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Vacuole fusion is dramatically altered by perturbation of the vacuole-bound actin, either by mutation of the ACT1 gene, addition of specific actin ligands such as latrunculin B or jasplakinolide, antibody to the actin regulatory proteins Las17p (yeast Wiskott-Aldrich syndrome protein) or Arp2\/3, or deletion of actin regulatory genes. On docked vacuoles, actin is enriched at the \u201cvertex ring\u201d membrane microdomain where fusion occurs and is required for the terminal steps leading to membrane fusion. This role for actin may extend to other trafficking systems.<\/jats:p>","DOI":"10.1083\/jcb.200204089","type":"journal-article","created":{"date-parts":[[2002,9,18]],"date-time":"2002-09-18T18:04:15Z","timestamp":1032372255000},"page":"669-679","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":149,"title":["Remodeling of organelle-bound actin is required for yeast vacuole fusion"],"prefix":"10.1083","volume":"158","author":[{"given":"Gary","family":"Eitzen","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naomi","family":"Thorngren","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William","family":"Wickner","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2002,8,12]]},"reference":[{"key":"2023072904455877500_BIB1","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1083\/jcb.200106065","volume":"155","year":"2001","journal-title":"J. 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