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Filaments nucleated by Arp2\/3 complex and WASp in the presence of PIP2 and Cdc42 concentrated around lipid micelles and vesicles, providing that Cdc42 was GTP-bound and prenylated. Thus, the high concentration of WASp in neutrophils (9 \u03bcM) is dependent on interactions with both acidic lipids and GTP-Cdc42 to activate actin nucleation by Arp2\/3 complex. The results also suggest that membrane binding increases the local concentrations of Cdc42 and WASp, favoring their interaction.<\/jats:p>","DOI":"10.1083\/jcb.150.6.1311","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T16:45:41Z","timestamp":1027701941000},"page":"1311-1320","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":446,"title":["Activation by Cdc42 and Pip2 of Wiskott-Aldrich Syndrome Protein (Wasp) Stimulates Actin Nucleation by Arp2\/3 Complex"],"prefix":"10.1083","volume":"150","author":[{"given":"Henry N.","family":"Higgs","sequence":"first","affiliation":[{"name":"aThe Salk Institute for Biological Studies, La Jolla, California 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas D.","family":"Pollard","sequence":"additional","affiliation":[{"name":"aThe Salk Institute for Biological Studies, La Jolla, California 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2000,9,18]]},"reference":[{"key":"2023072902374393300_Abdul-Mananetal1999","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1038\/20726","article-title":"Structure of Cdc42 in complex with the GTPase-binding domain of the \u2018Wiskott-Aldrich Syndrome\u2019 protein","volume":"399","author":"Abdul-Manan","year":"1999","journal-title":"Nature"},{"key":"2023072902374393300_Baillyetal1999","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1083\/jcb.145.2.331","article-title":"Relationship between Arp2\/3 complex and the barbed ends of actin filaments at the leading edge of carcinoma cells after epidermal growth factor stimulation","volume":"145","author":"Bailly","year":"1999","journal-title":"J. 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