{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T16:01:59Z","timestamp":1778688119624,"version":"3.51.4"},"reference-count":37,"publisher":"Rockefeller University Press","issue":"5","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2001,3,5]]},"abstract":"<jats:p>Epimorphin was recently described as a mesenchymal factor modulating morphogenesis of murine mammary ducts, skin, liver, and lung in vitro. In this study epimorphin was analyzed in a human, pancreatic adenocarcinoma cell line (A818-6) which develops single layer epithelial hollow spheres resembling normal pancreatic ductal structures in vitro. Soluble 34- and 31-kD isoforms of epimorphin were found in the culture supernatant of A818-6 cells. In lysates of A818-6 cells we detected the 34-and 31-kD isoforms and the dimers, and in lysates of fibroblasts the 150-kD tetramers of epimorphin additionally. A neutralizing monoclonal antibody against epimorphin (MC-1) efficiently blocked the development of hollow sphere structures from A818-6 cells. Coculture of A818-6 cells with fibroblasts stimulated the development of hollow sphere structures in general and increased differentiation in 5\u20136-d-old hollow spheres. A818-6 hollow sphere development in the presence of fibroblasts was also blocked by MC-1. In this novel system for human duct-like differentiation of pancreatic epithelial cells, we provide evidence for an autocrine and paracrine function of epimorphin as a major mediator for morphogenesis.<\/jats:p>","DOI":"10.1083\/jcb.152.5.911","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T16:46:23Z","timestamp":1027701983000},"page":"911-922","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":42,"title":["Autocrine Stimulation of Human Pancreatic Duct\u2013Like Development by Soluble Isoforms of Epimorphin in Vitro"],"prefix":"10.1083","volume":"152","author":[{"given":"Lasse","family":"Lehnert","sequence":"first","affiliation":[{"name":"aMolecular Oncology, Department of General and Thoracic Surgery"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Markus M.","family":"Lerch","sequence":"additional","affiliation":[{"name":"cDepartment of Medicine B, Westf\u00e4lische Wilhelms University, M\u00fcnster 48149, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yohei","family":"Hirai","sequence":"additional","affiliation":[{"name":"dSumitomo Industries, Yokohama 244-8588, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie-Luise","family":"Kruse","sequence":"additional","affiliation":[{"name":"bFirst Department of Medicine, Laboratory for Molecular Gastroenterology and Hepatology, Christian-Albrechts University, Kiel 24105, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wolff","family":"Schmiegel","sequence":"additional","affiliation":[{"name":"eMedical Clinic, Knappschaftskrankenhaus, Ruhr, University of Bochum, Bochum 44780, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Holger","family":"Kalthoff","sequence":"additional","affiliation":[{"name":"aMolecular Oncology, Department of General and Thoracic Surgery"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[2001,2,26]]},"reference":[{"key":"2023072211373097300_Akiyamaetal1999","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1046\/j.1365-2133.1999.03037.x","article-title":"Epimorphin expression during human foetal hair follicle development","volume":"141","author":"Akiyama","year":"1999","journal-title":"Br. 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