{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T12:06:22Z","timestamp":1791201982276,"version":"4.2.0"},"reference-count":33,"publisher":"Rockefeller University Press","issue":"1","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2000,7,10]]},"abstract":"<jats:p>Fibroblast growth factor 2 (FGF-2) is a powerful mitogen involved in proliferation, differentiation, and survival of various cells including neurons. FGF-2 expression is translationally regulated; in particular, the FGF-2 mRNA contains an internal ribosome entry site (IRES) allowing cap-independent translation. Here, we have analyzed FGF-2 IRES tissue specificity ex vivo and in vivo by using a dual luciferase bicistronic vector. This IRES was active in most transiently transfected human and nonhuman cell types, with a higher activity in p53 \u2212\/\u2212 osteosarcoma and neuroblastoma cell lines. Transgenic mice were generated using bicistronic transgenes with FGF-2 IRES or encephalomyocarditis virus (EMCV) IRES. Measurements of luciferase activity revealed high FGF-2 IRES activity in 11-d-old embryos (E11) but not in the placenta; activity was high in the heart and brain of E16. FGF-2 IRES activity was low in most organs of the adult, but exceptionally high in the brain. Such spatiotemporal variations were not observed with the EMCV IRES. These data, demonstrating the strong tissue specificity of a mammalian IRES in vivo, suggest a pivotal role of translational IRES- dependent activation of FGF-2 expression during embryogenesis and in adult brain. FGF-2 IRES could constitute, thus, a powerful tool for gene transfer in the central nervous system.<\/jats:p>","DOI":"10.1083\/jcb.150.1.275","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T16:45:41Z","timestamp":1027701941000},"page":"275-281","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":129,"title":["Fibroblast Growth Factor 2 Internal Ribosome Entry Site (Ires) Activity Ex Vivo and in Transgenic Mice Reveals a Stringent Tissue-Specific Regulation"],"prefix":"10.1083","volume":"150","author":[{"given":"Laurent","family":"Cr\u00e9ancier","sequence":"first","affiliation":[{"name":"aInstitut National de la Sant\u00e9 et de la Recherche M\u00e9dicale U397, Endocrinologie et Communication Cellulaire, Institut F\u00e9d\u00e9ratif de Recherche Louis Bugnard, C.H.U. Rangueil, 31403 Toulouse Cedex 04, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominique","family":"Morello","sequence":"additional","affiliation":[{"name":"bCentre de Biologie du D\u00e9veloppement, UMR 5547, Universit\u00e9 Paul Sabatier, B\u00e2timent 4R3, 118 Route de Narbonne, 31062 Toulouse Cedex 04, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pascale","family":"Mercier","sequence":"additional","affiliation":[{"name":"cInstitut de Pharmacologie et Biologie Structurale, UPR 9062, 31077 Toulouse Cedex 04, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne-Catherine","family":"Prats","sequence":"additional","affiliation":[{"name":"aInstitut National de la Sant\u00e9 et de la Recherche M\u00e9dicale U397, Endocrinologie et Communication Cellulaire, Institut F\u00e9d\u00e9ratif de Recherche Louis Bugnard, C.H.U. 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