{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T13:58:05Z","timestamp":1791122285764,"version":"4.1.0"},"reference-count":50,"publisher":"Rockefeller University Press","issue":"2","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2000,10,16]]},"abstract":"<jats:p>Cell migration represents an important cellular response that utilizes cytoskeletal reorganization as its driving force. Here, we describe a new signaling cascade linking PDGF receptor stimulation to actin rearrangements and cell migration. We demonstrate that PDGF activates Cdc42 and its downstream effector N-WASP to mediate filopodia formation, actin stress fiber disassembly, and a reduction in focal adhesion complexes. Induction of the Cdc42 pathway is independent of phosphoinositide 3-kinase (PI3K) enzymatic activity, but it is dependent on the p85\u03b1 regulatory subunit of PI3K. Finally, data are provided showing that activation of this pathway is required for PDGF-induced cell migration on collagen. These observations show the essential role of the PI3K regulatory subunit p85\u03b1 in controlling PDGF receptor\u2013induced cytoskeletal changes and cell migration, illustrating a novel signaling pathway that links receptor stimulation at the cell membrane with actin dynamics.<\/jats:p>","DOI":"10.1083\/jcb.151.2.249","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T16:45:41Z","timestamp":1027701941000},"page":"249-262","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":217,"title":["Role of the Pi3k Regulatory Subunit in the Control of Actin Organization and Cell Migration"],"prefix":"10.1083","volume":"151","author":[{"given":"Concepci\u00f3n","family":"Jim\u00e9nez","sequence":"first","affiliation":[{"name":"aDepartment of Immunology and Oncology, Centro Nacional de Biotecnolog\u00eda,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosario Armas","family":"Portela","sequence":"additional","affiliation":[{"name":"bCentro de Biolog\u00eda Molecular, Consejo Superior de Investigaciones Cient\u00edficas, Universidad Aut\u00f3noma de Madrid, Cantoblanco, Madrid E-28049, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"Mellado","sequence":"additional","affiliation":[{"name":"aDepartment of Immunology and Oncology, Centro Nacional de Biotecnolog\u00eda,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose Miguel","family":"Rodr\u00edguez-Frade","sequence":"additional","affiliation":[{"name":"aDepartment of Immunology and Oncology, Centro Nacional de Biotecnolog\u00eda,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Collard","sequence":"additional","affiliation":[{"name":"cThe Netherlands Cancer Institute, Division of Cell Biology, 1066 CX, Amsterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Serrano","sequence":"additional","affiliation":[{"name":"aDepartment of Immunology and Oncology, Centro Nacional de Biotecnolog\u00eda,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Mart\u00ednez-A","sequence":"additional","affiliation":[{"name":"aDepartment of Immunology and Oncology, Centro Nacional de Biotecnolog\u00eda,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jesus","family":"Avila","sequence":"additional","affiliation":[{"name":"bCentro de Biolog\u00eda Molecular, Consejo Superior de Investigaciones Cient\u00edficas, Universidad Aut\u00f3noma de Madrid, Cantoblanco, Madrid E-28049, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana C.","family":"Carrera","sequence":"additional","affiliation":[{"name":"aDepartment of Immunology and Oncology, Centro Nacional de Biotecnolog\u00eda,"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2000,10,16]]},"reference":[{"key":"2023072902350096100_Aspenstrom1999","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/S0955-0674(99)80011-8","article-title":"Effectors for the Rho GTPases","volume":"11","author":"Aspenstrom","year":"1999","journal-title":"Curr. 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