{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T08:21:42Z","timestamp":1792570902069,"version":"4.5.0"},"reference-count":44,"publisher":"Rockefeller University Press","issue":"6","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2000,9,18]]},"abstract":"<jats:p>Although the crucial role of Ca2+ influx in lymphocyte activation has been well documented, little is known about the properties or expression levels of Ca2+ channels in normal human T lymphocytes. The use of Na+ as the permeant ion in divalent-free solution permitted Ca2+ release-activated Ca2+ (CRAC) channel activation, kinetic properties, and functional expression levels to be investigated with single channel resolution in resting and phytohemagglutinin (PHA)-activated human T cells. Passive Ca2+ store depletion resulted in the opening of 41-pS CRAC channels characterized by high open probabilities, voltage-dependent block by extracellular Ca2+ in the micromolar range, selective Ca2+ permeation in the millimolar range, and inactivation that depended upon intracellular Mg2+ ions. The number of CRAC channels per cell increased greatly from \u223c15 in resting T cells to \u223c140 in activated T cells. Treatment with the phorbol ester PMA also increased CRAC channel expression to \u223c60 channels per cell, whereas the immunosuppressive drug cyclosporin A (1 \u03bcM) suppressed the PHA-induced increase in functional channel expression. Capacitative Ca2+ influx induced by thapsigargin was also significantly enhanced in activated T cells. We conclude that a surprisingly low number of CRAC channels are sufficient to mediate Ca2+ influx in human resting T cells, and that the expression of CRAC channels increases \u223c10-fold during activation, resulting in enhanced Ca2+ signaling.<\/jats:p>","DOI":"10.1083\/jcb.150.6.1435","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T16:46:23Z","timestamp":1027701983000},"page":"1435-1444","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":71,"title":["Single Channel Properties and Regulated Expression of Ca2+ Release-Activated Ca2+ (Crac) Channels in Human T Cells"],"prefix":"10.1083","volume":"150","author":[{"given":"Alla F.","family":"Fomina","sequence":"first","affiliation":[{"name":"aDepartment of Physiology and Biophysics, University of California Irvine, Irvine, California 92697-4561"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher M.","family":"Fanger","sequence":"additional","affiliation":[{"name":"aDepartment of Physiology and Biophysics, University of California Irvine, Irvine, California 92697-4561"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. Ashot","family":"Kozak","sequence":"additional","affiliation":[{"name":"aDepartment of Physiology and Biophysics, University of California Irvine, Irvine, California 92697-4561"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael D.","family":"Cahalan","sequence":"additional","affiliation":[{"name":"aDepartment of Physiology and Biophysics, University of California Irvine, Irvine, California 92697-4561"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2000,9,18]]},"reference":[{"key":"2023072902291250400_Byrneetal1988","doi-asserted-by":"crossref","first-page":"3249","DOI":"10.4049\/jimmunol.141.10.3249","article-title":"Differential activation requirements for virgin and memory T cells","volume":"141","author":"Byrne","year":"1988","journal-title":"J. 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