}],"container-title":["The Journal of Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/148\/6\/1255\/1857951\/9905010.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/148\/6\/1255\/1857951\/9905010.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,29]],"date-time":"2023-07-29T02:10:26Z","timestamp":1690596626000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jcb\/article\/148\/6\/1255\/45008\/Kinesin-Superfamily-Protein-3-Kif3-Motor"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,3,20]]},"references-count":56,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2000,3,20]]}},"URL":"https:\/\/doi.org\/10.1083\/jcb.148.6.1255","relation":{},"ISSN":["0021-9525","1540-8140"],"issn-type":[{"value":"0021-9525","type":"print"},{"value":"1540-8140","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,3,20]]}}}