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When the tC fusion switch is \u201con,\u201d fusion is much faster. The tC fusion switch can be thrown by a peptide that corresponds to the membrane-proximal half of the cognate v-SNARE, and binds reversibly to the cognate region of the t-SNARE. This structures the coil in the membrane-proximal domain of the t-SNARE and accelerates fusion, implying that the intrinsically unstable coil in that region is a natural impediment to the completion of zippering, and thus, fusion. Proteins that stabilize or destabilize one or the other state of the tC fusion switch would exert fine temporal control over the rate of fusion after SNAREs have already partly zippered up.<\/jats:p>","DOI":"10.1083\/jcb.200112081","type":"journal-article","created":{"date-parts":[[2002,9,3]],"date-time":"2002-09-03T19:13:39Z","timestamp":1031080419000},"page":"929-940","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":180,"title":["Regulation of membrane fusion by the membrane-proximal coil of the t-SNARE during zippering of SNAREpins"],"prefix":"10.1083","volume":"158","author":[{"given":"Thomas J.","family":"Melia","sequence":"first","affiliation":[{"name":"Department of Cellular Biochemistry and Biophysics, Memorial Sloan-Kettering Cancer Center, New York, NY 10021"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Weber","sequence":"additional","affiliation":[{"name":"Department of Cellular Biochemistry and Biophysics, Memorial Sloan-Kettering Cancer Center, New York, NY 10021"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James A.","family":"McNew","sequence":"additional","affiliation":[{"name":"Department of Cellular Biochemistry and Biophysics, Memorial Sloan-Kettering Cancer Center, New York, NY 10021"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lillian E.","family":"Fisher","sequence":"additional","affiliation":[{"name":"Department of Cellular Biochemistry and Biophysics, Memorial Sloan-Kettering Cancer Center, New York, NY 10021"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert J.","family":"Johnston","sequence":"additional","affiliation":[{"name":"Department of Cellular Biochemistry and Biophysics, Memorial Sloan-Kettering Cancer Center, New York, NY 10021"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Parlati","sequence":"additional","affiliation":[{"name":"Department of Cellular Biochemistry and Biophysics, Memorial Sloan-Kettering Cancer Center, New York, NY 10021"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lara K.","family":"Mahal","sequence":"additional","affiliation":[{"name":"Department of Cellular Biochemistry and Biophysics, Memorial Sloan-Kettering Cancer Center, New York, NY 10021"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas H.","family":"So\u0308llner","sequence":"additional","affiliation":[{"name":"Department of Cellular Biochemistry and Biophysics, Memorial Sloan-Kettering Cancer Center, New York, NY 10021"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James E.","family":"Rothman","sequence":"additional","affiliation":[{"name":"Department of Cellular Biochemistry and Biophysics, Memorial Sloan-Kettering Cancer Center, New York, NY 10021"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2002,9,3]]},"reference":[{"key":"2023072213454490700_BIB1","doi-asserted-by":"crossref","first-page":"2520","DOI":"10.1074\/jbc.272.4.2520","volume":"272","year":"1997","journal-title":"J. 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