{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T05:33:26Z","timestamp":1791092006598,"version":"4.1.0"},"reference-count":39,"publisher":"Rockefeller University Press","issue":"3","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2001,2,5]]},"abstract":"<jats:p>We identified a novel adaptor protein that contains a Src homology (SH)3 domain, SH3 binding proline-rich sequences, and a leucine zipper-like motif and termed this protein WASP interacting SH3 protein (WISH). WISH is expressed predominantly in neural tissues and testis. It bound Ash\/Grb2 through its proline-rich regions and neural Wiskott-Aldrich syndrome protein (N-WASP) through its SH3 domain. WISH strongly enhanced N-WASP\u2013induced Arp2\/3 complex activation independent of Cdc42 in vitro, resulting in rapid actin polymerization. Furthermore, coexpression of WISH and N-WASP induced marked formation of microspikes in Cos7 cells, even in the absence of stimuli. An N-WASP mutant (H208D) that cannot bind Cdc42 still induced microspike formation when coexpressed with WISH. We also examined the contribution of WISH to a rapid actin polymerization induced by brain extract in vitro. Arp2\/3 complex was essential for brain extract\u2013induced rapid actin polymerization. Addition of WISH to extracts increased actin polymerization as Cdc42 did. However, WISH unexpectedly could activate actin polymerization even in N-WASP\u2013depleted extracts. These findings suggest that WISH activates Arp2\/3 complex through N-WASP\u2013dependent and \u2013independent pathways without Cdc42, resulting in the rapid actin polymerization required for microspike formation.<\/jats:p>","DOI":"10.1083\/jcb.152.3.471","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T16:46:23Z","timestamp":1027701983000},"page":"471-482","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":131,"title":["A Novel Neural Wiskott-Aldrich Syndrome Protein (N-Wasp) Binding Protein, Wish, Induces Arp2\/3 Complex Activation Independent of Cdc42"],"prefix":"10.1083","volume":"152","author":[{"given":"Maiko","family":"Fukuoka","sequence":"first","affiliation":[{"name":"aDepartment of Biochemistry, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan"},{"name":"bDepartment of Biology, Faculty of Science, Chiba University, Chiba 263-8522, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiro","family":"Suetsugu","sequence":"additional","affiliation":[{"name":"aDepartment of Biochemistry, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroaki","family":"Miki","sequence":"additional","affiliation":[{"name":"aDepartment of Biochemistry, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kiyoko","family":"Fukami","sequence":"additional","affiliation":[{"name":"aDepartment of Biochemistry, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeshi","family":"Endo","sequence":"additional","affiliation":[{"name":"bDepartment of Biology, Faculty of Science, Chiba University, Chiba 263-8522, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tadaomi","family":"Takenawa","sequence":"additional","affiliation":[{"name":"aDepartment of Biochemistry, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2001,1,29]]},"reference":[{"key":"2023072211262878500_Arvidssonetal1994","first-page":"6715","article-title":"Tyr-716 in the platelet-derived growth factor beta-receptor kinase insert is involved in GRB2 binding and Ras activation","volume":"10","author":"Arvidsson","year":"1994","journal-title":"Mol. 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