{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T20:01:28Z","timestamp":1790539288405,"version":"4.1.0"},"reference-count":44,"publisher":"Rockefeller University Press","issue":"2","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2002,10,28]]},"abstract":"<jats:p>The cellular decoding of receptor-induced signaling is based in part on the spatiotemporal activation pattern of PKC isoforms. Because classical and novel PKC isoforms contain diacylglycerol (DAG)-binding C1 domains, they may compete for DAG binding. We reasoned that a Ca2+-induced membrane association of classical PKCs may accelerate the DAG binding and thereby prevent translocation of novel PKCs. Simultaneous imaging of fluorescent PKC fusion proteins revealed that during receptor stimulation, PKC\u03b1 accumulated in the plasma membrane with a diffusion-limited kinetic, whereas translocation of PKC\u03b5 was delayed and attenuated. In BAPTA-loaded cells, however, a selective translocation of PKC\u03b5, but not of coexpressed PKC\u03b1, was evident. A membrane-permeable DAG analogue displayed a higher binding affinity for PKC\u03b5 than for PKC\u03b1. Subsequent photolysis of caged Ca2+ immediately recruited PKC\u03b1 to the membrane, and DAG-bound PKC\u03b5 was displaced. At low expression levels of PKC\u03b5, PKC\u03b1 concentration dependently prevented the PKC\u03b5 translocation with half-maximal effects at equimolar coexpression. Furthermore, translocation of endogenous PKCs in vascular smooth muscle cells corroborated the model that a competition between PKC isoforms for DAG binding occurs at native expression levels. We conclude that Ca2+-controlled competitive DAG binding contributes to the selective recruitment of PKC isoforms after receptor activation.<\/jats:p>","DOI":"10.1083\/jcb.200203048","type":"journal-article","created":{"date-parts":[[2002,10,28]],"date-time":"2002-10-28T20:02:20Z","timestamp":1035835340000},"page":"291-302","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":89,"title":["Ca2+-controlled competitive diacylglycerol binding of protein kinase C isoenzymes in living cells"],"prefix":"10.1083","volume":"159","author":[{"given":"Johannes C.","family":"Lenz","sequence":"first","affiliation":[{"name":"1Institut fu\u0308r Pharmakologie, Freie Universita\u0308t Berlin, 14195 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. 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