{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T20:50:23Z","timestamp":1791406223832,"version":"4.3.3"},"reference-count":60,"publisher":"Rockefeller University Press","issue":"1","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2000,4,3]]},"abstract":"<jats:p>Plakins are cytoskeletal linker proteins initially thought to interact exclusively with intermediate filaments (IFs), but recently were found to associate additionally with actin and microtubule networks. Here, we report on ACF7, a mammalian orthologue of the Drosophila kakapo plakin genetically involved in epidermal\u2013muscle adhesion and neuromuscular junctions. While ACF7\/kakapo is divergent from other plakins in its IF-binding domain, it has at least one actin (Kd = 0.35 \u03bcM) and one microtubule (Kd \u223c6 \u03bcM) binding domain. Similar to its fly counterpart, ACF7 is expressed in the epidermis. In well spread epidermal keratinocytes, ACF7 discontinuously decorates the cytoskeleton at the cell periphery, including microtubules (MTs) and actin filaments (AFs) that are aligned in parallel converging at focal contacts. Upon calcium induction of intercellular adhesion, ACF7 and the cytoskeleton reorganize at cell\u2013cell borders but with different kinetics from adherens junctions and desmosomes. Treatments with cytoskeletal depolymerizing drugs reveal that ACF7's cytoskeletal association is dependent upon the microtubule network, but ACF7 also appears to stabilize actin at sites where microtubules and microfilaments meet. We posit that ACF7 may function in microtubule dynamics to facilitate actin\u2013microtubule interactions at the cell periphery and to couple the microtubule network to cellular junctions. These attributes provide a clear explanation for the kakapo mutant phenotype in flies.<\/jats:p>","DOI":"10.1083\/jcb.149.1.195","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T16:45:41Z","timestamp":1027701941000},"page":"195-208","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":149,"title":["An Epidermal Plakin That Integrates Actin and Microtubule Networks at Cellular Junctions"],"prefix":"10.1083","volume":"149","author":[{"given":"Iakowos","family":"Karakesisoglou","sequence":"first","affiliation":[{"name":"aHoward Hughes Medical Institute, Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, Illinois 60637"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanmin","family":"Yang","sequence":"additional","affiliation":[{"name":"aHoward Hughes Medical Institute, Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, Illinois 60637"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elaine","family":"Fuchs","sequence":"additional","affiliation":[{"name":"aHoward Hughes Medical Institute, Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, Illinois 60637"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2000,4,3]]},"reference":[{"key":"2023072902085957400_Andraetal1997","doi-asserted-by":"crossref","first-page":"3143","DOI":"10.1101\/gad.11.23.3143","article-title":"Targeted inactivation of plectin reveals essential function in maintaining the integrity of skin, muscle, and heart cytoarchitecture","volume":"11","author":"Andra","year":"1997","journal-title":"Genes Dev."},{"key":"2023072902085957400_Andraetal1998","doi-asserted-by":"crossref","first-page":"3442","DOI":"10.1101\/gad.12.21.3442","article-title":"Not just scaffoldingplectin regulates actin dynamics in cultured cells","volume":"12","author":"Andra","year":"1998","journal-title":"Genes Dev"},{"key":"2023072902085957400_Bernieretal1996","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1006\/geno.1996.0587","article-title":"Cloning and characterization of mouse ACF7, a novel member of the dystonin subfamily of actin binding proteins","volume":"39","author":"Bernier","year":"1996","journal-title":"Genomics"},{"key":"2023072902085957400_Bershadskyetal1978","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1016\/0309-1651(78)90020-6","article-title":"Microtubular system in cultured mouse epithelial cells","volume":"2","author":"Bershadsky","year":"1978","journal-title":"Cell Biol. 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