{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T23:46:37Z","timestamp":1791416797595,"version":"4.3.3"},"reference-count":40,"publisher":"Rockefeller University Press","issue":"3","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2002,11,11]]},"abstract":"<jats:p>Regulation of membrane-type 1 matrix metalloproteinase (MT1-MMP) by different extracellular matrices (ECMs) on human endothelial cells (ECs) has been investigated. First, MT1-MMP is found at the intercellular contacts of confluent ECs grown on \u03b21 integrin\u2013dependent matrix such as type 1 collagen (COL I), fibronectin (FN), or fibrinogen (FG), but not on gelatin (GEL) or vitronectin (VN). The novel localization of MT1-MMP at cell\u2013cell contacts is assessed by confocal videomicroscopy of MT1-MMP-GFP\u2013transfected ECs. Moreover, MT1-MMP colocalizes with \u03b21 integrins at the intercellular contacts, whereas it is preferentially found with \u03b1v\u03b23 integrin at motility-associated structures on migrating ECs. In addition, clustered integrins recruit MT1-MMP and neutralizing anti-\u03b21 or anti-\u03b1v integrin mAb displace MT1-MMP from its specific sites, pointing to a biochemical association that is finally demonstrated by coimmunoprecipitation assays. On the other hand, COL I, FN, or FG up-regulate cell surface MT1-MMP on confluent ECs by an impairment of its internalization, whereas expression and internalization are not modified on GEL or VN. In addition, MT1-MMP activity is diminished in confluent ECs on COL I, FN, or FG. Finally, MT1-MMP participates and cooperates with \u03b21 and \u03b1v\u03b23 integrins in the migration of ECs on different ECM. These data show a novel mechanism by which ECM regulates MT1-MMP association with \u03b21 or \u03b1v\u03b23 integrins at distinct cellular compartments, thus modulating its internalization, activity, and function on human ECs.<\/jats:p>","DOI":"10.1083\/jcb.200205026","type":"journal-article","created":{"date-parts":[[2002,11,11]],"date-time":"2002-11-11T20:44:40Z","timestamp":1037047480000},"page":"509-521","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":197,"title":["ECM regulates MT1-MMP localization with \u03b21 or \u03b1v\u03b23 integrins at distinct cell compartments modulating its internalization and activity on human endothelial cells"],"prefix":"10.1083","volume":"159","author":[{"given":"Beatriz G.","family":"Ga\u0301lvez","sequence":"first","affiliation":[{"name":"Departamento de Inmunologi\u0301a, Hospital de la Princesa, 28006 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salomo\u0301n","family":"Mati\u0301as-Roma\u0301n","sequence":"additional","affiliation":[{"name":"Departamento de Inmunologi\u0301a, Hospital de la Princesa, 28006 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mari\u0301a","family":"Ya\u0301n\u0303ez-Mo\u0301","sequence":"additional","affiliation":[{"name":"Departamento de Inmunologi\u0301a, Hospital de la Princesa, 28006 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco","family":"Sa\u0301nchez-Madrid","sequence":"additional","affiliation":[{"name":"Departamento de Inmunologi\u0301a, Hospital de la Princesa, 28006 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alicia G.","family":"Arroyo","sequence":"additional","affiliation":[{"name":"Departamento de Inmunologi\u0301a, Hospital de la Princesa, 28006 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2002,11,11]]},"reference":[{"key":"2023072213502502900_BIB1","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1083\/jcb.117.3.659","volume":"117","year":"1992","journal-title":"J. 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