{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T16:28:08Z","timestamp":1791390488007,"version":"4.3.3"},"reference-count":56,"publisher":"Rockefeller University Press","issue":"3","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2000,8,7]]},"abstract":"<jats:p>The Listeria monocytogenes ActA protein induces actin-based motility by enhancing the actin nucleating activity of the host Arp2\/3 complex. Using systematic truncation analysis, we identified a 136-residue NH2-terminal fragment that was fully active in stimulating nucleation in vitro. Further deletion analysis demonstrated that this fragment contains three regions, which are important for nucleation and share functional and\/or limited sequence similarity with host WASP family proteins: an acidic stretch, an actin monomer\u2013binding region, and a cofilin homology sequence. To determine the contribution of each region to actin-based motility, we compared the biochemical activities of ActA derivatives with the phenotypes of corresponding mutant bacteria in cells. The acidic stretch functions to increase the efficiency of actin nucleation, the rate and frequency of motility, and the effectiveness of cell\u2013cell spread. The monomer-binding region is required for actin nucleation in vitro, but not for actin polymerization or motility in infected cells, suggesting that redundant mechanisms may exist to recruit monomer in host cytosol. The cofilin homology sequence is critical for stimulating actin nucleation with the Arp2\/3 complex in vitro, and is essential for actin polymerization and motility in cells. These data demonstrate that each region contributes to actin-based motility, and that the cofilin homology sequence plays a principal role in activation of the Arp2\/3 complex, and is an essential determinant of L. monocytogenes pathogenesis.<\/jats:p>","DOI":"10.1083\/jcb.150.3.527","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T16:45:41Z","timestamp":1027701941000},"page":"527-538","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":172,"title":["Three Regions within Acta Promote Arp2\/3 Complex-Mediated Actin Nucleation and <i>Listeria monocytogenes<\/i> Motility"],"prefix":"10.1083","volume":"150","author":[{"given":"Justin","family":"Skoble","sequence":"first","affiliation":[{"name":"aDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California 94720-3200"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel A.","family":"Portnoy","sequence":"additional","affiliation":[{"name":"aDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California 94720-3200"},{"name":"bSchool of Public Health, University of California, Berkeley, Berkeley, California 94720-3200"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew D.","family":"Welch","sequence":"additional","affiliation":[{"name":"aDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California 94720-3200"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2000,8,7]]},"reference":[{"key":"2023072902284241600_Banzaietal2000","doi-asserted-by":"crossref","first-page":"11987","DOI":"10.1074\/jbc.275.16.11987","article-title":"Essential role of neural Wiskott-Aldrich syndrome protein in neurite extension in PC12 cells and rat hippocampal primary culture cells","volume":"275","author":"Banzai","year":"2000","journal-title":"J. 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