{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T15:43:42Z","timestamp":1792079022994,"version":"4.4.0"},"reference-count":52,"publisher":"Rockefeller University Press","issue":"6","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2002,3,18]]},"abstract":"<jats:p>The tight junction (TJ) and its adhesion molecules, claudins, are responsible for the barrier function of simple epithelia, but TJs have not been thought to play an important role in the barrier function of mammalian stratified epithelia, including the epidermis. Here we generated claudin-1\u2013deficient mice and found that the animals died within 1 d of birth with wrinkled skin. Dehydration assay and transepidermal water loss measurements revealed that in these mice the epidermal barrier was severely affected, although the layered organization of keratinocytes appeared to be normal. These unexpected findings prompted us to reexamine TJs in the epidermis of wild-type mice. Close inspection by immunofluorescence microscopy with an antioccludin monoclonal antibody, a TJ-specific marker, identified continuous TJs in the stratum granulosum, where claudin-1 and -4 were concentrated. The occurrence of TJs was also confirmed by ultrathin section EM. In claudin-1\u2013deficient mice, claudin-1 appeared to have simply been removed from these TJs, leaving occludin-positive (and also claudin-4\u2013positive) TJs. Interestingly, in the wild-type epidermis these occludin-positive TJs efficiently prevented the diffusion of subcutaneously injected tracer (\u223c600 D) toward the skin surface, whereas in the claudin-1\u2013deficient epidermis the tracer appeared to pass through these TJs. These findings provide the first evidence that continuous claudin-based TJs occur in the epidermis and that these TJs are crucial for the barrier function of the mammalian skin.<\/jats:p>","DOI":"10.1083\/jcb.200110122","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T12:46:23Z","timestamp":1027687583000},"page":"1099-1111","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":1386,"title":["Claudin-based tight junctions are crucial for the mammalian epidermal barrier"],"prefix":"10.1083","volume":"156","author":[{"given":"Mikio","family":"Furuse","sequence":"first","affiliation":[{"name":"1Department of Cell Biology, Kyoto University Faculty of Medicine, Yoshida-Konoe, Sakyo-ku, Kyoto 606-8501, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaki","family":"Hata","sequence":"additional","affiliation":[{"name":"2KAN Research Institute, Inc., Kyoto Research Park, Chudoji, Shimogyo-ku, Kyoto 600-8317, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyoko","family":"Furuse","sequence":"additional","affiliation":[{"name":"2KAN Research Institute, Inc., Kyoto Research Park, Chudoji, Shimogyo-ku, Kyoto 600-8317, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoko","family":"Yoshida","sequence":"additional","affiliation":[{"name":"3Department of Dermatology, Kyoto University Faculty of Medicine, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akinori","family":"Haratake","sequence":"additional","affiliation":[{"name":"4Basic Research Laboratory, Kanebo, Ltd., Kotobuki-cho, Odawara, Kanagawa 250-0002, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshinobu","family":"Sugitani","sequence":"additional","affiliation":[{"name":"5Department of Cell Biology, Japanese Foundation for Cancer Research-Cancer Institute, Kami-Ikebukuro, Toshima-ku, Tokyo 170-8455, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsuo","family":"Noda","sequence":"additional","affiliation":[{"name":"5Department of Cell Biology, Japanese Foundation for Cancer Research-Cancer Institute, Kami-Ikebukuro, Toshima-ku, Tokyo 170-8455, Japan"},{"name":"6Department of Molecular Genetics, Tohoku University School of Medicine, Seryo-cho, Aoba-ku, Sendai 980-8575, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akiharu","family":"Kubo","sequence":"additional","affiliation":[{"name":"1Department of Cell Biology, Kyoto University Faculty of Medicine, Yoshida-Konoe, Sakyo-ku, Kyoto 606-8501, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shoichiro","family":"Tsukita","sequence":"additional","affiliation":[{"name":"1Department of Cell Biology, Kyoto University Faculty of Medicine, Yoshida-Konoe, Sakyo-ku, Kyoto 606-8501, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2002,3,11]]},"reference":[{"key":"2023072213262624400_BIB1","first-page":"G467","volume":"269","year":"1995","journal-title":"Am. J. Physiol."},{"key":"2023072213262624400_BIB2","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1083\/jcb.133.1.43","volume":"133","year":"1996","journal-title":"J. Cell Biol."},{"key":"2023072213262624400_BIB3","doi-asserted-by":"crossref","first-page":"1031","DOI":"10.1083\/jcb.134.4.1031","volume":"134","year":"1996","journal-title":"J. Cell Biol."},{"key":"2023072213262624400_BIB4","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1083\/jcb.138.4.891","volume":"138","year":"1997","journal-title":"J. Cell Biol."},{"key":"2023072213262624400_BIB5","first-page":"156","volume":"162","year":"1987","journal-title":"Anal. Biochem."},{"key":"2023072213262624400_BIB6","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1007\/BF01870332","volume":"39","year":"1978","journal-title":"J. Membr. Biol."},{"key":"2023072213262624400_BIB7","first-page":"10","volume":"20","year":"1977","journal-title":"Physiologist."},{"key":"2023072213262624400_BIB8","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/S0022-2275(20)41520-2","volume":"33","year":"1992","journal-title":"J. Lipid Res."},{"key":"2023072213262624400_BIB9","first-page":"44s","volume":"80","year":"1983","journal-title":"J. Invest. Dermatol."},{"key":"2023072213262624400_BIB10","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1083\/jcb.65.1.180","volume":"65","year":"1975","journal-title":"J. Cell Biol."},{"key":"2023072213262624400_BIB11","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1002\/ar.1091890404","volume":"189","year":"1977","journal-title":"Anat. Rec."},{"key":"2023072213262624400_BIB12","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1083\/jcb.153.2.243","volume":"153","year":"2001","journal-title":"J. Cell Biol."},{"key":"2023072213262624400_BIB13","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1083\/jcb.17.2.375","volume":"17","year":"1963","journal-title":"J. Cell Biol."},{"key":"2023072213262624400_BIB14","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1083\/jcb.26.1.263","volume":"26","year":"1965","journal-title":"J. Cell Biol."},{"key":"2023072213262624400_BIB15","doi-asserted-by":"crossref","first-page":"3443","DOI":"10.1242\/jcs.108.11.3443","volume":"108","year":"1995","journal-title":"J. Cell Sci."},{"key":"2023072213262624400_BIB16","doi-asserted-by":"crossref","first-page":"1777","DOI":"10.1083\/jcb.123.6.1777","volume":"123","year":"1993","journal-title":"J. Cell Biol."},{"key":"2023072213262624400_BIB17","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1242\/jcs.109.2.429","volume":"109","year":"1996","journal-title":"J. Cell Sci."},{"key":"2023072213262624400_BIB18","doi-asserted-by":"crossref","first-page":"1539","DOI":"10.1083\/jcb.141.7.1539","volume":"141","year":"1998","journal-title":"J. Cell Biol."},{"key":"2023072213262624400_BIB19","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1083\/jcb.143.2.391","volume":"143","year":"1998","journal-title":"J. Cell Biol."},{"key":"2023072213262624400_BIB20","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1083\/jcb.147.4.891","volume":"147","year":"1999","journal-title":"J. Cell Biol."},{"key":"2023072213262624400_BIB21","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1083\/jcb.153.2.263","volume":"153","year":"2001","journal-title":"J. Cell Biol."},{"key":"2023072213262624400_BIB22","doi-asserted-by":"crossref","first-page":"1631","DOI":"10.1083\/jcb.123.6.1631","volume":"123","year":"1993","journal-title":"J. Cell Biol."},{"key":"2023072213262624400_BIB23","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1111\/1523-1747.ep12292052","volume":"57","year":"1971","journal-title":"J. Invest. Dermatol."},{"key":"2023072213262624400_BIB24","doi-asserted-by":"crossref","first-page":"6626","DOI":"10.1016\/S0021-9258(18)38163-8","volume":"266","year":"1991","journal-title":"J. Biol. Chem."},{"key":"2023072213262624400_BIB25","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1083\/jcb.121.3.491","volume":"121","year":"1993","journal-title":"J. Cell Biol."},{"key":"2023072213262624400_BIB26","doi-asserted-by":"crossref","first-page":"714","DOI":"10.1111\/1523-1747.ep12470386","volume":"88","year":"1987","journal-title":"J. Invest. Dermatol."},{"key":"2023072213262624400_BIB27","doi-asserted-by":"crossref","first-page":"1044","DOI":"10.1073\/pnas.95.3.1044","volume":"95","year":"1998","journal-title":"Proc. Natl. Acad. Sci. USA."},{"key":"2023072213262624400_BIB28","doi-asserted-by":"crossref","first-page":"2287","DOI":"10.1242\/jcs.109.9.2287","volume":"109","year":"1996","journal-title":"J. Cell Sci."},{"key":"2023072213262624400_BIB29","doi-asserted-by":"crossref","first-page":"862","DOI":"10.1046\/j.1523-1747.1998.00209.x","volume":"110","year":"1998","journal-title":"J. Invest. Dermatol."},{"key":"2023072213262624400_BIB30","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1073\/pnas.96.2.511","volume":"96","year":"1999","journal-title":"Proc. Natl. Acad. Sci. USA."},{"key":"2023072213262624400_BIB31","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1083\/jcb.145.3.579","volume":"145","year":"1999","journal-title":"J. Cell Biol."},{"key":"2023072213262624400_BIB32","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1083\/jcb.147.1.185","volume":"147","year":"1999","journal-title":"J. Cell Biol."},{"key":"2023072213262624400_BIB33"},{"key":"2023072213262624400_BIB34","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1083\/jcb.76.3.705","volume":"76","year":"1978","journal-title":"J. Cell Biol."},{"key":"2023072213262624400_BIB35","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1126\/science.7529942","volume":"267","year":"1995","journal-title":"Science."},{"key":"2023072213262624400_BIB36","first-page":"222","volume":"73","year":"1997","journal-title":"Eur. J. Cell Biol."},{"key":"2023072213262624400_BIB37","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1083\/jcb.141.2.397","volume":"141","year":"1998","journal-title":"J. Cell Biol."},{"key":"2023072213262624400_BIB38","doi-asserted-by":"crossref","first-page":"4131","DOI":"10.1091\/mbc.11.12.4131","volume":"11","year":"2000","journal-title":"Mol. Biol. Cell."},{"key":"2023072213262624400_BIB39","first-page":"L647","volume":"262","year":"1992","journal-title":"Am. J. Physiol."},{"key":"2023072213262624400_BIB40","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1038\/11926","volume":"22","year":"1999","journal-title":"Nat. Genet."},{"key":"2023072213262624400_BIB41","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1126\/science.285.5424.103","volume":"285","year":"1999","journal-title":"Science."},{"key":"2023072213262624400_BIB42","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1083\/jcb.147.1.195","volume":"147","year":"1999","journal-title":"J. Cell Biol."},{"key":"2023072213262624400_BIB43","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/S0022-5320(73)90076-2","volume":"43","year":"1973","journal-title":"J. Ultrastr. Res."},{"key":"2023072213262624400_BIB44","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/S0074-7696(08)60940-7","volume":"39","year":"1974","journal-title":"Int. Rev. Cytol."},{"key":"2023072213262624400_BIB45","doi-asserted-by":"crossref","first-page":"F5","DOI":"10.1083\/jcb.151.2.F5","volume":"151","year":"2000","journal-title":"J. Cell Biol."},{"key":"2023072213262624400_BIB46","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1083\/jcb.103.3.755","volume":"103","year":"1986","journal-title":"J. Cell Biol."},{"key":"2023072213262624400_BIB47","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1016\/S0962-8924(99)01578-0","volume":"9","year":"1999","journal-title":"Trends Cell Biol."},{"key":"2023072213262624400_BIB48","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1083\/jcb.149.1.13","volume":"149","year":"2000","journal-title":"J. Cell Biol."},{"key":"2023072213262624400_BIB49","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1038\/35067088","volume":"2","year":"2001","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"2023072213262624400_BIB50","doi-asserted-by":"crossref","first-page":"1319","DOI":"10.1172\/JCI12464","volume":"107","year":"2001","journal-title":"J. Clin. Invest."},{"key":"2023072213262624400_BIB51","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/S0092-8674(01)00200-8","volume":"104","year":"2001","journal-title":"Cell."},{"key":"2023072213262624400_BIB52","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1083\/jcb.136.2.399","volume":"136","year":"1997","journal-title":"J. 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